Loading...
Thesis
Browse
38 results
Search Results
ThesisItem Open Access NUTRITIONAL EVALUATION OF APBN1 GRASS AND EFFECT OF PROTEIN SUPPLEMENTATION ON THE PERFORMANCE OF DECCANI SHEEP(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2005-06) JAGADISH, Ch; RAMANA REDDY, Y(MAJOR); NAGALAKSHMI, D; MAHENDER, MABSTRACT : Evaluation of the potential feeding value of APBN1, an improved variety of hybrid napier as the sole feed and in supplemented rations was carried out to develop a forage based feeding system for intensive rearing of sheep. APBN1 forage samples harvested at 30, 40, 45, 50 and 60 days of growth were subjected to chemical, in vitro and in sacco evaluation to determine the optimum stage of harvesting for maximization of forage utilization in terms of fodder yield and nutritional quality. The yield of green fodder, dry matter and crude protein (MT/ha/yr) of APBN1 forage harvested at 30, 40, 45, 50 and 60 days of growth was found to be 274.68, 47.35, 7.44; 291.34, 61.09, 9.08; 344.74, 76.39, 11.45; 368.45, 85.26, 11.36 and 387.95, 98.81 and 11.21, respectively. The leaf to stem ratio of APBN1 forage at 30, 40, 45, 50 and 60 days of growth was found to be 0.36, 0.46, 0.65, 0.95 and 1.36, respectively. The dry matter content of forage increased from 17.24 per cent at 30 days to 25.47 per cent at 60 days of growth. The CP and CF values were 15.72, 23.81; 14.86, 24.67; 14.35, 25.32; 13.68, 26.64 and 11.34 and 29.88 per cent, respectively on DM basis for the forage samples harvested at 30, 40, 45, 50 and 60 days of growth. Progressive increase in crude fibre level and gradual decline in crude protein content was observed with the advancement in maturity of forage. The NFE content (46.12 %) was highest at 45 days of growth. The IVDMD value was highest (57.87 %) at 30 days and lowest (51.40 %) at 60 days of growth, when the forage samples were incubated with sheep rumen liquor. The effective degradable dry matter (EDDM) as determined by nylon bag technique in fistulated rams was 55.80, 54.70, 53.50, 52.40 and 43.78 per cent at 30, 40, 45, 50 and 60 days of growth, respectively. The effective protein degradability (EPD) decreased from 38.30 per cent at 30 days to 33.17 per cent at 60 days of growth. The insoluble but degradable protein fraction „b‟ was highest (77.07 %) at 45 days of growth. Based on the biomass yield, leaf to stem ratio, chemical, in vitro and in sacco evaluation, the optimum stage of harvesting for APBN1 forage was found to be at 45 days of growth. When the forage harvested at 45 days alone was fed ad libitum in chopped form, the rams on an average consumed 50.74, 4.40 and 28.34 g/kg W0.75 of DM, DCP and TDN respectively. The average digestibility coefficients of 66.63, 68.52, 69.10, 58.82, 56.34 and 62.21 per cent were observed for DM, OM, CP, EE, CF and NFE, respectively and the DCP and TDN values as 8.68 and 56.38 per cent were observed for APBN1 forage in Deccani rams. The Deccani rams on an average retained 5.28 g nitrogen per day. Effect of supplementation through protein and energy sources to the APBN1 forage was studied in adult Deccani rams. There was a significant increase in DM intake (g/kg W0.75) of rams supplemented with concentrate premix containing GNC + maize (71.10), SFC + maize (69.82) and CSC + maize (66.71) in comparison to that of the rams fed APBN1 forage (51.62) as the sole feed. Irrespective of the protein source, supplementation has enhanced the nutrient digestibility of rations in rams with the resultant improvement in DCP content of the ration by 72.78% (GNC), 35.64% (CSC) and 34.95% (SFC) and TDN value of ration by 26.80% (GNC), 21.59% (CSC) and 13.66% (SFC) in comparison to DCP (8.68%) and TDN (56.38%) of the ration containing APBN1 forage alone. There was a marked improvement (P<0.05) in nitrogen utilization with the net nitrogen retention (g/d) in rams supplemented with GNC + maize (15.13), SFC + maize (12.16) and CSC + maize (10.71) when compared to that of rams fed APBN1 forage (5.28) as the sole feed. It is concluded that APBN1 forage at 45 days of growth with DCP (8.68%) and TDN (56.38%) can be utilized as a potential maintenance type (perennial) green fodder for feeding adult sheep. Supplementation, irrespective of protein source, improved the nutrient intake, nutrient digestibility and nitrogen utilization of the rations containing APBN1 forage as the basal roughage and fulfilled the maintenance as well as growth requirements (70 g/d) of sheep. Supplementation through concentrate premix containing 125 g each of maize and GNC was found superior (P<0.05) in enhancing nutrient digestibility and nitrogen utilization of rams in comparison to other sources. Supplementation of critical nutrients to the basal roughage is a viable means of sustaining the optimum animal performance.ThesisItem Open Access HYDROGENATED VEGETABLE OIL : WHETHER BENEFICIAL OR HARMFUL – AN INSIGHT(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-07) ALPHA RAJ, M; GOPALA REDDY, A(MAJOR); ADILAXMAMMA, K; RAJASEKHAR REDDY, A; ANJANEYULU, YABSTRACT : An experimental study was conducted to evaluate the effects of supplementation of high energy high hydrogenated vegetable oil diet in various stressor models. A total of 160 White Leg Horn layers of 18 weeks age were randomly divided into eight groups of 20 birds each. Groups 1, 3, 4 and 5 were maintained on basal diet and groups 2, 6, 7 and 8 on 5% vanaspati supplemented basal diet until the age of 42 weeks. At 42 weeks of age, groups 3 and 6 were treated with 1% ferrous sulphate in the diet, groups 4 and 7 were treated with chlorpyrifos @ 100 ppm and groups 5 and 8 were treated