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2010 - 20113
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End date: 2011 × Start date: 2010 × Thesis Type: M.V.Sc ×
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    ThesisItemOpen Access
    EPIDEMIOLOGICAL STUDIES ON PESTE DES PETITS RUMINANTS IN CERTAIN PARTS OF ANDHRA PRADESH
    (Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2011) SARITHA, G; SHOBHAMANI, B (Major); SREEDEVI, B; SREENIVASULU, D
    Abstract: Peste des petits ruminants (PPR) is an acute highly contagious and rapidly spreading transboundary viral disease of sheep and goats. The disease is associated with high morbidity and mortality in susceptible animals. It causes severe losses to small ruminant production. Hence a modest attempt has been made to study PPR in Andhra Pradesh with respect to epidemiological status, sero-prevalence, antigen detection and evaluation of efficacy of PPR live attenuated vaccine. The sero-prevalence of PPR was studied by screening 803 sera samples from sheep (398) and goats (405) belonging to 5 different districts of Andhra Pradesh during the period from December 2010 to April 2011. The overall sero-prevalence of PPR in sheep and goat of the present study was 67.87 per cent. Among the districts sero-prevalence ranged from 49.25 to 89.07 per cent. Species wise sero-prevalence of PPR in sheep and goats was 65.32 and 70.37 per cent, respectively. The sero-prevalence was higher (80.65%) in 13-45 months old followed by 4-12 months (67.83%) old sheep and goat. Out of 195 clinical samples (nasal swabs and tissues) tested, the PPR antigen was detected in 66 samples with an overall prevalence of 33.84 per cent. The antigen prevalence (tissues and swabs) was relatively higher (34.52%) in goats than in sheep (33.33%). In the present study 47 flocks (94%) were found positive for PPR antibodies out of 50 flocks (sheep and goats) screened, whereas both the antigen and antibody were detected in 29 flocks (69.04%) out of 42 flocks tested. However in 3 flocks neither antigen nor antibody was detected. Reference vaccine virus as well as clinical samples, including nasal swab and liver tissue showed amplification of approximately 351 bp which is specific to PPRV. However no amplification was observed in the nasal swab collected from apparently healthy sheep. Antibody response to PPR live-attenuated vaccine was studied at Livestock Research station, Palamaner. Results showed that mean PI values in all age groups (G-I, G-II and G-III) gradually increased until 90 days (maximum period of observation) of post immunization, indicating initiation of antibody response to vaccine in field condition. Further studies are required to determine the duration of immunity of PPR live attenuated vaccine.
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    ThesisItemOpen Access
    IMPACT STUDIES ON THE ESTABLISHMENT OF BULK MILK COOLING UNITS IN SELECTED DISTRICTS OF ANDHRA PRADESH
    (Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2010-12) POORNACHANDRA RAO, K.N; SURESH, J (Major); SARJAN RAO, K; VEERABRAHMAIAH, K
    ABSTRACT : The study had a focus on the impacts of establishment of bulk milk cooling units both on quality of milk as well as socio-economic impact on the milk producer community in Chittoor and Prakasam districts of Andhra Pradesh. The research was conducted by adopting ex-post-facto design. Of the 56 units functioning in the Chittoor district, 9 units were randomly selected while only BMC functioning in Prakasam district was selected purposively. The list of milk producers supplying milk to the BMC unions was prepared and a uniform sample of 15 farmers comprising 10 female respondents and 5 male respondents were randomly selected. The ultimate sample for the study stood at 10 BMC’s and 150 respondents. The required information was collected by personal interview by the researcher with the respondents through a structured schedule. The data were subjected to statistical analysis employing means, standard deviation, percentages, Chi-2 test of significance, multiple regression analysis and correlation coefficients. To study the effectiveness of bulk milk coolers quantitative methods were employed to measure the standard plate count (SPC) and coliform count (CC) before and after cooling the milk in bulk milk coolers along with fat and SNF percentages. The study regarding the effectiveness of bulk milk coolers on the quality of milk revealed that there was substantial reduction in SPC and coliform count after cooling. The percentage reduction of SPC and CC was approximately 36% and 43%, respectively. However the quality of milk by grade remained fair as per BIS even after cooling with BMC units. There was a significant difference (P<0.01) with regard to SPC and CC before and after subjecting the milk through BMC’s both in terms of maximum and minimum counts. The annual milk collection through BMC units ranged from a minimum of 5,21,000 litres to a maximum of 16,91,000 litres. The mean quantity of milk collected through all BMC units was 10,35,000 litres. The BMC’s have approximately took away 17% of share of milk procurement from different channels like head loads, private dairies etc. This indirectly will strengthen the fact that quality milk of low bacterial load to the extent of 17% is now being supplied to the consumers compared to the earlier situation. X2 analysis indicated that the relation between milk yield with some of the socio-economic parameters like education, size of the farm, total household income and quality of concentrate feed offered to the animal were found to be significant (P<0.05). The multiple regression analysis carried out to study the influence of selected independent variables and dairy income due to establishment of BMC units revealed that in respect of pooled sample, the most powerful factors to influence the dairy income were caste, hand holdings, size of the farm, education level, household income and dairy farming experience. The coefficient of multiple determination was 0.67 duly indicating that 67% of the variation in dairy income was explained by the independent variables included in the function. The correlation coefficients between dairy income due to establishment of BMC units and selected socio-economic parameters pertaining to pooled sample revealed that size of the dairy herd had highest association with dairy income, very closely followed by farming experience and land holding. Household income and level of education also exhibited positive correlations with dairy income. The impact of BMC units as perceived by the respondents revealed that tie-up for bank loans has been an easy affair and this item obtained first rank. There has been a substantial increase in dairy income brought in due to BMC’s and it was offered second rank. Another important impact was fairer payment for the milk supplied which was ranked three. Spoilage of milk was averted to a great extent (rank IV). BMC’s enabled the farmers to demand for higher milk price which was ranked fifth. Increase in milk quality was yet another benefit of BMC. Other interesting impacts include society development, increased milk production improvement in health of the animals, increase in milk production, increase in herd size and improved extension contacts. Some important constraints faced by the beneficiaries were brought to the lime light and few of them required to be addressed immediately by the authorities. Irregular power supply which may pose a threat of spoilage of milk was the prime concern. Setting up of a generator as a back-up facility was the suggestion. Under-utilization of coolers to the fullest capacity of either 3,000 or 5,000 litres especially during lean months resulted in increased overhead charges. It was suggested that more number of milk producers be inducted into the BMC union and others are encouraged to divert their milk supply from head loads and private dairies to BMC’s. Further, the farmers are expecting more training programmes on production technologies, better extension services, organization of animal health camps and loan melas etc. to help them to produce more milk per unit animal.
