Browsing by Author "Thirumurugaan, KG"
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ThesisItem Open Access GENERATION OF NUCLEIC ACID APTAMERS WITH SELECTIVE AFFINITY TO X/Y BOVINE SPERMATOZOA(TANUVAS, 2016) Vinod, PS; Thirumurugaan, KG; TANUVAS; Dinakar Raj, G; Sivaselvam, SNControlling the sex-type of the sperm that is to participate in fertilization, can he an effective approach for sex-selection thereby an approach to improve farm animal economies. The commercial gender selection method uses flow cytometry and is patent protected and necessitates exploration of an alternative methodology. In this context aptamers are novel class of molecules equivalent to antibodies which help in identifying minor phenotype differences between an X- and Y-sperm and enable the capturing and separation of the two populations.ArticleItem Open Access Magnetoferritin: A Novel Magnetic Protein Cage Nanocarrier(Excellent Publishers, 2019-02) Bobade, Sumedha; Vijayarani, K; Thirumurugaan, KG; Thangavelu, A; Vairamuthu, S; Kalaiselvi, G; TANUVASNanotechnology is the rapidly expanding field and nanoparticles are omnipresent. Biological nanoparticles are assembled from molecules or atoms synthesized in a biological system. They include magnetosomes, lipoproteins, viruses, exosomes and ferritins.. A typical instance of a protein cage possessing this native biological function is ferritin. This engineered ferritin, which has the same architecture as natural H-ferritin, is termed magnetoferritin. The iron storage protein ferritin consists of a spherical polypeptide shell (apoferritin) and accommodates various metal ions. During the last two decades, the manipulation of protein cages for the encapsulation of single inorganic nanoparticles into their core to design novel hybrid bioinspired nanoparticles Such hybrid nanoparticles represent an opportunity for advanced nanotechnology applications in the nanodevices, disease diagnosis and therapy, drug delivery, vaccine development and bioassay.ThesisItem Open Access PHENOTYPIC AND MOLECULAR GENETIC STUDIES ON DRAUGHTABILITY IN UMBLACHERY CATTLE OF TAMIL NADU(TANUVAS, 2016) Kousalya Devi, M; Karthickeyan, SMK; Sivaselvam, SN; Thirumurugaan, KG; TANUVASDraught Animal Power (DAP) is one of the sustainable and renewable resources of energy. At present, even after mechanization of agriculture, 55 per cent of the total cultivable area is ploughed by draught animals. Yet, draughtability traits are the most neglected traits. Hence, this study was undertaken to assess the draughtability potential of Umblachery breed of cattle besides recording phenotypic performance and to associate the polymorphism in six candidate genes viz. Angiotensin I-converting enzyme (ACE), Adrenergic beta 2 receptor (ADRB2), Bradykinin beta 2 receptor (BDKRB2), Glutathione peroxidase 1 (GPX-1), Insulin-like growth factor-1 (IGF-1) and Vasculo endothelial factor alpha (VEGFA) with draughtability traits.ArticleItem Open Access Sequence characterization and screening for polymorphism in thecaspase recruitment domain 15 gene of goat (Capra hircus)(2016) Remi Treasa, Eugine; Ann Mary, B; Thirumurugaan, KG; Dhinakar Raj, G; Karthikeyan, SMK; Rajendran, R; TANUVAStThe NOD-like receptors (NLRs) are a group of intracellular germline encoded innate immune receptorsthat recognize conserved microbial structures in various invasive pathogens. Atleast 23 different NLRshave been identified with CARD15 being the major NLR playing an important role in the co-ordinationof adaptive immune response induced by the CARD15 agonist Muramyl dipeptide (MDP). CARD15 hasbeen reported to confer resistance or susceptibility to Crohn’s disease in humans and Johne’s disease inanimals. We amplified and sequenced the major region of the CARD15 gene (3042 bp) from three nativegoat breeds (Barbari, Tellicherry and Kanni), screened for polymorphisms and correlated the expressionlevels of MDP induced CARD15 mRNA and downstream cytokines with that of the polymorphisms. Thedifferent exons that contribute to the LRR domain of the CARD15 gene were screened from a 3 breed panelwith twenty un-related animals each. Four polymorphisms were located in the coding region of the gene(one each in exon 5 and 10 and two in exon 6) and one in the non-coding region of the gene (Intron 5–6).Two nonsynonymous substitutions R2441H (in Barbari and Tellicherry) and A2492G (in Tellicherry andKanni) were observed in exons 5 and 6 respectively.