with cadmium @ 100 ppm in the basal diet for a period of 12 weeks. The performance parameters like body weights, weight gains, feed consumption, feed conversion ratio, egg production and egg weight were recorded at monthly intervals. The sero-biochemical analysis of cholesterol, HDL cholesterol, triglycerides, total protein, albumin, globulin, A/G ration, alkaline phosphatase, alanine transaminase and creatinine was carried out at monthly interval. Oxidant and antioxidant status was determined by estimation of TBARS and GSH in serum, liver and kidney, protein carbonyls in serum and vitamin C levels in liver and kidney. The immunological status of the birds was evaluated by HI titre against RD virus in serum and phytohaemagglutinin (PHA) assay at the end of the experimental period. Histopathological studies were conducted on liver, kidney and brain at the end of the experimental period. The ferrous sulphate toxic control revealed significantly (p<0.05) higher FCR, serum creatinine, liver TBARS and phospholipids, and significantly lower egg production compared to normal group. Histopathology revealed congestion and bile duct hyperplasia in liver, intertubular haemorrhage and focal lymphoid aggregates in kidney and no detectable abnormalities in brain. The supplementation of vanaspati along with ferrous sulphate significantly (p<0.05) increased the egg production and serum ALP activity but decreased egg weight, FCR and HI titre compared to toxic control. The histopathology revealed similar changes of more severity besides tubular rearrangement of hepatic cells and degenerative changes in kidney. The CPS toxic control showed significantly (p<0.05) higher egg production, higher serum ALP activities and a non-significantly higher total lipids of liver and lower serum HDL compared to normal group. The histopathology revealed mild fatty changes, congestion and bile duct hyperplasia in liver, degenerative changes in kidney and congestion in brain. Supplementation of vanaspati along with CPS resulted in decreased egg production, decreased egg weight, HI titre but increased PHA index compared to toxic control. The histopathology showed marked fatty changes in liver, haemorrhages in kidney and vacuolation in brain. The cadmium toxic control revealed a significantly (p<0.05) lower body weight, weight gain, egg production and egg weights, but significantly increased the FCR, serum ALP and ALT acitivities, creatinine and TBARS of liver and kidney. The GSH and vitamin C levels of kidney were reduced. Paradoxically, the HDL and HI titre were significantly (p<0.05) increased compared to normal group. The histopathology revealed congestion, lymphoid aggregates and degenerative changes in liver, haemorrhages and congestion in kidney and brain. The supplementation of vanaspati along with cadmium resulted in a significant decrease of liver GSH and decrease of HI titre compared to toxic control. The histopathology revealed similar pathological changes but of more severity along with fibrosis in liver, kidney and brain. The overall effect of vanaspati supplementation revealed significantly (p<0.05) increased egg production and PHA index, and decreased egg weights, FCR, serum cholesterol, albumin, TBARS of liver and kidney, GSH of liver and HI titre compared to basal diet fed group. Thus, it is concluded that ferrous sulphate, chlorpyrifos and cadmium induce biological damage by means of oxidative stress and organ damage. However, cadmium was more potent followed by ferrous sulpahte and chlorpyrifos. After accounting for increased calorie intake, the supplementation of vanaspati was not effective in reducing the toxicity of different stressors and accentuated the toxicity in certain conditions. Further, vanaspati supplementation resulted in abnormal immunological response. In view of the above findings, it is concluded that hydrogenated vegetable oil is harmful and regular use will lead to adverse consequences.ThesisItem Open Access CLINICAL STUDIES ON POSTPARTURIENT INDIGESTION WITH PARTICULAR REFERENCE TO HEPATIC DISORDERS AND PRODUCTION DISEASES IN BUFFALOES(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-06) PADMAJA, K; TIRUMALA RAO, D.S(MAJOR); AMEER HAMZA, P; SADASIVA RAO, K; GOPALA REDDY, AABSTRACT: The present investigation on Postparturient Indigestion (PPI) was undertaken to study the incidence, clinical and biochemical findings, therapeutic efficacy of certain drugs and to evolve a suitable comprehensive therapeutic and prophylactic measures for prevention of PPI in buffaloes associated with hepatic disorders and production diseases. Out of 320 recently calved (zero to two months after calving) buffaloes with the history of reduced feed intake and decreased milk yield presented at Ambulatory Clinic, Mylardevpally, Campus Veterinary Hospital, College of Veterinary Science, Rajendranagar and a few peri urban dairy farms located in and around Rajendranagar, Hyderabad, 90 (28.13%) buffaloes were found suffering with PPI. On detailed clinical examination and urine analysis of cases of PPI, it was observed that 43 (47.78%) buffaloes had hepatic insufficiency, 27 (30 %) had production diseases and 20 (22.22 %) had PPI alone. Out of 43 buffaloes of hepatic insufficiency, 23 (25.56 %) had hepatic insufficiency alone, 20 (22.22 %) had hepatic insufficiency and production diseases, 10 (11.11 %) had hepatic insufficiency with sub clinical hypocalcaemia and 10 (11.11 %) had hepatic disorders with sub clinical ketosis. Out of 27 buffaloes with incidence of PPI in relation to production diseases, 14 (15.56 %) had sub clinical hypocalcaemia and 13 (14.44 %) had sub clinical