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ThesisItem Open Access CLINICAL- HAEMATOBIOCHEMICAL AND THERAPEUTIC STUDIES ON CANINE ANEMIA(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA, 2017-12) Vijaya Bhaskar, D.P; Syaama Sundar, N (MAJOR); Vaikunta Rao, V; Rani Prameela, DAbstract: The study entitled “Clinical, Haemato–Biochemical and Therapeutic studies on Canine Anemiaʺ was carried out in the Department of Veterinary Medicine, Small animal ward, Veterinary Clinical Complex (VCC) N.T.R College of Veterinary Science, Gannavaram and NTR Super Speciality Veterinary Hospital (SSVH) Vijayawada, during the period of December 2017 to July 2018. During this period 1203 dogs were screened an 380 of them were found anemic. Among them 48 anemic dogs (24 Mild anemic dogs and 24 moderate anemic dogs) were selected randomly for clinical study. The present study revealed prevalence of anemia accounted 31.59%. The gender wise prevalence is higher in male dogs rather than female dogs. Age wise prevalence of canine anemia has noticed highest prevalence at the age group of 6 months to 4 years (31.32%) and breed wise highest prevalence recorded in Germen shepherd (23.42%) followed by Labrador (21.84%). In present study etiology of anemia revealed highest prevalence due to A. caninum (18.95%), followed by tick infestation (15.26%), haemorrahagic gastro entiritis ( 12.11%), hepatic disorders (11.05%), peduculosis ( 7.37%) , parvo viral infection ( 7.37%), Renal Disorders (7.11), Demodectic mange ( 6.05%), Gastro entiritis ( 5.53%), Sarcoptic mange (5.00%), Toxocara infection ( 2.11%), Leptospirosis (1.05%), Babesiosis ( 0.79%) and lowest prevalence was noted in Hepatozoon infection (0.26%). Out of 48 cases 81.25% dogs showed hypothermia, and 18.75% dogs showed hyperthermia, tachycardia (72.92%), tachypnoea (60.42%), bradycardia (27.08%), bradypnoea (39.58%) were recorded in anemic dogs. Dogs showed pale mucous membrane (64.58%), congested mucous membrane (29.17%) and icteric mucous membranes (6.25%) were recorded. The pale mucous membrane was recorded as a common clinical sign in the most of the cases. In mild anemic dogs body condition score (BCS) recorded in 54.17% of dogs were ideal weight, where as moderate anemic dogs 37.50% were under weight. Majority of mild anemic dogs has normocytic and normochromic anemia where as moderate anemic dogs has normocytic hypochromic anemia. Therapeutic regimen of anemic dogs were further grouped as Group –I: (n=12) mild anemic dogs treated with oral allopathic haematinic Group – II: (n=12) mild anemic dogs treated with oral ayurvedic haematinic. Group –III: (n=12) moderate anemic dogs treated with parenteral iron dextran (10 mg/kgbwt) once in a week along with allopathic haematinic. Group – IV: (n=12) moderate anemic dogs treated with parenteral iron dextran (10 /kgbwt) once in a week along with oral ayurvedic haematinic. The results reveals allopathic and ayurvedic medication has almost similar efficacy, comparatively ayurvedic drug treated dogs has shown less side effects like constipation compared to allopathic medication. Whereas moderate anemic dogs will give fast recovery with parenteral low molecular iron dextran along with either allopathic or ayurvedic haematinics.ThesisItem Open Access STUDIES ON CANINE MALASSEZIAL INFECTIONS AND ITS THERAPY(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2006-10) VENKATRAMAIAH, A; NALINI KUMARI, K(MAJOR); ALAHA SINGARI, N; Venkata Reddy, TABSTRACT : The incidence of Malassezial infections in three veterinary hospitals in Andhra Pradesh was studied during a total period of seven months. An over all incidence rates of 80.82% of Malassezia associated otitis and 7.14% of Malassezia dermatitis were recorded during the study period. The incidence of Malassezia associated otitis was 83% in Tirupati, 82% in Kakinada and 60% in Vijayawada. The incidence of Malassezia dermatitis was 11.90% in Tirupati and 10.45% in Kakinada and there was significant difference (P<0.01) between the incidence of Malassezia associated otitis and dermatitis. It was observed that Malassezial infection in the form of otitis was more common than in the form of dermatitis. Age wise incidence of Malasseziosis revealed that dogs upto twenty four months age group were more prone to Malassezia associated otitis and dermatitis. Sex wise analysis revealed that gender has got no significant influence on the incidence of Malassezial infections. Breeds of Dachshund, Labrador, Lhasa Apso, German shepherd and Pomeranians were more prone to Malassezial infections. Symptoms such as ear discharges of purulent or waxy nature, bad odour, head shaking, ear scratching, pain on palpation of ear, matting of hair intruding the ear canal and hair present at the base of the pinna and scales on the pinna were seen in Malassezia associated