STUDIES ON EXPRESSION OF INSULIN LIKE GROWTH FACTOR BINDING PROTEIN 3 (IGFBP 3) GENE IN CULTURED AND IN VIVO DEVELOPED PREANTRAL FOLLICLES IN SHEEP
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Date
2010-10
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Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P
Abstract
ABSTRACT:
Expression of Growth Differentiation Factor 9 (GDF 9) and Insulin like Growth Factor Binding Protein 3 (IGFBP 3) genes was studied in oocytes and granulosa cells isolated from various in vivo and in vitro follicular development stages in sheep. In the present study, the expression of both the genes was highest in the primordial follicles. The expression of GDF 9 in in vivo and in vitro oocytes decreased as the development progressed except in large antral follicles (OA2). Among the granulosa cells, the expression of GDF 9 in in vivo stages decreased continuously. In the in vitro granulosa cell stages, the expression of GDF 9 increased except PFs’ cultured for 6 days (G6). The expression of IGFBP 3 in in vivo and in vitro oocytes increased as the development progressed except in antral follicles (OA1). Among the granulosa cells, the expression of IGFBP 3 in in vivo and in vitro stages increased as the development progressed except PFs’ cultured for 6 days (G6). When the oocytes from large antral follicles (OA2) and 6 day cultured PFs’ (O6) were matured in vitro for 24h, the expression of both the genes decreased. When the granulosa cells from large antral follicles (GA2) and 6 day cultured PFs’ (G6) were matured in vitro for 24h, the expression of both the genes increased. The expression of both the genes increased significantly in the oocytes and granulosa cells from in vitro cultured PFs’ than their corresponding in vivo stages