ketosis. Based on the urine analysis, 90 PPI animals were divided into five groups IIa, IIb, III, IV and V. These five groups were subdivided into two each viz., IIa1, IIa2, IIb1, IIb2, IIIa, IIIb, IVa, IVb, Va and Vb for conducting therapeutic trials. Ten apparently healthy postparturient buffaloes (Group I) were subjected to clinical observations and biochemical investigations, and they served as healthy control group. The most common and consistent symptom observed in buffaloes suffering with PPI was reduction in feed intake and decreased milk yield. Scanty dung was observed in few cases. Milk samples of all the 90 cases of PPI were found negative for ketones before and after treatment. There was no significant difference in the values of temperature, pulse and respiratory rate of PPI animals before and after treatment, which indicated that PPI had no significant effect on the above parameters. All the animals during the treatment regained the appetite and milk yield was increased. Ruminal motility was increased significantly (P<0.01). Urine analysis was normal in all the groups after the treatment. The buffaloes with PPI were administered different therapeutic regimens. Animals belonging to group IIa were low in urinary calcium and hence, 450 ml of Calboral i/v and 300 g of Calup gel P.O was administered for two days in groups IIa1 and IIa2, respectively. Significant improvement was observed in both the groups. Based on the ANOVA results and comparative means, the treatment of group IIa2 was more effective. The group IIb animals were found positive for urinary ketones hence, Rintose 0.50 g per kg b wt i/v was administered for two days. In addition, Laurabolin 100 mg i/m single dose and Ketonex boli 4 per day P.O for three days was given to groups IIb1 and IIb2, respectively. The mean serum glucose and calcium levels increased significantly at 1% (P<0.01) and 5 % (P<0.05), in groups IIb1 and IIb2, respectively. However, the mean serum GGT and total bilirubin levels decreased significantly (P<0.01) in group IIb2. Though there was an improvement in both the groups, the comparative means of serum glucose of healthy, group IIb1 and IIb2 revealed that the treatment given to group IIb2 was more effective. Group III animals were positive for urinary bile pigments. Hence, Rintose 0.50 g per kg b wt i/v was administered for one day. In addition, Livadex forte 10 ml i/m and Toxol 10 ml i/m for three days was given in groups IIIa and IIIb, respectively. The mean serum glucose and total protein levels were increased significantly (P<0.01) in group IIIa animals. There was a significant (P<0.01) decrease in serum AST values, in groups IIIa and IIIb, whereas the mean GGT values decreased significantly (P<0.01) in group IIIb animals. The comparative means of serum AST and GGT of healthy, group IIIa and IIIb revealed that the efficacy of both the treatments was same. Animals belonging to group IV were positive for urinary bile pigments. In addition, IVa was low in calcium, IVb was positive for ketone bodies in urine. The animals of IVa were administered Rintose 0.50 g per kg b wt i/v, Tribivet 10 ml i/m for three days and 300 g of Calup gel P.O for two days. Group IVb animals were administered Rintose 0.50 g per kg b wt i/v for three days, Laurabolin 100 mg i/m single dose and 2 boli of Zigbo P.O. for five days. The mean serum glucose, total protein, albumin and calcium increased significantly (P<0.01) in group IVa animals, whereas the mean serum AST, GGT and total bilirubin decreased significantly (P<0.01). In group IVb animals, the mean total protein, albumin, calcium and phosphorous levels increased significantly (P<0.01), whereas the mean serum AST, GGT and cholesterol levels decreased significantly (P<0.01). Both the treatments were equally effective since the levels of serum AST and GGT were significantly (P<0.01) decreased after therapy. 73 Group V animals were normal in terms of urine analysis. However, rumen liquor analysis revealed alkaline indigestion with mean pH of 8.00±0.00. They were administered Rintose 0.50 g per kg b wt i/v and Anistamin 10 ml i/m for one day. Besides, bolus Ecotas 2 per day P.O. for four days and bolus Rumentas 4 per day (2 boli BID) P.O. for three days were given in groups Va and Vb, respectively. The mean values of serum calcium and albumin increased significantly (P<0.01) in group Va animals. The comparative means of serum glucose and calcium of healthy, group Va and Vb revealed that the efficacy of treatment provided to group Va was more effective. Therefore, it can be concluded that in the treatment of PPI in buffaloes, hepatoprotective and liver stimulant drugs along with i/v administration of glucose, oral calcium preparation and a combination of probiotics and prebiotics are recommended. Urine analysis can be considered as a diagnostic tool for early detection of PPI in buffaloesThesisItem Open Access GENETIC STUDIES ON GROWTH, PRODUCTION AND IMMUNOCOMPETENCE OF JAPANESE QUAILS (Coturnix coturnix japonica) UNDER SELECTION(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009) SAKUNTHALA DEVI, K; RAMESH GUPTA, B(MAJOR)ABSTRACT : An investigation was made on the Black (3,438) and Brown (3,710) Japanese quails (Coturnix coturnix japonica) under selection in generations 8, 9 and 10 for high 4-week body weight, maintained at the Poultry Experimental Station (PES), Rajendranagar, Hyderabad, to estimate the genetic and phenotypic variation in the growth, production and reproduction performance and to estimate the genetic progress in various economic traits. The immunocompetence levels, genetic parameters and genetic progress