EFFICACY OF PROSTAGLANDIN INDUCED MILK FLOW TEST (PGIMFT) FOR EARLY PREGNANCY DIAGNOSIS IN ONGOLE (Bos indicus) COWS
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Date
2015-10
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SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502 (A.P) INDIA.
Abstract
ABSTRACT :
The aim of this research was to determine the accuracy and efficacy of PGIMFT
for early diagnosis of pregnancy in Ongole cows. The diagnosis of pregnancy was based
on observation of milk ejection response due to release of luteal oxytocin induced by
intravenous administration of non-luteolytic dose of PGF2α. The study was performed
on 42 lactating cows, 22-23 days post insemination, 5 hours after morning milking. The
cows were divided into 3 groups; 250μg of PGF2α (Group I, n=16) and 500μg of PGF2α
(Group II, n=16) were administered intrajugularly, while group III cows served as
control. The pregnancy status was confirmed by per rectal examination 45-60 days post
AI. It was found that the accuracy of the test did not vary between the groups. However,
the accuracy of positive result (90.47 vs. 84.61%), negative result (45.45 vs. 25.0%) and
overall diagnostic accuracy (75.0 vs. 61.90%) non-significantly exceeded that obtained
by ELISA of plasma progesterone method. The test being inexpensive, rapid, easy to
interpret, field friendly and does not require highly qualified personnel to perform, can
be used for early pregnancy diagnosis with reasonable accuracy.
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Keywords
PROSTAGLANDIN INDUCED MILK FLOW TEST; PGIMFT; ONGOLE COWS; Bos indicus; ; EARLY PREGNANCY