Browsing by Author "Tirumurugaan, K.G."
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ArticleItem Open Access Allelic Polymorphism of Exon 3 of Leptin Gene in Nilagiri Sheep Identified by Sequencing and Pcr-Rflp(2014-06) Cauveri, D.; Sivaselvam, S.N.; Karthikeyan, S.M.K.; Tirumurugaan, K.G.; Kumanan, K.; TANUVASThe discovery of hormone leptin had led to better understanding of the energy balance control. Leptin regulates feed intake, energy metabolism and body composition and plays a critical role in regulating body weight and growth in mammals. Characterization of Leptin gene (LEP) would not only help us understand the potential of this gene as marker but will also help us understand the genetic diversity of indigenous breeds and help in their conservation. In the present study the polymorphism in Exon 3 of LEP gene in Nilagiri sheep was done by sequencing and genotyping by PCR-RFLP. Five primers were designed to amplify the entire Exon 3 of LEP gene. Two polymorphisms 16973 G>A (SNP-L1) and 17476 C>T (SNP-L2) were detected in the Exon 3 in Nilagiri sheep. Animals of SNP-L1 were homozygous to the A allele. On genotyping the SNP-L2 by PCR-RFLP using restriction enzyme BsrDI, it was found that 77 per cent of the animals were of the CC genotype and 23 animals of the CT genotype. The allelic frequencies of C and T were 0.87 and 0.13 respectively. This shows that the LEP gene is polymorphic in Nilagiri sheep and PCR-RFLP is an appropriate tool to evaluate genetic variability.ArticleItem Open Access Allelic Polymorphism of Exon 3 of Leptin Gene in Nilagiri Sheep Identified by Sequencing and PCR-RFLP(2014) Cauveri, D.; Sivaselvam, S.N.; Karthickeyan, S.M.K.; Tirumurugaan, K.G.; Kumanan, K.; TANUVASThe discovery of hormone leptin had led to better understanding of the energy balance control. Leptin regulates feed intake, energy metabolism and body composition and plays a critical role in regulating body weight and growth in mammals. Characterization of Leptin gene (LEP) would not only help us understand the potential of this gene as marker but will also help us understand the genetic diversity of indigenous breeds and help in their conservation. In the present study the polymorphism in Exon 3 of LEP gene in Nilagiri sheep was done by sequencing and genotyping by PCR-RFLP. Five primers were designed to amplify the entire Exon 3 of LEP gene. Two polymorphisms 16973 G>A (SNP-L1) and 17476 C>T (SNP-L2) were detected in the Exon 3 in Nilagiri sheep. Animals of SNP-L1 were homozygous to the A allele. On genotyping the SNP-L2 by PCR-RFLP using restriction enzyme BsrDI, it was found that 77 per cent of the animals were of the CC genotype and 23 animals of the CT genotype. The allelic frequencies of C and T were 0.87 and 0.13 respectively. This shows that the LEP gene is polymorphic in Nilagiri sheep and PCR-RFLP is an appropriate tool to evaluate genetic variability.ThesisItem Open Access ANALYSIS OF BOVINE SPERM ASSOCIATED MEMBRANE PROTEINS BY TWO DIMENSIONAL GEL ELECTROPHORESIS(TANUVAS, Chennai, 2017) Muralidharan, T.; Palanisammi, A.; TANUVAS; Tirumurugaan, K.G.; Sivaselvam, S.N.This study was carried out in cattle sperm in the aspect of proteomics to find out the differences between the X- & Y- spermatozoa as far as the membrane proteins are concerned. It is a known fact that there are certain proteins present in the plasma membrane of the spermatozoa which are sex-specific and are called as sex associated or sperm associated membrane (SAM) proteins.ArticleItem Open Access Analysis of the Fusion Protein Cleavage Site of Newcastle Disease Virus Isolates from India Reveals Preliminary Evidence for the Existence of II, VI and VII Genotypes(Indian Journal of Virology, 2011-12) Tirumurugaan, K.G.; Vinupriya, M.K.; Vijayarani, K.; Kumanan, K.; TANUVASBook chapterItem Open Access CELL SIGNALING(TANUVAS, Chennai, 2009-09) Tirumurugaan, K.G.; TANUVASYou are in for a long story if you approach a cell biologist and get some explanation about signal transduction. The way by which basic cellular activities and co-ordination of cell actions is governed by a complex system of communication which together constitute Cell