in various traits were estimated. Selection indices based on body weights, Age at first egg, Egg production, Egg weight and Egg mass were developed. The overall least squares means for body weights at day-old to 4 weeks of age were 9.41+0.01, 33.23+0.08, 71.61+0.16, 121.96+0.24 and 173.66+0.07 g respectively. The strains had significant influence on body weights from day-old to 4 and 8 to 20 weeks of age, whereas the influence of generations was significant from day-old to 16 weeks of age. Sex effect was significant on 2, 3 and 4 weeks body weights, while hatch had significant influence from day-old to 20 weeks of age. The body weights of Black strain were superior to Brown from 1 to 20 weeks of age. The overall least squares mean Age at first egg was 56.26+0.51 days, which was significantly influenced by generation and hatch. Strain and generations influenced the egg production upto 16 and 30 weeks, whereas hatch effect was significant on egg production upto 30 weeks only. The overall least squares means for egg production was 30.85 and 73.02 upto16 and 30 weeks of age, respectively. The Black strain matured earlier and produced more number of eggs than Brown strain. The generation and hatch had significant influence on egg weight. The overall least squares mean egg weight was 14.22 g. The overall least squares means for Average daily gain during 1, 2, 3 and 4 weeks of age and cumulative growth rate from 1 to 4 weeks age were 3.10, 5.47, 7.16, 7.35 and 23.46 g respectively. The Average daily gain of Black Japanese quails was significantly higher than those of Browns at all the ages. The females had significantly higher average daily gains than males. The overall least squares means for feed conversion ratio upto 6 weeks age, feed intake per dozen eggs and kg egg mass upto 7-30 weeks age were 1.70, 2.49, 2.95, 3.52, 4.67, 6.30, 750.93 and 4,070.05 g respectively. The influence of sex was significant on Feed conversion ratio. The overall least squares means for HA titers at 5th and 10th day of post inoculation and Foot pad index were 4.48, 2.00 and 0.16 in Black and 5.44, 2.14 and 0.26 mm in Brown strain respectively. The heritability estimates of body weights varied from low to high, while they were low for age at first egg in Black (0.04 to 0.13) and Brown (0.07 to 0.15). The estimates for egg production upto 30 weeks of age varied 0.02 to 0.08 in Black and from 0.02 to 0.07 in Browns. The heritability of egg weight was found to be low. The heritability estimates for feed conversion ratio were low to medium in Black (0.03 to 0.32) and low to high (0.02 to 0.59) in Browns. The Haemagglutination titer has low to medium (0.18 to 0.35) heritabilities in both the strains, whereas Foot pad index was low heritable (0.02 to 0.04). Genetic, phenotypic and environmental correlations for body weight at various ages varied from low to high in magnitude. Age at first egg was correlated negatively (-0.11 to -0.16) with egg production and positively with egg weight at genetic level, while the estimates among different ages of egg production were high. The association between egg production and egg weight was negative at genetic and phenotypic level. The genetic correlations among average daily gains were in general positive at all ages. The genetic correlation between body weights and Feed conversion ratio were positive, while the weight gain was phenotypically negatively correlated with feed conversion ratio. The genetic correlations of body weights at early age with Haemagglutination titers and FPI were positive but low. The Foot pad index had positive genetic correlation with Haemagglutination titers in two strains. The genetic progress in body weight at 4-weeks age varied from 6.25 to 6.74 g in Black and from 7.24 to 8.46 g in Brown strains. The linear regression equations were fitted for predicting the Haemagglutination titers and Foot pad index based on the body weights which had lower R2 - values. A total of 112 selection indices were computed based on the body weights at 1, 2, 3 and 4 weeks of age and dams AFE, dams EP16, dams EW16 and dams EM16. At 4-weeks of age of selection the selection indices consisting of BW4, dams EP and dams EM (I52) and BW4, dams AFE and dams EM (I49) in Black strain and those with BW4, dams EP16 and dams EM16 (I52) and BW4, dams AFE, dams EP16 and dams EM16 in Brown strain were found to be ideal ones. These indices are expected to reduce the AFE, increase the EM (which takes care of EP and EW) and keep the body weight either constant, reduce marginally or increaseThesisItem Open Access GROSS, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE MALE REPRODUCTIVE SYSTEM OF THE EMU (Dromaius novaehollandiae)(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-05) RAJENDRANATH, N; CHANDRASEKHARA RAO, T.S(MAJOR); PRAMOD KUMAR, D; RAGHAVENDAR, K.B.P; GIRISH KUMAR, VABSTRACT: The present work was undertaken on six breeding season and twelve non breeding season male emu birds to study, Gross, histology and histochemistry of reproductive system. The male genital system of the emu consisted paired testes, epididymal region, ductus deferens and a copulatory apparatus. The testes were black in colour. The left testis was bean shaped and larger while right testis was round and smaller. The epididymal region was located on the dorsomedial aspect of the testis. The ductus deferens showed straight tube, receptacle, ejaculatory duct and a papilla in a sequence from epididymal region to urodeum. The copulatory apparatus of emu consisted of an intromittent phallus and its associated structures ejaculatory groove region, paired retractor penis