Evaluation of Anti-tumour effects of Withaferin A Using Molecular Markers in a Rat Model of Mammary Carcinogenesis
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Date
2020-08
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Abstract
The present study was designed to evaluate the anti-tumour potential of
Withaferin A in DMBA (7,12-dimethylbenz[a]anthracene) induced rat
mammary tumorigenesis. Seventy two female Sprague-Dawley rats were
equally divided into control, DMBA, DMBA + tamoxifen (Standard drug)
and DMBA +Withaferin A groups. DMBA (5 mg/rat/week/per os) at 4
weekly doses were used for tumour induction. Mammary tumours were
collected on the 30th, 75th and 120th day after the initial dose of DMBA
administration. The expression of p53, bcl-2, bax and PCNA was analysed
by immunohistochemistry and RT-PCR. Oral administration of Withaferin
A (16 mg/kg body weight/thrice a week/ per os) showed increased
incidence of carcinomas by modulating markers of apoptosis (Bax, Bcl-2),
cell survival (p53) and proliferation (PCNA) when compared to the
standard drug tamoxifen (100 μg/kg body weight/day/per os).
Description
TNV_IJCMAS_2020_9(8)263-272
Keywords
Veterinary Science