Signaling. The basis of normal development and tissue homeostatis, tissue repair and immunity depend on the cells to perceive and correctly respond to their micro environment.ThesisItem Open Access Characterisation of Bovine Insulin-Like Growth Factor-1 (IGF-1) Gene and its Association with Draught Power(TANUVAS, 2012) Gogoi, Ankita; TANUVAS; Karthickeyan, S.M.K.; Rahumathulla, P.S.; Tirumurugaan, K.G.ArticleItem Open Access CHARACTERIZATION OF GROWTH HORMOME (GH) GENE IN SHEEP BREEDS OF TAMIL NADU(Indian Society for Sheep and Goat Production and Utilization, 2017-10) Cauveri, D.; Sivaselvam, S.N.; Karthickeyan, S.M.K.; Tirumurugaan, K.G.; Kumanan, K.; TANUVASTo identify the polymorphisms in the native sheep breeds, characterization of growth hormone (GH) gene was carried out in eight breeds (Coimbatore, Kilakarsal, Madras Red, Mecheri, Nilagiri, Ramnad White, Tiruchy Black and Vembur) of Tamil Nadu. To amplify the whole GH gene, four primers that generated overlapping amplicons of a mean size of 762 bp were used. The PCR products from a minimum of 12 samples from each breed were sent for sequencing. Both the forward and reverse sequences obtained in the FASTA and *.ab1 format were compared with the reference sequence from NCBI (NC_019468 and GeneID: 443329) for identifying the SNPs. Three novel SNPs 480 G>A (SNP-G1), 871 G>A (SNP-G2) and 1259 A>C (SNP-G3) were identified in Intron 1, 2 and 3 of GH gene, respectively. In Intron 1, a 480 G>A (SNP-G1) transition was found in all the eight breeds. All the three genotypes viz. GG, GA and AA were found in Madras Red, Mecheri, Nilagiri and Tiruchy Black, whereas Coimbatore, Kilakarsal, Ramnad White and Vembur showed only GG and GA genotypes. SNP-G2, a G>A transition was specific to Nilagiri sheep. In all the other breeds only AA genotype was present (reference G). In Nilagiri the G allele was found as a heterozygote in 17% of the animals screened. No GG animals were identified. SNP-G3, an A>C transversion was identified in the Intron 3 region of Coimbatore, Kilakarsal, Madras Red and Ramnad White breeds. In Kilakarsal and Madras Red all three genotypes (AA, AC and CC) were found, whereas Coimbatore sheep had AA and AC genotypes and Ramnad White had only AC genotype. It was concluded that the exons of GH gene were highly conserved and did not show any polymorphism whereas the intons were polymorphic.ArticleItem Open Access Characterization of Nucleoprotein Gene Sequence of an Indian Isolate of Rabies Virus(Acta Virologica, 2004) Jayakumar, R.; Tirumurugaan, K.G.; Ganga, G.; Kumanan, K.; Nainar, A. Mahalinga; TANUVASThesisItem Open Access Closure of Large Skin Defects Using Tension Relieving/Tissue Expansion Techniques in Dogs(TANUVAS, 2013) Bhuvaneshwari, V.; TANUVAS; Shafiuzama, Mohamed; Jayaprakash, R.; Tirumurugaan, K.G.ArticleItem Open Access Co-culture: A Quick Approach For Isolation of Street Rabies Virus in Murine Neuroblastoma Cells(Veterinary World, 2015-05) Sasikalaveni, A.; Tirumurugaan, K.G.; Manoharan, S.; Raj, G. Dhinakar; Kumanan, K.; TANUVASBackground: Laboratory detection of rabies in most cases is based on detection of the antigen by fluorescent antibody test, however, in weak positive cases confirmative laboratory diagnosis depends on widely accepted mouse inoculation test. Cell lines like neuroblastoma have been used to isolate the virus with greater success not only to target for diagnosis, but also for molecular studies that determine the epidemiology of the circulating street rabies strains and in studies that look at the efficiency of the developed monoclonal antibodies to neutralize the different rabies strains. Due to the recent issues in obtaining ethical permission for mouse experimentation, and also the passages required in the cell lines to isolate the virus, we report herewith a co-culture protocol using the murine neuroblastoma (MNA) cells, which enable quicker isolation of street rabies virus with minimum passages. Objective: This study is not to have an alternative diagnostic assay, but an approach to produce sufficient amount of rabies virus in minimum passages by a co-culture approach in MNA cells. Materials and Methods: The