otitis. Alopecia, pruritus and erythema over ventral abdomen, neck, medial aspects of fore legs and mandibular region, poor hair coat, lichenification of ear were seen in Malassezia dermatitis. Roll smear cytology was made use of in the diagnosis of Malassezia associated otitis while tape impression smear for Malassezia dermatitis. In cases selected for therapy, Malassezia were seen in association with bacteria and neutrophils in eight cases and with only bacteria in eight more cases. Tape impression smears from dermatological cases revealed only Malassezia yeasts. Antifungal and antibiotic sensitivity tests were carried to all the otitis cases selected for therapy as bacteria was observed in all these cases along with the yeast organisms. Only antifungal sensitivity test was conducted in dermatological cases as only yeast but not bacteria could be found in tape impression smears. All the samples from Malassezia associated otitis were sensitive to Ketoconazole, Fluconazole, Miconazole, Itraconazole, Clotrimazole, Amphotericin-B and Nystatin. All samples (100%) from dermatological cases were sensitive to Ketoconazole and Clotrimazole, 75% were sensitive to Fluconazole, Itraconazole and Nystatin and 62.50% to Amphotericin-B. Antibiotic sensitivity test results of otitis cases revealed that all the samples were sensitive to Enrofloxacin, 88% showed sensitivity to Ciprofloxin and Gentamicin, 75% to Cephalexin and Cephadroxil, 69% to Chloramphenicol and 62.5% to Amoxycillin-clavulanic acid. Dogs with Malassezia associated otitis were divided into two groups (I and II) with eight cases in each of them. Similarly, eight cases of Malassezia dermatitis (Group III) were treated separately. Therapy of Malassezia associated otitis was carried with Clotrimazole ear drops, Ketoconazole shampoo bath and oral Enrofloxacin in group I and Clotrimazole ear drops, Selenium sulfide shampoo bath and oral Enrofloxacin in Group II. Dogs with Malassezia dermatitis were subjected to Miconazole shampoo therapy and in one case with lichenification, oral Ketoconazole was given besides Miconazole shampoo bath. It was observed that cases treated with Clotrimazole, Ketoconazole and Enrofloxacin recovered comparatively faster. Treatment with Miconazole shampoo was found to be successful in seven cases with mild symptoms of Malassezia dermatitis. However, one case with lichenification of ears responded well only after oral Ketoconazole therapy. It is concluded that Clotrimazole ear drops in combination with Ketoconazole was comparatively more efficacious in the treatment of Malassezia associated otitis. Mild cases of Malassezia dermatitis could be treated with Miconazole shampoo therapy.ThesisItem Open Access CLINICAL -BIOCHEMICAL AND THERAPEUTIC STUDIES ON POST-PARTURIENT ANOREXIASYNDROME IN BUFFALOES(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2005-08) MANIKYA REDDY, P; TIRUMALA RAO, D.S(MAJOR); PADMAJA, K; SADASIVARAO, KThesisItem Open Access STUDIES ON PERIODONTAL DISEASE AND THERAPEUTIC MANAGEMENT IN DOGS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2017-11) SHIREEN LAWRENCE, A; SURESH, K(MAJOR); NALINI KUMARI, K; VENU, RABSTRACT: The present study was undertaken to study the incidence of periodontal disease to carry out investigation on PD in dogs and to assess the therapeutic efficacy in stages 1 and 2 of periodontal diseases. The overall incidence was 86.67 percent. Breed wise incidence showed highest incidence of occurrence in boxer, irish setter, Chihuahua and shih tzu followed by Mongrel. Age wise incidence was highest in age group of 9 years and above with no sex predisposition. Small sized dogs and dogs fed with soft diets had higher incidence of periodontal diseases. Clinical signs in periodontal disease included plaque formation, gingivitis, halitosis, drooling of saliva, pawing the mouth, calculus deposition, gingival recession, gums that bleed when probed, discomfort on eating, tooth mobility, purulent exudates and ulceration of the gingival mucosa. Orastrip was quick check examination tool for assessing the oral health status and in the assessment of active periodontal disease. Staging of PD was done based on the periodontal indices namely attachment loss and furcation. Radiographic examination in periodontal disease revealed widening of periodontal ligament space, alveolar bone destruction in dogs affected with stage 3 and 4. Transillumination showed mild to severe form of gingivitis suggesting active periodontitis and can be taken as initial room examination procedure in dogs affected with PD. Haematology showed neutrophilic leukocytosis. Serum biochemical findings did not show any statistically significant in the mean levels of ALT, AST, ALP, total protein, albumin, glucose, BUN creatinine and CK-MB in dogs with periodontal disease. Therapeutic protocol adopted in periodontal disease