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    ThesisItemOpen Access
    HISTOPATHOLOGICAL, IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES ON ENDOMETRIAL BIOPSIES IN INFERTILE BUFFALOES
    (Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P, 2010-01) SAMATHA, V; SRILATHA, Ch (Major); ANJANEYULU, Y; SURESH KUMAR, R.V
    ABSTRACT : Buffaloes play an important role in dairy developmental programmes of our country. In spite of tremendous advances in veterinary medicine, during the past 50 years, the infertility in bovines remained as almost important economic factor. Poor reproductive efficiency is one of the major problems faced by buffalo breeders. Among various pathological conditions of female reproductive tract, inflammation and unfavourable uterine environment is the most important etiological factor for infertility in bovines. Endometrial biopsy examination is the most reliable diagnostic tool for veterinarians to identify the nature of infertility and so aid in reproductive herd health programme. It can be used to identify histopathological changes of endometrium and thus serve as a basis for prognostic evaluation of infertile animals. Diagnosis of sub clinical cases is possible only by histopathological examination of uterine biopsy samples. The isolation of microorganisms along with histopathological studies of endometrial biopsy permits more accurate assessment of reproductive prognosis and rational treatment of reproductive breeding of animals. For the present study, one hundred and ten endometrial biopsies samples were collected from infertile buffaloes after thorough rectal examination and in absence of palpable uterine abnormalities. Biopsies were collected aseptically by Albuchins uterine biopsy catheter after induction of epidural anaesthesia under sterile conditions. Uterine aspirate was collected aseptically and carefully by sterile uterine catheter for cytological examination. Histopathological, cytological, bacteriological, immunohistochemical and electron microscopic studies were carried out on uterine biopsies. Histopathologically, the lesions observed were acute, subacute, chronic, chronic suppurative and chronic catarrhal changes in 14.54%, 34.54%, 46.37%, 1.82% and 2.73% biopsies respectively. Acute endometritis cases revealed severe congestion of endometrial blood vessels, stromal edema, degenerative changes in luminal epithelium, focal areas of denudation of epithelial lining and infiltration of polymorphonuclear cells and few lymphocytes. Endometrial cytological smears in acute endometritis cases revealed more number of neutrophils. Moderate infiltration in sub epithelial zone of stratum compactum, moderate periglandular fibrosis, hypertrophy of blood vessels, cystic dilation of endometrial glands in addition to stromal edema and glandular edema were noticed in sub acute endometritis. Lymphocytes and polymorphonuclear cells were observed in addition to epithelial cells in endometrial cytology of sub acute endometritis cases. Diffuse infiltration of lymphocytes, plasma cells and macrophages in stratum compactum and stratum spongiosum, thick fibro cellular endometrial stroma and gland site masses with severe periglandular fibrosis were observed in chronic endometritis cases. Severe proliferation of fibroblasts, glandular sclerosis and hyalinization of blood vessels were also noticed in chronic endometritis. Few chronic endometritis cases revealed cystic dilatation of endometrial glands and glandular hyperplasia in other non affected glands. Endometrial cytology of chronic endometritis cases revealed lymphocytes, few plasma cells in addition to epithelial cells and mucin strands. Chronic suppurative endometritis cases revealed periglandular fibrosis and infiltration of more polymorphonuclear cells in stratum compactum and stratum spongiosum. More number of degenerating polymorphonuclear cells in addition to colonies of cocco-bacilli bacteria were observed in endometrial cytological smears of chronic suppurative endometritis. Chronic catarrhal endometritis cases revealed catarrhal changes in luminal epithelium and thick connective tissue stroma in endometrium. Bacteriological examination of endometrial biopsy samples yielded majorily Salmonella (32.5%) followed by E.coli (23.75%), Staphylococcus (28.75%), Pseudomonas (8.75%), Klebsiella (2.5%), Pasteurella (2.5%) and Streptococci (1.25%). Immunohistochemical studies of chronic endometritis biopsies revealed more number of CD3 positive cells (pan T lymphocytes) in stratum compactum. Six chronic endometritis biopsies revealed CD138 positive cells (plasma cells) in endometrial stroma. Scanning electron microscopic examination of uterine biopsies collected from normal endometrium revealed surface epithelial cells with few ciliated and non ciliated cells. Loss of cilia and microvilli of surface epithelium was observed in acute endometritis cases .Sub acute endometritis cases revealed rod shaped bacteria adhering to surface epithelial cells and damaged epithelial cells with loss of microvilli. Destruction of surface epithelium and glandular structure leaving hole like spaces and fibrosis with thin long reticulin fibers were noticed in chronic endometritis cases.