cranialis and caudalis muscles and paired paracloacal vascular bodies. The phallus consisted of a base and a free part with a blind tubular cavity inside. The base was conical shaped and formed by smaller right and larger left basal fibrolymphatic bodies. The free part of phallus consisted of an evertable pars cavernosa and non evertable pars glandularis. The ejaculatory groove region was a triangular area supported by a fibrocartilage. It was continued as spiral ejaculatory groove between two basal fibrolymphatic bodies. The retractor penis cranialis muscle was larger while the retractor penis cuadalis was smaller in size. The paracloacal vascular bodies were ellipsoid lymphatic structures located below the base of the phallus. There was a significant increase in size and length of the different structures of male reproductive system of emu during breeding season (October – February) compared to Non-breeding season (March – September). Despite the presence of small amount of septa there was no distinct lobulation of the testis melanocytes scattered in the capsule and interstitial tissue were responsible for the dark coloration of the testes. The rete testis was composed of intratesticular, intracapsular and extra testicular portion. The intratesticular rete was lined by a single layer of cuboidal or columnar cells. The intracapsular and extra testicular rete lined by simple cuboidal epithelium. The ductules of the epididymal region were lined by pseudostratified columnar epithelium with ciliated and non ciliated cells. The ductus deferens was lined by pseudostratified nonciliated columnar epithelium. The surfaces of basal fibrolymphatic bodies were lined by stratified squamous cornified epithelium which was changed to pseudostratified columnar type in deeper parts of ejaculatory groove.ThesisItem Open Access STUDIES ON THE INFLUENCE OF UDDER MORPHOLOGY, METHOD OF MILKING AND HOUSING SYSTEMS ON MILKING CHARACTERISTICS, UDDER HEALTH AND BEHAVIOUR OF MURRAH BUFFALOES(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-05) Maruthivara Prasad, R; Sudhakar, K(MAJOR); Raghava Rao, E; Ramesh Gupta, B; Mahendar, MABSTRACT: An investigation was undertaken to study the influence of udder morphology, method of milking and housing systems on the milk yield, udder health and behaviour in Murrah buffaloes. In this study Murrah buffaloes maintained at the Buffalo Research Station, Venkataramannagudem, Sri Venkateswara Veterinary University and in the field were utilized. A total of 200 buffaloes were considered to study the udder and teat morphology with 100 buffaloes each from research station and field. The individual behavioural responses of Murrah buffaloes in different lactations and effect of method of milking on teat condition, milk flow traits, udder health and milk composition was also studied. A comparative study on the milk yield and the maintenance behaviour was also studied in two different housing systems viz., conventional tie stall and loose housing. The results showed that among the different udder shapes, the bowl shapes were very common followed by globular, pendulous and goaty shapes and their occurrences were 61.0 %, 17.0 %, 13.0 % and 9.0 %, respectively, and the occurrence of cylindrical teats was maximum with a percentage of 52.5 followed by pear, bottle, conical and funnel type of teats with 18.0, 11.0, 10.5 and 8.0% respectively. The mean length, width and depth of the udder in different lactations ranged from 52.21 ± 0.61 to 55.71 ± 0.59, 48.61 ± 0.60 to 53.87 ± 0.70 and 15.46 ± 0.17 to 15.78 ± 0.18 cm, respectively. Positive and significant correlations were observed among all the udder measurements. The average length of left fore, right fore, left hind and right hind teats were 7.37 ± 0.15, 7.53 ± 0.16, 8.23 ± 0.18 and 8.12 ± 0.17 cm, respectively, and the mean diameters in the same order were 2.65 ± 0.02, 2.71 ± 0.03, 2.80 ± 0.03 and 2.89 ± 0.04 cm. The average daily milk yield in Murrah buffaloes with various udder shapes observed was 6.41 ± 0.33, 5.91 ± 0.26, 5.61 ± 0.32 and 6.31 ± 0.16 Kg in bowl, globular, goaty and pendulous type of udders, respectively. It was found that the average daily milk yield in buffaloes with cylindrical shaped teats was highest followed by bottle, funnel, pear and conical shaped teats. Out of the total buffaloes, the occurrence of different temperaments were 53.5, 16.0, 15.5, 9.5 and 5.5 percent for docile, slightly restless, restless, aggressive and nervous temperaments, respectively and neither the udder shape nor the teat shape affected the temperament of the buffaloes. It was observed that the restless and slightly restless buffaloes were more frequent in primiparous buffaloes (35.18 and 33.33 %, respectively) followed by docile (24.07 %), aggressive and nervous type (3.7% each). In the buffaloes under second, third and fourth lactations, docile buffaloes were more frequent (73.33, 57.40 and 59.37 %, respectively) when compared to other temperaments. It was observed that kicking (16.4 %), urination (4.8 %) and vocalization (4.4 %) were more frequent in machine milked buffaloes where as the stepping (46.8%) and defecation (2.4%) were more frequent in hand milked buffaloes. However, forceful throwing of head behaviour was equally frequent in both methods of milking (12 %). The machine milked buffaloes had a better teat condition score in the present study. The percentage of machine milked buffaloes with the teat condition of smooth skin (1.0), slight drying of the skin (1.5), dried skin (2.0), dry skin with cracks (2.5) and cracks with chaps (3.0) were 34, 17, 30, 13 and 6 %, respectively, whereas