included scaling, polishing followed by administration of chews or brushing in stage 1 dogs affected with periodontal disease while in stage 2 the protocol included scaling, polishing and instillation of doxycycline gel in the areas of attachment loss and furcation followed by administration of chews or brushing. Brushing daily was found to be superior when compared to dental chews in both stage 1 and Stage 2 periodontal disease.ThesisItem Open Access CLINICO DIAGNOSTIC AND THERAPEUTIC MANAGEMENT OF CERTAIN TUMOURS IN DOGS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2017-03) AMRUTH KUMAR, V.V.V; Nalini Kumari, K(MAJOR); Satish Kumar, K; Gireesh Kumar, V; Lakshman, MABSTRACT: During the present clinical study a total of 48,600 dogs presented to small animal medical outpatient ward were screened and out of them 3,240 were suspected for neoplasms and 458 dogs among them were diagnosed for certain malignant tumours. Highest incidence of tumours was observed in the age group of >5-10 years. Majority of the cases reported were of spitz breed followed by Labrador retrievers. Female dogs were more affected with various tumours than males. From among the 458 dogs which were diagnosed 118 dogs were TVT, Mammary tumours (102), Perianal tumours (41) and other skin tumours like mast cell tumours, basalcell tumours, squamous cell carcinoma of skin were (106) and miscellaneous tumours like lipoma (29), lymphoma (22) SCC of oral cavity (19), osteosarcoma (18) and TCC of bladder (3). Ten dogs which were reported at the hospital were taken up as apparently healthy dogs and grouped as Group I. The TVT affected dogs were grouped into Group II with 20 dogs and Group III with 20 dogs. The TVT dogs were showing clinical signs like bleeding or discharges from genitalia, masses protruding from the genitalia, licking of the genitalia, anorexia or inappetance, bleeding or discharges from the external genitalia, nodules or cauliflower like friable masses in the vulva or in the prepuce / caudal penis in males, licking of the external genitalia, ulceration of the tumour, popliteal and / or inguinal lymph node enlargement, anorexia and / or inappetance, dysuria and / or tenesmus and lung metastasis. The FNAC and histopathological studies revealed anisocytosis and anisokaryosis in round to oval shaped cells which were indicating malignancy; electron microscopically cells revealed vesicular cytoplasm, dense round shrunken mitochondria and dilatation of the cisterns of endoplasmic reticulum. Radiographic examination revealed lung metastasis masses in stage III and stage IV TVT tumours where as ultrasonography did not reveal any organ metastasis of abdomen whereas tumour mass was hyperechogenic. Hematological examination showed significant increase in neutrophils and decline in lymphocytes before treatment. The mean serum biochemistry parameters revealed significantly high mean ALT, AST, ALP and decreased serum protein when compared to the apparently healthy dogs. Therapeutic trial was conducted with Inj Vincristine in Group II dogs and Inj Doxorubicin in Group III dogs weekly once for four and five weeks respectively. Complete response of the tumour mass was observed by day 21 and day 28 in 17 and 18 dogs of Group II and Group III respectively. The other clinical signs also declined with the therapy in all the dogs which responded. Hematological examination revealed significant decline in mean Hb, PCV, TEC, TLC and neutrophil count but a significant increase of lymphocyte count was observed during and after therapy when compared to that of before therapy. The mean BUN, ALT, AST and ALP revealed significant increase whereas total protein showed significant decrease during and after treatment. Nausea, anorexia, vomiting, alopecia, anaemia and weight loss were the side effects noticed during treatment with Inj. Vincristine. Seven dogs showed mild side effects from day 14 which increased by day 21 (third dose) of therapy in 11dogs. Side effects included vomiting, diarrhoea, anorexia and alopecia during treatment with Inj. Doxorubicin. These signs started in 9 dogs from day 7 and aggravated in 14 dogs by day 21. But the severities of the side effects seen during Inj. Doxorubicin treatment were more when compared to Inj. Vincristine The cases were monitored for a period of six months for recurrence of any growths and the associated symptoms. Two dogs in Group II showed recurrence after 4 months. In two dogs of Group III recurrence was seen after 4 months in one dog and 6 months in the other dog. These dogs were subjected to surgical excision of tumour mass. The mammary tumour affected dogs were grouped into Group IV with 20 dogs and Group V with 20 dogs. The important clinical signs included palpable masses in mammary glands with greater percent of solitary nodular tumours than multiple masses, single