RT-PCR Assay for Detection of Enterotropic Strains of Mouse Hepatitis Virus in Faecal Samples
Loading...
Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
ABSTRACT
Background: Mouse Hepatitis Virus (MHV) is one of the most important and common viral infections of laboratory mice, due to its
highly contagious and subclinical nature, posing threat to the research outcomes. Periodic screening of laboratory mice for MHV is
mandatory. Hence, this study was intended to develop sensitive faecal based RT-PCR assays to detect active infection of enterotropic
MHV in laboratory mice.
Methods: Primers targeting N-gene of MHV were selected and their sensitivity was analysed in tenfold serially diluted gene template
in the presence of negative mouse faecal cDNA. Thirty-six weaned mice at the age of 6 to 18 weeks were randomly selected at
different periods and the blood and faecal sample were collected for serology and RT-PCR assay respectively. RT-PCR assay in colon
samples was carried out for comparison.
Result: PCR assay of MHV detected as low as 4 fg of plasmid DNA. Seroprevalence of MHV is very high than the prevalence by RTPCR
assay showing its retrospective nature and also seroprevalence includes both enterotropic and polytropic strain. RT-PCR results
in faecal samples are analogous with that of the colon samples, showing the reliability of antemortem testing of mice for enterotropic
strain of MHV.
Description
TNV_IJAR_2020_90(7)1-5
Keywords
Veterinary Science