Comparative fluorescence in situ hybridisation (FISH) mapping of class I and II MHC genes on Bos indicus and Bubalus bubalis chromosomes

Abstract
Study on comparative gene mapping is one of the alternative approaches to easily locate the genes among the related species using the DNA sequences or probes derived from well-explored species. This would also enable to understand better the conservation of divergence of known sequences and thereby help determine the evolutionary lineage between them. Among the species in family Bovidae, lots of molecular and cytogenetic investigations have been carried out in cattle, unlike in other related species such as buffalo. MHC class I sequences had already been regionally assigned using isotopic in situ hybridization in cattle (Fries et al. 1986).
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Keywords
Veterinary Science, Animal Genetics and Breeding
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