gland was more affected than multiple glands, other signs observed were lymph node enlargement, ulceration of tumour skin, anorexia, weight loss and lethargy. The FNAC and histopathological studies revealed neoplastic cells of varied sizes and basophilic nucleoli which were indicating malignancy; histopathological examination revealed epitheloid cells of round to spindle shape, large nucleus Out of the 102 mammary tumours diagnosed 79 were simple malignant tumours, among them adenocarcinomas were 29, tubulo papillary adenocarcinoms were 22 dogs, ductular adeno carcinoma were 19 dogs and solid carcinomas were 9. Mixed mammary carcinomas were diagnosed in 28 dogs out of which Myxochondroadenocarcinoma and fibrosarcoma were 7, Myxolipoadeno carcinoma were 5 and liposarcoma were 4. Electron microscopy revealed abundant cells with epithelial morphology. Radiographic examination revealed lung metastasis masses in stage III and stage IV mammary tumours where as ultrasonography of tumour mass revealed anechoic fluid filled cystic collections, hyperechoic areas with diffuse margins and anechoic areas with hyper echoic margins and no abnormal echo pattern of the abdominal organs. In the present study the mammary tumour affected dogs revealed insignificant decrease of mean Hb, PCV, TEC, lymphocytes and platelets, whereas neutrophils showed insignificant elevation before treatment when compared to the apparently healthy dogs, the serum biochemistry parameters revealed insignificant decrease of BUN and insignificant increase of mean serum creatinine, ALT, AST, ALP and total protein before treatment. The mammary tumour affected dogs were grouped into Group IV with 20 dogs and Group V with 20 dogs and were subjected for chemotherapy. Group IV dogs were given Inj Vincristine weekly once for five weeks and Group V dogs were given Inj. Paclitaxel once every twenty one days for five times. Clinical improvement was seen in dogs with chemotherapy wherein 12, 14 dogs of Group IV, V responded for treatment with partial response of tumour respectively. The other clinical symptoms of mammary tumours also declined in the dog which responded to therapy. Haematological examination of Group IV dogs before, during and after therapy revealed that the mean Hb, PCV and TEC had significantly declined (P<0.05) from day 0 to day 35. Leucopenia was seen by day 21 which continued until day 35. Neutropenia was observed by day 21 until day 35. Lymphocytosis was seen during the course of therapy. Thrombocytopenia was observed during therapy when compared to before therapy. The haematological parameters of Group V dogs revealed significant decline in mean Hb, PCV, TEC, TLC and Neutropenia, thrombocytopenia and lymphocytosis was seen during the course of therapy from day 0 to day 105.The Group IV dogs showed increase of biochemical parameters when compared to pre-therapeutic means, whereas ALP showed significant increase and total protein showed significant decline post therapeutically. Nausea, anorexia, vomiting, anaemia, mild alopecia and weight loss were the side effects noticed during treatment with Inj. Vincristine. Three dogs showed side effects like nausea, in-appetence from day 14 which aggravated day 21. The side effects associated with Inj. Paclitaxel included vomiting, diarrhoea, anorexia and alopecia. These side effects started in 9 dogs from day 21 aggravated in 14 dogs by day 63. The side effects seen during Inj. Paclitaxel treatment were more severe when compared to Inj. Vincristine therapy. In Group IV out of the twelve dogs which showed partial response during chemotherapy, six showed recurrence after four months which were treated by surgical excision among which two dogs died within fifteen days. Three dogs of Group V showed recurrence of tumour growth in the fourth month (1 dog) and fifth month (2 dogs) after chemotherapy. These dogs were then treated by surgical excision out of which one died. The perianal tumour affected dogs were grouped into Group VI with 10 dogs and Group VII with 10 dogs. In the present study the dogs suffering with perianal tumours were showing clinical signs like masses in perianal region, dyschezia, tenesmus, constipation, discomfort, hematochezia, ulceration, licking, anorexia, weight loss, lethargy, polydipsia and polyuria. FNAC of the tumour revealed polygonal cells with pinkish blue cytoplasm and small nucleus with prominent nucleoli, histopathology showed rosette like cells with connective tissue fibres among the 41 dogs with perianal tumours 24 were hepatoid adenocarcinomas, 9 were myxosarcoma, 5 were SCC and 3 were fibrohemangiosarcoma.. Ultra structure revealed cells with numerous endoplasmic reticulum and prominent golgi apparatus. Ultrasonography showed low vascularisation of the tumour and no abdominal metastasis. Haematology revealed leucocytosis and neutrophilia