EFFICACY OF PROSTAGLANDIN INDUCED MILK FLOW TEST (PGIMFT) FOR EARLY PREGNANCY DIAGNOSIS IN BUFFALOES (Bubalus bubalis)
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Date
2017-06
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The aim of this research was to determine the accuracy and efficacy of PGIMFT for
early pregnancy diagnosis in buffaloes. The diagnosis was based on observation of milk
ejection response due to release of luteal oxytocin induced by intravenous administration of
non-luteolytic dose of PGF2α. The study was performed on 80 lactating Graded Murrah
buffaloes, 22-23 days post insemination, 5 hours after morning milking. The buffaloes were
divided into 3 groups; 125 μg of PGF2α (Group I, n=36), 250 μg of PGF2α (Group II, n=36)
were administered intrajugularly and Group III buffaloes (n=8) served as control. The
pregnancy status was confirmed by per rectal examination at 45-60 days post AI. It was
found that the accuracy of test did not vary between the groups. However, the accuracy of
positive result (63.41 vs 64.70 %), negative result (61.29 vs 50.00 %) and overall
diagnostic accuracy (62.50 vs 56.25 %) non-significantly exceeded that obtained by ELISA
of plasma progesterone method. The test being inexpensive, rapid, easy to interpret, user
friendly at field level and does not require highly skilled personnel to perform, could be
used for early pregnancy diagnosis in buffaloes with reasonable accuracy
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