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  • ThesisItemOpen Access
    EFFICACY OF PROSTAGLANDIN INDUCED MILK FLOW TEST (PGIMFT) FOR EARLY PREGNANCY DIAGNOSIS IN ONGOLE (Bos indicus) COWS
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P) INDIA, 2015-10) SATISH, SANAPATI; MUTHA RAO, M(MAJOR); VENKATA NAIDU, G; DEVI PRASAD, V
    ABSTRACT: The aim of this research was to determine the accuracy and efficacy of PGIMFT for early diagnosis of pregnancy in Ongole cows. The diagnosis of pregnancy was based on observation of milk ejection response due to release of luteal oxytocin induced by intravenous administration of non-luteolytic dose of PGF2α. The study was performed on 42 lactating cows, 22-23 days post insemination, 5 hours after morning milking. The cows were divided into 3 groups; 250μg of PGF2α (Group I, n=16) and 500μg of PGF2α (Group II, n=16) were administered intrajugularly, while group III cows served as control. The pregnancy status was confirmed by per rectal examination 45-60 days post AI. It was found that the accuracy of the test did not vary between the groups. However, the accuracy of positive result (90.47 vs. 84.61%), negative result (45.45 vs. 25.0%) and overall diagnostic accuracy (75.0 vs. 61.90%) non-significantly exceeded that obtained by ELISA of plasma progesterone method. The test being inexpensive, rapid, easy to interpret, field friendly and does not require highly qualified personnel to perform, can be used for early pregnancy diagnosis with reasonable accuracy
  • ThesisItemOpen Access
    EFFICACY OF PROSTAGLANDIN INDUCED MILK FLOW TEST (PGIMFT) FOR EARLY PREGNANCY DIAGNOSIS IN ONGOLE (Bos indicus) COWS
    (SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502 (A.P) INDIA., 2015-10) SATISH, SANAPATI; MUTHA RAO, M (Major); VENKATA NAIDU, G; DEVI PRASAD, V
    ABSTRACT : The aim of this research was to determine the accuracy and efficacy of PGIMFT for early diagnosis of pregnancy in Ongole cows. The diagnosis of pregnancy was based on observation of milk ejection response due to release of luteal oxytocin induced by intravenous administration of non-luteolytic dose of PGF2α. The study was performed on 42 lactating cows, 22-23 days post insemination, 5 hours after morning milking. The cows were divided into 3 groups; 250μg of PGF2α (Group I, n=16) and 500μg of PGF2α (Group II, n=16) were administered intrajugularly, while group III cows served as control. The pregnancy status was confirmed by per rectal examination 45-60 days post AI. It was found that the accuracy of the test did not vary between the groups. However, the accuracy of positive result (90.47 vs. 84.61%), negative result (45.45 vs. 25.0%) and overall diagnostic accuracy (75.0 vs. 61.90%) non-significantly exceeded that obtained by ELISA of plasma progesterone method. The test being inexpensive, rapid, easy to interpret, field friendly and does not require highly qualified personnel to perform, can be used for early pregnancy diagnosis with reasonable accuracy.