when compared to the apparently healthy dogs. Serum biochemistry revealed insignificantly increased mean BUN, creatinine, ALT, AST, ALP and total protein. The perianal tumour affected dogs were grouped into Group VI with 10 dogs and Group VII with 10 dogs and were subjected for chemotherapy. Group VII dogs were given Inj Vincristine weekly once for five weeks and Group VII dogs were given Inj. Cisplatin once every week for five weeks. Clinical improvement was seen in 5 dogs out of ten dogs of Group VI with partial response of the tumour. Among the Group VII dogs 7 dogs showed reduction in tumour size by day 21 and partial response was seen by day 35. The other clinical signs alleviated in the dogs which responded for the therapy. The haematological parameters revealed that both the groups of dogs showed significant decline in mean Hb, PCV, TEC, TLC, neutrophils and platelet count and significant increase in lymphocyte count. The Group VI dogs showed highly significant increase in mean serum ALT and ALP during and after therapy whereas, Group VII dogs showed significant increase in mean BUN, creatinine, ALT, AST, and ALP during and after therapy. Nausea, anorexia, vomiting, anaemia, mild alopecia and weight loss were the side effects noticed during treatment with Inj. Vincristine. Three dogs showed side effects like nausea and in-appetence from day 14. The side effects of Inj. Platinex included profuse vomiting, diarrhoea and anorexia. Which started in 8 dogs from day 14 slowly and aggravated in these dogs by day 21. The side effects seen during Inj. Platinex treatment were more severe when compared to Inj. Vincristine therapy. In Group VI three dogs showed recurrence after three months which were treated by surgical excision among which two dogs died within ten days. Two dogs of Group VII showed recurrence in the fourth month (1 dog) and fifth month (1 dog) after chemotherapy. These dogs were treated by surgical excision of which one died.ThesisItem Open Access CLINICO DIAGNOSTIC AND THERAPEUTIC STUDIES ON CERTAIN ECTOPARASITIC SKIN DISEASES IN DOGS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2016-05) SIVA NAGARAJU, G; NALINI KUMARI, K(MAJOR); SURESH, K; VENU, RABSTRACT: The present study was carried out to study the incidence, different diagnostic aspects, and to evaluate the efficacy of different therapeutic agents in certain ectoparasitic skin diseases in dogs. The overall incidence of ectoparasitic skin disease was 75.55% (238/315) of which tick infestation, flea infestation, lice infestation, and mite infestation were 63.02 % (150/238), 11.34% (27 /238), 18.48% (44/238) and 19.76% (17/86), respectively. However, mixed infestations were seen in 22 out of 238 dogs. The highest incidence was recorded in age group of 2-4 years in tick infested dogs, 1-2 years in flea infested dogs, 2-4 years in lice infested dogs and less than one year in mite infested dogs. Tick, flea, lice and mite infestation were recorded high in male, mongrel dogs. Alopecia, erythema, erosions and pruritus (mild, moderate and severe) were the common clinical manifestations noticed in tick, flea, lice and mite infested dogs. In flea infested dogs, flea dirt was major clinical finding. Whereas, anemia, papular eruptions, hyperpigmentation which were mostly distributed at the dorsal aspect of lumbo-sacral area, caudomedial thighs and ventral abdomen were also recorded. Dogs with lice infestation along with above said signs showed anemia, restlessness, intense irritation (biting lice) and debilitation (sucking lice). Mite infested dogs revealed papules, pustules, scales, lichenification and hyperpigmentation. Whereas, generalized lesions were commonly observed than that of localized lesions. Diagnosis of ectoparasitic skin infestation was made by visual examination, palpation, combing, examination of skin scrapings, hairpluck, acetate tape impression smears and otic discharge which revealed presence of ticks (Rhipicephalus sanguineus), fleas (Ctenocephalides canis), lice (Heterodoxus spiniger and Linognathus setosus) and mites (Demodex canis and Demodex cornei). Though there was a significant decrease in the mean Hb, PCV and increase in eosinophils of the tick, fleas and lice infested dogs, it was not seen in all the dogs of either group. In mange, besides the significant anemic changes, a significant increase in TLC, neutrophil and lymphocyte count was also noted. The biochemical finding in dogs with ecto-parasitic skin disease was a significant decrease in the mean total serum protein. Fecal examination of these ectoparasitic infested dogs revealed Toxocara canis, Ancylostome eggs and tape worm segment in 11.76% of dogs. Forty dogs that diagnosed with ectoparasitic disease, were included in therapeutic studies viz., Group II, III, IV, V and VI with 8 in each. Ticks infested (GroupII) dogs treated