Browsing by Author "Vijay, K"
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ArticleItem Open Access Antioxidant Status in STZ-induced diabetic rats treated with Vanadium pentoxide nanoparticles(2019-12) Vijay, K; Suresh, R; Loganathasamy, K, et al.; TANUVASAntioxidant status determines the susceptibility of tissues to the oxidative stress associated with diabetes and its complications; hence in the present study antioxidant status was explored in streptozotocin-induced diabetic rats treated with vanadium in the form of vanadium pentoxide nanoparticles. Vanadium pentoxide and vanadium pentoxide nanoparticles at the dose rate of 5mg/kg were administered orally in STZ (50mg/Kg) induced diabetic rats for 30 days and glimepiride (reference drug) was administered orally at the dose rate of 800 μg/kg body weight. Vanadium pentoxide nanoparticles significantly reduced the blood glucose levels than the diabetic control and other treatment group of rats. On exploration of antioxidant status in liver, kidney and pancreas tissues, vanadium in the form of vanadium pentoxide nanoparticles outperformed the vanadium pentoxide by increasing the activities of the enzymes catalase, superoxide dismutase and glutathione peroxidase, and the concentration of reduced glutathione and by decreasing the lipid peroxide levels. The present study also showed that the restoration of antioxidant status by vanadium pentoxide nanoparticles is comparable with that of the reference drug. It can be concluded that vanadium pentoxide nanoparticles, due to its superior control over hyperglycemia and antioxidant properties, outperformed the vanadium pentoxide treatment in enhancing the antioxidant status in diabetic rats.ArticleItem Open Access Evaluation of Anti-tumour effects of Withaferin A Using Molecular Markers in a Rat Model of Mammary Carcinogenesis(2020-08) Pratheepa, K; Vijayarani, K; Balachandran, C; Sridhar, R; Vijay, K; TANUVASThe present study was designed to evaluate the anti-tumour potential of Withaferin A in DMBA (7,12-dimethylbenz[a]anthracene) induced rat mammary tumorigenesis. Seventy two female Sprague-Dawley rats were equally divided into control, DMBA, DMBA + tamoxifen (Standard drug) and DMBA +Withaferin A groups. DMBA (5 mg/rat/week/per os) at 4 weekly doses were used for tumour induction. Mammary tumours were collected on the 30th, 75th and 120th day after the initial dose of DMBA administration. The expression of p53, bcl-2, bax and PCNA was analysed by immunohistochemistry and RT-PCR. Oral administration of Withaferin A (16 mg/kg body weight/thrice a week/ per os) showed increased incidence of carcinomas by modulating markers of apoptosis (Bax, Bcl-2), cell survival (p53) and proliferation (PCNA) when compared to the standard drug tamoxifen (100 μg/kg body weight/day/per os).ArticleItem Open Access Non-Invasive Fecal Based PCR Assays for Detection of Mouse Parvo Virus and Minute Virus of Mice in Laboratory Mice(2021-11) Srinivasan, MR; Vijay, K; Karuppannan, AK, et al.,; TANUVASABSTRACT Background: Murine parvoviruses are a few of the most common pathogens of laboratory mice posing potential threats to mice colonies and experiments conducted in mice. We initiated this study to develop the fecal based PCR assays as an alternative to serology and to check its feasibility to detect the infections in mice caused by Mouse Parvo Virus (MPV) and Minute Virus of mice (MVM). Methods: Primers targeting the VP2 gene of MPV and MVM were selected and their sensitivity was analysed in tenfold serially diluted gene template in the presence of negative mouse fecal DNA. Selected thirty-seven mice at the age of 6 to 18 weeks randomly and collected blood samples for serology and fecal samples for PCR assays. Result: PCR assays of MPV and MVM detected as low as 0.4 fg of the target plasmid DNA. PCR assays in fecal samples of mice detected the presence of natural infections of MPV and MVM and their respective prevalence was 24% and 30%. Diagnostic sensitivity of MPV and MVM were 74% and 76% respectively. Our findings indicate that the fecal-PCR assay may be a useful, non-invasive and sensitive diagnostic tool in the ante-mortem detection of MPV and MVM in the health monitoring program.ArticleItem Open Access Rapid and Sensitive Faecal based PCR Assays for the Detection of Parvoviruses in Laboratory Rats(2020-08) Srinivasan, MR; Vijay, K; Ramesh, S; Karuppannan, AK; Krishnamohan Reddy, Y; TANUVASHealth status of laboratory rats indicates its suitability for the researches. They are prone to develop viral infections of subclinical nature caused by parvoviruses, which affects the research results adversely; Hence Implementation of health monitoring protocol is essential at the level of breeding colony itself. This study was intended to develop rapid and sensitive fecal-PCR assays to detect infections of multiple species of Parvoviruses affecting rats namely, Rat Minute Virus, Toolan’s Parvo Virus, Rat Parvo Virus and Kilham’s Rat Virus as an alternate approach to serology based health monitoring in a lab animal breeding unit. All the primers detected only in the presence of the respective templates. PCR assays of RMV and TPV consistently amplified as little as 40 fg and that of RPV and KRV consistently amplified as little as 4 fg of plasmid DNA. Specificity and sensitivity assays indicate that the PCR assays may be useful as diagnostic tools for rapid detection of natural acute viral infections. Further analysis of the primers in the positive laboratory animal colony is essential.ArticleItem Open Access RT-PCR Assay for Detection of Enterotropic Strains of Mouse Hepatitis Virus in Faecal Samples(2020) Srinivasan, MR; Vijay, K; Karuppannan, AK, et al.,; TANUVASABSTRACT Background: Mouse Hepatitis Virus (MHV) is one of the most important and common viral infections of laboratory mice, due to its highly contagious and subclinical nature, posing threat to the research outcomes. Periodic screening of laboratory mice for MHV is mandatory. Hence, this study was intended to develop sensitive faecal based RT-PCR assays to detect active infection of enterotropic MHV in laboratory mice. Methods: Primers targeting N-gene of MHV were selected and their sensitivity was analysed in tenfold serially diluted gene template in the presence of negative mouse faecal cDNA. Thirty-six weaned mice at the age of 6 to 18 weeks were randomly selected at different periods and the blood and faecal sample were collected for serology and RT-PCR assay respectively. RT-PCR assay in colon samples was carried out for comparison. Result: PCR assay of MHV detected as low as 4 fg of plasmid DNA. Seroprevalence of MHV is very high than the prevalence by RTPCR assay showing its retrospective nature and also seroprevalence includes both enterotropic and polytropic strain. RT-PCR results in faecal samples are analogous with that of the colon samples, showing the reliability of antemortem testing of mice for enterotropic strain of MHV.