the number of buffaloes in the same order under hand milking were16, 2, 52, 27 and 3 %, respectively. The average evening milk yield of buffaloes under machine and hand milking was 3.62 ± 0.08 kg and 3.51± 0.08 kg, respectively. The mean percentages of milk fat, SNF and total solids under machine and hand milking were 6.01± 0.08 vs 6.57 ± 0.09, 9.19 ± 0.10 vs 8.79 ± 0.10 and 14.80 ± 0.13 vs 15.36 ± 0.13 %, respectively. The stripping time and the total milking time were higher in hand milking while the strip yield and average flow rate were higher in machine milking. In the present study, it was found that there was no significant difference in the somatic cell count of buffalo milk under machine and hand milking. The milk yield in the present study was higher in loose housing (8.12 ± 0.02 Kg) when compared to conventional housing (7.77 ± 0.02 Kg). The cleanliness score was higher in loose housing system (2.80 ± 0.05) compared to that of conventional housing (2.41 ± 0.05). More number of buffaloes had shown the lameness in the conventional system of housing. Out of 12 buffaloes observed for behavioural patterns, 36.48, 62.95, 44.90, 25.05 and 26.10 percent were found lying, standing, feeding idling and ruminating, respectively under conventional housing system while 47.29, 50.24, 37.91, 36.29 and 26.10 percent were observed for same behavioural patterns under loose housing system and the differences between the two housing systems were found to be statistically significant for lying, standing and idling behaviours.ThesisItem Open Access ADVANCED STUDIES ON DIFFERENTIAL DIAGNOSIS AND THERAPEUTIC MANAGEMENT OF VOMITING IN DOGS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-05) SURESH, KAMINENI; ALAHA SINGARI, N(MAJOR); NALINI KUMARI, K; SURESH KUMAR, R.V; ESWARA PRASAD, PABSTRACT : The present study was undertaken to study the prevalence of vomiting, its causative factors, other associated symptoms, haemato biochemical changes, ultrasonographic findings, endoscopic appearance and efficacy of antiemetic drugs in afebrile dogs with vomiting. A total 135 (8.67%) afebrile vomiting dogs out of 1558 were selected during the study period. Vomiting was mostly observed in Pomeranian breed and the prevalence was higher in male pups below 6 months of age. Dietary abnormalities were found to be the causative factors for vomiting in majority (45.93%) cases followed by renal failure, hepatic disorders, gastric ulcers, parasitic infection, foreign bodies, pyometra, Diabetes ketoacidosis and tumors. Apart from vomiting anorexia, depression to lethargy, loss of weight and dehydration of varying degrees were the clinical signs evident in all dogs. In addition haematemesis and anaemia in renal failure, hepatic disorders and gastric ulcers; abdominal pain due to the presence of foreign bodies and tumors; polyuria and oliguria in renal failure; emaciation, icterus, polydipsia and ascitis in hepatic disorders; diarrhoea, melena and anaemia in parasitic infection and polydipsia, polyuria, polyphagia and cataract in Diabetes ketoacidosis were the other characteristic signs. High PCV, Hb and TEC counts in dietary abnormalities, foreign bodies and Diabetic ketoacidosis; increased TLC in renal failure, hepatic disorders, gastric ulcers, pyometra and tumors; increase in neutrophils in renal failure, hepatic disorders, gastric ulcers, pyometra and tumors and high eosinophil count in parasitic infestation and gastric ulcers were prominent in dogs with vomiting. Hyponatremia due to presence of foreign bodies and Diabetic ketoacidosis; hypokalemia and hypochloremia in all cases; increased ALT levels in hepatic disorders; hypoproteinemia and hypoalbuminemia in hepatic disorders, gastric ulcers and tumors; high globulin levels in pyometra; hyperglycemia in Diabetic ketoacidosis and elevated BUN and creatinine levels in renal failure were the characteristic biochemical alterations noted in dogs with vomiting. Gas filled intestines with plication in gastro enteritis, prominent kidney opacity in renal failure; ground glass appearance in ascites; foreign bodies in the oesophagus, stomach and intestines; uterine distension in pyometra and intra abdominal neoplasm were the radiographic abnormalities detected in the present study. Generalized mucosal oedema along with foam and several streaks of bile tinged fluid in most cases of gastritis along with haemorrhages, erosions, large ulcers, shallow ulcers and hyperemic gastric mucosa were revealed on endoscopy. Hyperechoic cortex, loss of detail of CMJ, hyperechoic medulla and thickened urinary bladder wall in renal failure; hyperechogenecity, hepatomegaly, anechoic ascitic fluid and cholecystitis in hepatic disorders and enlargement of uterus and uterine horns in pyometra were the abnormalities recorded on ultrasonography. In the therapeutic trial conducted as a part of present study 3 (I, II and III) groups of dogs with vomiting due to dietary abnormalities and 2 (IV and V) groups with renal failure were selected with 12 dogs in each. Ringers lactate was given twice daily and Vitamin B1, B6 and B12 once in a day for all dogs. Metoclopramide (Group I), Ondansetron (Group II) and Ondansetron and potassium chloride twice daily (Group III), Metoclopramide and Amoxicillin and cloxacillin (Group IV) and Ondansetron and Amoxicillin and cloxacillin (Group V) were the drugs employed. Though there was significant improvement in all the groups, based on the faster resolution of vomiting and improvement in the haemato biochemical parameters, treatment with Ondansetron and potassium chloride was found to be superior when compared to other two combinations