with single application of fipronil spot-on 9.7% w/v showed improvement in erythema, pruritus, alopecia and all the dogs were completely free from ticks after 2 weeks of therapy. Whereas, Group III (tick infestation) dogs that were treated by applying scalibor protector band containing 4% deltamethrin showed complete relief by 4th week and the lesions subsided in all the dogs as the intensity of tick infestation decreased. After therapy there was a significant increase of hemoglobin, PCV, mean total protein and significant decrease in mean eosinophil count in both groups. However, based on period of recovery on comparison of two therapeutic protocols, fipronil spot-on was found to be more effective than scalibor protector band. In Group IV (flea infestation) dogs’ treatment was initiated with fipronil 0.25% w/v mechanical pump spray @ 3-6 ml/kg b.wt, with which all the dogs became flea free by 1st week. Group V (lice infestation) dogs were treated once with fipronil spot on 9.7%w/v. All 8(100%) and even these dogs were found to be free from infestation by 1st week. There was significant increase in the mean Hb, PCV, total serum protein and significant decrease in eosinophil count of both group IV and V dogs by the end of period of monitoring. Mite infested (group VI) dogs were treated with ivermectin tablets @ 300 μg per kg b.wt, orally once daily and external application of medicated shampoo (petben). However, in those cases which were complicated with pyoderma along with the above therapy an antibiotic (enrofloxacin @ 5 mg/kg b.wt) was also added. Ivermectin was continued till two negative skin scrapings were obtained at weekly intervals. By day 20, four dogs ( 2 localised and 2 generalised ) became mite free. While one dog became negative only by day 80 and one dog remained positive till the end of the study i.e day 90. After therapy the mean hemoglobin, PCV, lymphocyte count and mean total protein values slightly increased with significant decrease in TLC, neutrophil and eosinophil count. From the present study, it was concluded that the overall incidence of ectoparasitic skin disease was 75.55% with tick infestation being the most common. Erythema, alopecia and pruritus of varying intensity were the most commonly found symptoms in the affected dogs. The mean Hb, PCV, total serum protein decreased in the affected and improved by the end of the period of monitoring. Spot-on preparation was convenient to apply and brought 100% recovery in lice and tick infestation by weeks one and two respectively with no recurrence. Spray preparation was also easy to apply and brought about 100% recovery in flea infestation by 1st week with no recurrence. The recovery rate in demodicid dogs was 87.5% with a prolonged treatment period in generalized demodicosisThesisItem Open Access CLINICO-BIOCHEMICAL AND THERAPEUTIC STUDIES OF RUMINAL ACIDOSIS IN GOATS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2013-12) TAMBE SATISH VALMIK; PADMAJA, K(MAJOR); NAGARAJ, P; GOPALA REDDY, AABSTRACT:The present study was designed to investigate the clinico-biochemical parameters and response to therapeutic agents in clinical cases of goats suffering with ruminal acidosis. Thirty clinical cases of ruminal acidosis presented to the Teaching Veterinary Clinical Complex, Campus Veterinary Hospital, and Veterinary Ambulatory Clinic, Mylardevpally, College of Veterinary Science, Rajendranagar, Hyderabad with the history of accidental ingestion of carbohydrate rich diet, inappetance to anorexia, suspended rumination and clinical manifestations of distended rumen and diarrhoea were selected and were divided based on severity of ruminal acidosis into three different groups consisting of 10 goats in each. The group I (mild acidosis), group II (moderate acidosis), and group III (severe acidosis) goats were subjected to the therapeutic trials and compared against ten apparently healthy goats from the same area with similar husbandry practices which served as healthy control (Group IV). Clinical signs exhibited by mild acidotic goats (Group I) were inappetance, mild bloat, reduced rumen motility, reduced rumination, semisolid faeces, firm and doughy condition of rumen on palpation of left flank and dull sound on percussion of left flank. The signs observed in moderate acidotic goats (Group II) were anorexia, distended abdomen, absence of rumination, dull appearance, absence of ruminal motility, grinding of teeth, frequent bleating, thick nasal discharge, pasty faeces, dehydration, fluid flashing sound on percussion and gurgling sound on auscultation of rumen. Whereas in severe acidotic goats (Group III), the signs were observed anorexia, severe depression, sunken eyes, staggering gait, lameness, absence of rumination, ruminal motility absent, watery diarrhoea, dehydration, oligouria to anuria, dysponea, tachycardia, subnormal