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Author: BALA KRISHNA RAO, M.V. × End date: 2019 ×
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    ThesisItemOpen Access
    EFFICACY OF PROSTAGLANDIN INDUCED MILK FLOW TEST (PGIMFT) FOR EARLY PREGNANCY DIAGNOSIS IN BUFFALOES (Bubalus bubalis)
    (SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2017-06) BALA KRISHNA RAO, M.V.; MUTHA RAO, M(MAJOR); VENKATA NAIDU, G; HARI KRISHNA, N.V.V.
    ABSTRACT: The aim of this research was to determine the accuracy and efficacy of PGIMFT for early pregnancy diagnosis in buffaloes. The diagnosis was based on observation of milk ejection response due to release of luteal oxytocin induced by intravenous administration of non-luteolytic dose of PGF2α. The study was performed on 80 lactating Graded Murrah buffaloes, 22-23 days post insemination, 5 hours after morning milking. The buffaloes were divided into 3 groups; 125 μg of PGF2α (Group I, n=36), 250 μg of PGF2α (Group II, n=36) were administered intrajugularly and Group III buffaloes (n=8) served as control. The pregnancy status was confirmed by per rectal examination at 45-60 days post AI. It was found that the accuracy of test did not vary between the groups. However, the accuracy of positive result (63.41 vs 64.70 %), negative result (61.29 vs 50.00 %) and overall diagnostic accuracy (62.50 vs 56.25 %) non-significantly exceeded that obtained by ELISA of plasma progesterone method. The test being inexpensive, rapid, easy to interpret, user friendly at field level and does not require highly skilled personnel to perform, could be used for early pregnancy diagnosis in buffaloes with reasonable accuracy