Sequence characterization and screening for polymorphism in thecaspase recruitment domain 15 gene of goat (Capra hircus)
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Date
2016
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Abstract
tThe NOD-like receptors (NLRs) are a group of intracellular germline encoded innate immune receptorsthat recognize conserved microbial structures in various invasive pathogens. Atleast 23 different NLRshave been identified with CARD15 being the major NLR playing an important role in the co-ordinationof adaptive immune response induced by the CARD15 agonist Muramyl dipeptide (MDP). CARD15 hasbeen reported to confer resistance or susceptibility to Crohn’s disease in humans and Johne’s disease inanimals. We amplified and sequenced the major region of the CARD15 gene (3042 bp) from three nativegoat breeds (Barbari, Tellicherry and Kanni), screened for polymorphisms and correlated the expressionlevels of MDP induced CARD15 mRNA and downstream cytokines with that of the polymorphisms. Thedifferent exons that contribute to the LRR domain of the CARD15 gene were screened from a 3 breed panelwith twenty un-related animals each. Four polymorphisms were located in the coding region of the gene(one each in exon 5 and 10 and two in exon 6) and one in the non-coding region of the gene (Intron 5–6).Two nonsynonymous substitutions R2441H (in Barbari and Tellicherry) and A2492G (in Tellicherry andKanni) were observed in exons 5 and 6 respectively.
Description
TNV_SRR_2016_(138)37-45