body temperature, sternal and lateral recumbency. Clinical signs after treatment in all affected goats disappeared. Ruminal acidotic goats had subnormal body temperature, heart and respiration rates and skin fold time in all affected groups of goats significantly (p<0.05) increased, ruminal motility significantly (p<0.05) decreased in group I and absent in group II and III, conjunctival mucous membrane was normal, slightly congested and congested in group I, group II and III respectively. There was no significant difference in the values of temperature, heart and respiration rates, skin fold time and ruminal motility in all affected groups of goats and conjunctival mucous membrane was normal after treatment. Rumen fluid samples were analysed for physical changes. Colour was gray in group I and milky gray in both group II and III, consistency was semisolid or slightly watery in group I and watery in both group II and III, odour was acidic in both group I and II and pungent acidic in group I, and significant (p<0.05) decrease in pH between the groups of affected goats before therapy. Micro-biochemical changes of rumen fluid like MBRT was significantly (p<0.05) increased in all affected groups of goats, SAT was significantly (p<0.05) increased in group I and absent in group II and III, iodophilic activity was reduced in group I and nil in both group II and III, protozoal motility was sluggish in group I and absent in both group II and III, protozoal density decreased in group I and absent in both group II and III, significant (p<0.05) decrease in live and increase in dead proportion in group I, complete absence of protozoa in both group II and III, and replacement of rumen fluid gram negative bacteria by gram positive bacteria in all affected groups of goats before treatment. Rumen fluid analysis was normal in all the groups after the treatment. In haematological parameters, a significant (p<0.05) increase in haemoglobin, packed cell volume, total leucocyte count, neutrophils, eosinophils, monocytes and significant (p<0.05) decrease in lymphocytes in all affected groups of goats before treatment. Haematological parameters were retured to normal in all the groups after the treatment. Blood biochemical analysis revealed significant (p<0.05) increase in plasma glucose, serum blood urea nitrogen and creatinine, serum aspartate aminotransferase and serum lactate dehydrogenase enzymes were observed in all affected groups of goats before treatment. After treatment all blood biochemical parameters returned to normalcy in all the groups after the treatment. The mean values of urine pH before treatment was significantly (P < 0.05) decreased between the all affected groups as compared with group IV. The mean values of urine pH did not differ significantly after treatment in group I, II and III. The Benedict’s test of urine was positive in all affected goats before treatment as compared to group IV. After therapy, it was negative in all ruminal acidosis affected goats. The therapeutic efficacy of the drugs used against various types of ruminal acidosis in goats was assessed. Group I goats were given sodium bicarbonate @ 1 g/kg body weight, single dose orally daily for 5 days. Group II goats were given ‘Bufzone’ @ 50 g single dose orally daily for 4 days. Whereas, goats of group III were administered ‘Bufzone’ @ 50 g single dose orally daily for 4 days and Sodium bicarbonate @ 1 g/kg body weight orally for 5 days. In addition goats of all the three groups were administered with Chlorphenarmine maleate @ 0.5 mg/kg body weight im, Tribivet @ 2ml im and Ringer’s lactate @ 25 ml, 50-75 ml, 75-74 100 ml iv in group I, II and III respectively. It was concluded that ruminal acidosis is a common disease of goats and its severity can be effectively reduced by combination of Bufzone and sodium bicarbonate along with supportive therapy.ThesisItem Open Access CLINICAL AND DIAGNOSTIC ASPECTS OF CERTAIN COMMON DERMATOSES IN DOGS WITH PARTICULAR REFERENCE TO CANINE ATOPIC DERMATITIS(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 2012-12) JYOTHI, JATAVATH; Satish Kumar, K(MAJOR); Nagaraj, P; Chandrashekhar Reddy, K; Madhava Rao, TABSTRACT : The present study was carried out in a total of 214 dogs that were presented with the history and signs of skin and coat abnormalities. These cases when subjected to thorough clinical examination and skin samples analysis, mange, atopy, malasseziosis, hypothyroidism, pyoderma, dermatophytosis and mixed infection associated dermatoses was recorded in 23.83 per cent, 21.50 per cent, 15.89 per cent, 12.62 per cent, 10.75 per cent, 8.41 per cent and 7.00 per cent dogs, respectively. The breed wise prevalence of mange was highest in GSD and lowest in Saint Bernard and that of canine atopic dermatitis was the highest in Dalmatian and lowest in Pug. Similarly, GSD