in vomiting due to dietary abnormalities and Ondansetron was found to be superior when compared to Metoclopramide in controlling vomiting due to renal failure.ThesisItem Open Access ADDITION OF A HERB TO A STATIN: A POSITIVE OR NEGATIVE INTERACTION? EXPERIMENTAL STUDIES ON DYSLIPIDAEMIAS IN RATS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-03) DILIP REDDY, GUNTURU; GOPALA REDDY, A(MAJOR); SRINIVASA RAO, G; ANAND KUMAR, A; RAJASEKHAR REDDY, AABSTRACT : A total of 56 male Sprague dawley rats of uniform weight and age were randomly divided into seven groups consisting of eight rats in each group after an acclimatization period of 3 weeks to evaluate the interaction of atorvastatin with garlic in induced dyslipidaemia. Group 1 served as plain control, while groups 2 and 3 were fed with high fat and high cholesterol diet throughout the experimental period. Groups 4,5,6 and 7 received 1% (100% dose), 0.5% (50% dose), 0.25% (25% dose) and 0.75% (75% dose) fresh garlic w/w in feed, respectively in addition to the high fat and high cholesterol diet and administered with 10 (100% dose), 5 (100% dose), 7.5 (100% dose) and 2.5 (25% dose) mg/kg atorvastatin respectively, while group 3 served as atorvastatin control, which received 10 mg/kg atorvastatin per day orally for 12 weeks. Blood collection was carried out at every two weeks interval for plasma biochemical analysis of total cholesterol, HDL cholesterol, triglycerides and creatinine and aspartate transaminase (AST). Single dose and multiple dose pharmacokinetic studies were performed at the beginning of the first dose and at the end of last dose of atorvastatin, respectively in groups 3 to 7. At the end of the experiment, liver and kidneys were collected for assay of TBARS, glutathione and SOD. Histological, histochemistry and electron microscopy studies were conducted on different organs at the end. All the treatment groups exhibited significant improvement in dyslipidaemic condition when compared with group 2 from 2nd week of treatment by reducing the TC, TG and LDL-C levels with subsequent increase in HDL-C levels. Group 4 was highly effective in correcting dyslipidaemia due to the synergistic pharmacodynamic actions of herb and drug. Plasma atorvastatin concentrations during multiple dose PK studies were significantly higher than single dose counterparts. PK parameters showed a significant increase in the garlic treated groups with high values of Cmax, AUC, AUMC, MRT and half-life which could be attributed to the inhibitory activity of garlic on drug transporters and metabolizing enzymes. High concentration of the drug in plasma in group 4, 5 and 3 resulted in toxicological manifestations in liver and kidney, which was evident from the increased plasma creatinine concentration, AST activity and oxidative stress. Histopathological studies on liver, kidney revealed moderate to severe damage in groups 4 and 5, which also exhibited mitochondrial damage on transmission electron microscopy. From this study, it can be concluded that garlic and atorvastatin exhibited positive pharmacodynamic interaction in reducing dyslipidaemias. The pharmacokinetic studies revealed that garlic increased the pharmacokinetic parameters and the toxicological studies indicated that high dose of atorvastatin + garlic has negative safety profile. Further studies are warranted to address the pharmacokinetic interactions of statin and garlic in detail.ThesisItem Open Access STUDIES ON CARDIAC DISEASES AND THERAPEUTIC MANAGEMENT OF CONGESTIVE HEART FAILURE IN DOGS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2009-01) SATlSH KUMAR, K; ALAHA SINGARI, N(MAJOR); TIRUMALA RAO, D.S; RAGHAVENDER, K.B.P.; ESWARA PRASAD, P; VYKUNTA RAO, VA RSTRACT: The present investigation was undertaken to study the occurrence of cardiac diseases, and the efficacy of different therapeutic regimens of congestive heart failure in dogs Dogs presented to \,'eterinary Hospital. Bhoiguda Teaching L'eterinary Clinical Complex. College of Veterinary science, Hyderabad from October 2006 to November 2008 i e , for a period of 26 months were considered for the study During this period 27,618 dogs were presented to the Hospital. ot' which 886 were suspected to ha~eca rdiac disease based on the clinical man~festat~crnasn d 4-71 Mere diagnosed with various cardiac diseases based on clinical examination, haematology. biochemistry. electrocardiography. radiography and echocardiography The owrall occurrence of cardiac disease in doss was 1 65 OO and the occurrence of the disease in the population exhibiting clinical manifestations was 48 b '0 The h~shesr prevalence was recorded in Labrador Retriever (13 26 ''0) folloued by Spitr German Shepherd, Doberman Pincher. Great Dane, Nondescript breed, Boxer, Cmlden Retriever. Rot\veiler and lowest in Dachshund ( 1 97 Oo) The age wise occurrence of cardlac d15eas in dogs was highest (61.13 O.0) in between 10 to I4 years of age and In\\est (5 It, in do@ with less than 5 years of a_re The scx wise occurrence of cardiac disease in dogs \\as bQ 10° o in males and 30 600,,0 in females The clinical manifestations recorded in the present investigation were exercise intolerance in 282 (65.47 %), dyspnoea at rest in 264 (61.25 %), anorexia in 249 (57 68 %), cough in 185 (42.85 Oh), going down in condition in 169 (39 14 Oh), pale mucosa in 11 5 (26.65 %), ascites in 49 (1 1.26 %), peripheral edema in 34 (7 80 Oh), cyanosis in 24 (5.64 Oh) and syncope in 10 (2.35 %) dogs and murmurs, gallop sounds and arrhythmias in all the dogs According to ISACHC regulations, out of 43 1 cases, 118 (27.3 %) dogs were found to be under class 11, 294 (68 2 Oh) dogs were under class 111 A and 19 (4 5 ?