Browsing by Author "THAKUR, KANIKA"
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ThesisItem Open Access Refinement of technology for micropropagation of carnation (Dianthus caryophyllus L.) cv. ‘Master’(UHF,NAUNI, 2015) THAKUR, KANIKA; KANWAR, KAMLESHABSTRACT The present investigation aims at “Refinement of technology for micropropagation of carnation (Dianthus caryophyllus L.) cv. ‘Master’”. Surface sterilized shoot buds cultured on control (MS + 3% sucrose + 0.8% agar-agar) supplemented with 2.0 mg/l BA which showed 77.77% establishment. Best medium for multiplication was MS medium supplemented with 2.0 mg/l BA producing 4.50 number of microshoots of shoot length of 2.44 cm, but as it resulted in hyperhydricity of shoots so medium supplemented with kinetin (2.0 mg/l) was used which produced 3.67 number of microshoots and 4.55 cm shoot length. Low cost medium containing 30 g/l table sugar, 50 g/l starch and aquaguard water produced 3.52 shoots with 3.01 cm shoot length. It was also observed that subculturing passage affected the average number of shoots per explant and also the shoot length which was highest in case of fifth subculturing resulting in 5.58 and 5.27 number of shoots per explant on control and low cost medium and shoot length of 4.66 cm and 3.53 cm , respectively. 62.49% rooting and 4.50 average roots per shoot with 1.61cm was found on 1/2 MS + 0.04% activated charcoal and 48.58% rooting with 3.50 average roots per shoot and 1.75 cm was observed on 1/2 MS + 50g/l starch + 0.04% activated charcoal + 15 g/l table sugar. The study has resulted in the reduction cost of the medium by substituting agar-agar and sucrose with starch and table sugar.ThesisItem Open Access STUDIES ON IN VITRO PROPAGATION OF VIRUS INDEXED STRAWBERRY (Fragaria x ananassa Duch.) ITS CONSERVATION AND GENETIC FIDELITY(UHF,NAUNI, 2019-06) THAKUR, KANIKA; GUPTA, RAKESHABSTRACT The present investigation on "Studies on in vitro propagation of virus indexed strawberry (Fragaria x ananassa Duch.) its conservation and genetic fidelity" was carried out at the Department of Biotechnology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.). The survey done for TRSV in strawberry was confirmed using DAS-ELISA which revealed that no virus was reported from all the three locations (Dhaulakuan, Shimla, Solan). Since the plant material was free from virus an efficient system for micropropagation of strawberry cvs., Selva, Sweet Charlie and Brighton using runner tips was developed. The runner tips was sterilized using 0.2 per cent carbendazim for 10 min + 0.1 per cent HgCl2 for 3 minutes, which were established on MS medium consisting of 0.5 mg/l BA and 0.2 mg/l IBA. The highest multiplication of the established runner tips was obtained when MS medium supplemented with 0.5mg/l BA + 0.20 mg/l IBA + 1.0 mg/l GA3 was used. The maximum rooting of the shoots was obtained on half strength MS medium supplemented with 0.5 mg/l IBA and their maximum hardening was obtained in potting mixture consisting of cocopeat. The various conservation techniques used revealed that the survival of the runner tips at 250C was the maximum but the low temperature condition of 40C was found suitable. The encapsulation of the runner tips was done using 0.5 mg/l BA with maximum regeneration after 4 weeks of storage. Mannitol when used at the rate of 2.5 per cent resulted in 74.19 per cent survival of the encapsulated runner tips after 1 month of storage. Out of the 23 ISSR primers used 17 were informative and revealed similarity between the mother and the micropropagated plants determining the genetic uniformity of the in vitro raised plants.