and Doberman and Golden retriever; Labrador and Cocker spaniel, Golden retriever and Daschund; Spitz and Mongrel; GSD and Spitz and Doberman; GSD and Golden retriever were recorded as highest and lowest prevalence for malasseziosis, hypothyroidism, pyoderma, dermatophytosis and mixed infestation, respectively. Dermatoses associated with mange, atopic dermatitis, malasseziosis, hypothyroidism, Pyoderma, Dermatophytosis and mixed infestation were 31.37 per cent, 52.17 per cent, 26.47 per cent, 29.62 per cent, 39.13 per cent, 50.00 per cent and 46.67 per cent, respectively among the dogs between 1-3 years. Whereas, the prevalence of the same among 3-7 years and above 7 years were 52.94 and 15.68, 26.09 and 21.74, 44.12 and 29.41, 33.33 and 37.03, 21.74 and 39.13, 27.78 and 22.22 and 33.33 and 20.00 per cent, respectively. The prevalence of dermatoses in relation to gender of affected dogs revealed a highest rate in males (mange, pyoderma, dermatophytosis) in contrast to females (malasseziosis, atopic dermatitis, hypothyroidism and mixed infestation). All the cases included in the present study that were suspected for certain common dermatoses revealed similar manifestations such as pruritus, alopecia, erythema, papules, pustules, excoriations, scabs, crusts, scales and change in pigmentation. Few were also presented with rat tail appearance (hypothyroidism), urticaria, severe erythema of face, interdigits, otitis externa (atopy), greasy and offensive body odour (malasseziosis, pyoderma). Some of these cases were also presented with mixed ectoparasites like ticks, lice and fleas. Out of a total of 214 dermatoses dogs, 51 cases revealed positive for mites particularly Sarcoptes scabiei and Demodex canis. Tape impressions revealed blue colored footprint shaped yeast organisms that confirmed the diagnosis for malasseziosis. Ringworm infection suspected sample revealed positive for Trichophyton spp and Microsporum spp. Glass impression smears that were stained with gram’s stain were found positive for Staphylococcus spp thus, confirming pyoderma. Few of the cases suspected for fungal dermatosis showed a fluorescent apple green coloured areas where the lesions were present confirming Microsporum spp. Whereas, 27/214 dogs revealed significantly (P<0.05) elevated levels of serum cholesterol and triglycerides along with low thyroid profile, thus confirming the hypothyroid associated dermatosis. Following intradermal injection of various allergens, 38 dogs were found sensitive for cockroach male, followed by Parthenium, cockroach female, common dust, Aspergillus fumigatus, dust mites and Trichoderma, respectively which was manifested as an erythematous swelling similar to a bee sting with a sharp ridge at the peripheral margin within 15 minutes. The severity was recorded as +, ++, +++ and ++++ if the area was within 3 cm, 3-4 cm, 4-4.5 cm and more than 4.5cm. Scanning electron microscopy revealed Sarcoptes scabiei, Demodex canis, Malassezia pachydermatis and Trichophyton spp. Based on intra dermal allergic skin test, dogs of various breed, age and sex that were presented with erythema, pruritus and alopecia and diagnosed for canine atopic dermatitis were proportionately divided into 3 groups such as Group I, II and III with 15 in each and subjected for therapeutic trail. All the 15 dogs of Group I showed clinical improvement from day 7 with complete clinical recovery within 20 days of treatment. and the same among GII dogs started from day 10 with complete recovery among all the affected cases by day 30. Whereas, clinical improvement among group III dogs was gradual and slow from day 15 with complete clinical recovery by the end of therapeutic period (day 30) among only 11 cases. Among all the dogs of Group I, following therapy with topical tacrolimus spray twice daily, apart from clinical improvement from day 7, a significant improvement in hypersensitive reaction towards all the allergens was also noticed on day 10. Similarly among group II cases that received oral tacrolimus, twice daily, along with clinical improvement from day 7, a non significant improvement in hypersensitive reaction against intra dermal allergic skin test was noticed on day 10. However, a significantly (P<0.05) decreased hypersensitive zone was recorded on day 20. Whereas, improvement in group III cases was non-significant on day 20 but significantly different on day 30. However, there was a significant difference (P< 0.05) with respect to these parameters between group I, group II and III dogs, respectively.ThesisItem Open Access A COMPARATIVE STUDY OF NATURAL AND EXPERIMENTAL AFLATOXICOSIS WITH REFERENCE TO EPIZOOTIOLOGY, CLINICAL AND PATHOLOGIC FEATURES IN FINISHER SWINE(SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA, 1980) DHANVANTARI, D; NARAYANA RAO, P.L(MAJOR); KRISHNA SWAMY, S; SUBBA RAO, N