/o) animals were in class 111 B and 29 (6 68 ?/o) dogs belonged to phase 11, 58 (13.5 YOb) elonged to phase I11 and 344 (79.82 %) were under phase IV as per New York Heart Association recommendations The mean values of hemoglobin and total erythrocyte wunt was significantly low with an increase in TLC levels of all the CHF dogs on day 0 before therapy when compared with healthy dogs. The mean CKMB. LDH and Na levels were significantly elevated with a decrease in TP and serum albumin levels of all the CHF dogs before therapy The various ECG abnormalities detected in 384 dogs out of 431 dops with cardiac diseases \vere increased R amplitude m.ith ST slurring (8 6 Oh). Deep S na\.e (6 il OOJ. Increased P and R wave amplitudes (6 O OW), Deep Q and elevated R wave (6 0 40), abnormal confiyuration of P wave (5.72 9b). deep Q wave (2.21 O.b), wide P wave (4 94 Ob), low voltage CJRS complexes (1 95 O 0). \.entricular tachycardia (4 69 O 0). absence of P na\c (3 16 OO). elevated R Ma\ e. wide QRS. ST co\.ing (3 9 1 ). configurational change in P \\a\ e- \r ondering sinus pacemaker (-3 h5 OO), increased amplitude and width of P wave (3 -39 '0). ventricular premature complexes with atrial enlargement (3 39 96). elevated T wave (3 39 '0). fine atrial fibrillation (3 -39 OO). junctional premature conlplexes \\.ith 11 degree .A\' block (2 86 '0). ventricular premature complexes of riyht ventricle origin ( 2 86 OO). hrad\cardia \cith low voltage QRS complexes,. notched R wave (3 60 OO), ventricular bigeminy of left ventricle origin (2 60 O,O). deep T wave (7 60 96). sinus arrhythymia m.ith low R wave (2 34 '0). bundle branch block (7 34 O 0). reg~llar\ ,entricular premature complex -- after e\ erv second beat (-1 08 ?b) and \.cntricular depolarization from ectopic foci of hean. nler3ing uith riorrnal sinus rhythm - A complex arrhythmia ( 1 82 O 0) On radlopraphy. 58 (38 88O0) dogs showed pulmonw benous congestion. 49 (32 hhOo) showed cardlomegaly. Ih ( 17 ;3 '0) an~n~aslhso med aurlculilr hulsr leti dtrlal enlargement and 17 ( 1 1 33O 0) dops revealed pleural and perlcardlal ctTuslon In the present study out of a total of 150 dogs subjected to echocardiography, dilated cardiomyopathy in 74 (49.33 %) dogs, mitral valve insufliciency in 88 (58.66 %), tricuspid valve insufliciency in 37 (24.66 %), pericardial efision in 28 (18.66 %), hypertrophic cardiomyopathy in 8 (5.33 %) and patent ductus aneriosus in 5 (3.33 %) dogs was revealed. The LVEdD, LVEsD, and EPSS dimensions were increased with a decrease in LVPWd, LVPWs. IVSd, IVSs, and contractility indices (EF and FS) of DCM associated CHF dogs. There was a significant turbulence on pulsed wave Doppler and mosaic panern appearance on color flow Doppler of the mitral valve in MVI associated CHI- dogs In the present study all the 43 1 dogs diagnosed for heart disease were negative for the presence of adult heanworm antigen in the blood Out of the total patients diagnosed for various cardiac diseases. 60 patients diagnosed to be ailing ivith Congestive Heart Failure (30 dogs with DCM and 30 u.ith lLlitral Valve Insutliciencv) based on hemato-biochemistry. ECG, radiogaphy and echocardiography were selected and subjected to different therapeutic regimens. The selected 60 patients were randomly di\ ided into 3 groups, Cir 1. II and 111 each having 20 (10 DC\I and 10 XfVi) dogs Dogs in group 1 were treattxi with benazapril. frusemide and digovin once dad!.. orall!- Dogs in group 11 were treated with ramipril, fmsemide and digoxin once daily, orally Dogs in group I11 were treated uith ramipril. frusemide and digoxin at the same dosage as given to the dogs of the group I1 and a neutraceuticle. L-C'arnitine for L>C\1 dogs and Coenzyme Q10 fix mitral \ al\ e insufficiency doss .All the dogs under treatment for 45 da!.s \\ere monitored and the eflicacy was assessed at tbrtniyht intewals based on improvement of' clinical signs. henlato-biochemistry. electrocardiography. radiography and echocardiography features. 1-here \\as a significant impro\ement in the haen~atobinchemical parameters of CHF dogs during therap!. Radiographicall?. no appreciable reduction in heart size uas obseried in any of the Cl1F dogs after 45 days of therapy except substantial reduction in the pulmonarv edenla and pleural effusion There was improvement and disappearance in the cardiac arrh17hnlias on electrocardiograph^ A significant ditkrerice in left \.entncle dinlensions (LVEdD. L\'LisD. l.\'PM'd. I \'PWs. I\.Sd. IVSs. and EPSSl and contractilit! indices (EF and FS) were noticed tn W'hl associated CHF dogs b! the end of the trial Whereas. nu spmific difference in severity and intensity of turbulence and mosaic pattern of cvlour jet flow at m~tral \,a!\ e was noticed in (1'1 IF dogs associated with J2\-1 All the CHF dogs of group 111 recovered by the end of therapeutic trial Whereas, 5 dogs in group 1 and 3 in grwp 11 showed signs of azotemia and died after five weeks in spite of reduction in the doses of digoxin and ACEi Based on the resolution of clinical signs, improvement in haemato biochemical parameters and echocardiographic features, it was found that a combination of ramipril, frusemide and digoxin along with neutriceutical supplementation of L-Carnitine and Coenzyme Q was superior to other two combinations as indicated by the faster disappearance of sips and early recovery with no adverse drug reactions both during and after therapy Hence. this therapeutic regimen is indicated in dogs with congestive heart failure associated w~th either dilated cardiomyopathy or mitral valve insufficiency.