MNA cells are co-cultured by topping the normal cells with infected cells every 48 h and the infectivity was followed up by performing direct fluorescent-antibody test. Results: The co-culture approach results in 100% infectivity and hence the use of live mouse for experimentation could be avoided. Conclusion: Co-culture method provides an alternative for the situations with limited sample volume and for the quicker isolation of virus which warrants the wild type strains without much modification.ArticleItem Open Access Comparative fluorescence in situ hybridisation (FISH) mapping of class I and II MHC genes on Bos indicus and Bubalus bubalis chromosomes(2015-08) Karthickeyan, S.M.K.; Rai, Amit Kumar; Tirumurugaan, K.G.; Kanakaraj, P.; TANUVASStudy on comparative gene mapping is one of the alternative approaches to easily locate the genes among the related species using the DNA sequences or probes derived from well-explored species. This would also enable to understand better the conservation of divergence of known sequences and thereby help determine the evolutionary lineage between them. Among the species in family Bovidae, lots of molecular and cytogenetic investigations have been carried out in cattle, unlike in other related species such as buffalo. MHC class I sequences had already been regionally assigned using isotopic in situ hybridization in cattle (Fries et al. 1986).ArticleItem Open Access Comparative in Vitro Toll-Like Receptor Ligand Induced Cytokine Profiles of Toda and Murrah Buffaloes - Identification of Tumour Necrosis Factor Alpha Promoter Polymorphism(Veterinary Immunology and Immunopathology, 2012) Vignesh, A.R.; Raj, G. Dhinakar; Dhanasekaran, S.; Tirumurugaan, K.G.; Raja, A.; TANUVASArticleItem Open Access Comparison of Tests for Detection of -Lactamase Staphylococcus aureus in Mastitis Cows(2014-08) Chandrasekaran, D.; Venkatesan, P.; Tirumurugaan, K.G.; Subapriya, S.; Nambi, A.P.; Thirunavukkarasu, P.S.; Vairamuthu, S.; TANUVASThis study aims to compare tests for the detection of -lactamase producing Staphylococcus aureus. A total of 116 Staphylococcus aureus isolates were collected from clinical mastitis cows and tested for -lactamase production by nitrocefin test and presence of blaZ gene by PCR. Out of 116 S.aureus, 12 isolates were positive for nitrocefin test and blaZ gene. When compared with PCR results, nitrocefin test showed 100 % sensitivity and 100 % specificity.ArticleItem Open Access Comparison of Tests for Detection of β-Lactamase Staphylococcus aureus in Mastitis Cows(Indian Veterinary Journal, 2015-05) Chandrasekaran, D.; Venkatesan, P.; Tirumurugaan, K.G.; Subapriya, S.; Nambi, A.P.; Thirunavukkarasu, P.S.; Vairamuthu, S.; TANUVASThis study aims to compare tests for the detection of β-lactamase producing Staphylococcus aureus. A total of 116 Staphylococcus aureus isolates were collected from clinical mastitis cows and tested for β-lactamase production by nitrocefin test and presence of blaZ gene by PCR. Out of 116 S. aureus, 12 isolates were positive for nitrocefin test and blaZ gene. When compared with PCR results, nitrocefin test showed 100% sensitivity and 100% specificity.ArticleItem Open Access Complete Genome Sequence of a Newcastle Disease Virus from a Coturnix coturnix japonica (Japanese Quail) Covey in India(2014) Bhuvaneswari, S.; Tirumurugaan, K.G.; Jones, Jagaraj Cyril; Kumanan, K.; TANUVASPresentationItem Open Access Correlation Between Interferon Gamma Expression Levels in Foot and Mouth Disease Virus Antigen Stimulated Buffalo and Cattle Mononuclear Cells and their Respective Promoter Sequences(TANUVAS, Chennai, 2007-08) Raj, G. Dhinakar; Subramanian, B. Mohanan; Senthuran, S.; Tirumurugaan, K.G.; Thiagarajan, D.ArticleItem Open Access Detection and Differentiation of Leptospires using Real-time Polymerase Chain Reaction(Indian Journal of Animal Sciences, 2010-04) Malathi, G.; Tirumurugaan, K.G.; Vijayarani, K.; Kumanan, K.; TANUVASOtherItem Open Access Detection of Lethal Mutation (A781G) in Growth Hormone Gene causing Early Embryonic Death in Vembur Sheep of Tamil Nadu(TANUVAS, 2014-12) Seevagan, M.; Jeichitra, V.; Rajendran, R.; Tirumurugaan, K.G.