Refinement of technology for micropropagation of carnation (Dianthus caryophyllus L.) cv. ‘Master’
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Date
2015
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation aims at “Refinement of technology for micropropagation of
carnation (Dianthus caryophyllus L.) cv. ‘Master’”. Surface sterilized shoot buds cultured on control
(MS + 3% sucrose + 0.8% agar-agar) supplemented with 2.0 mg/l BA which showed 77.77%
establishment. Best medium for multiplication was MS medium supplemented with 2.0 mg/l BA
producing 4.50 number of microshoots of shoot length of 2.44 cm, but as it resulted in hyperhydricity
of shoots so medium supplemented with kinetin (2.0 mg/l) was used which produced 3.67 number of
microshoots and 4.55 cm shoot length. Low cost medium containing 30 g/l table sugar, 50 g/l starch
and aquaguard water produced 3.52 shoots with 3.01 cm shoot length. It was also observed that
subculturing passage affected the average number of shoots per explant and also the shoot length
which was highest in case of fifth subculturing resulting in 5.58 and 5.27 number of shoots per
explant on control and low cost medium and shoot length of 4.66 cm and 3.53 cm , respectively.
62.49% rooting and 4.50 average roots per shoot with 1.61cm was found on 1/2 MS + 0.04%
activated charcoal and 48.58% rooting with 3.50 average roots per shoot and 1.75 cm was observed
on 1/2 MS + 50g/l starch + 0.04% activated charcoal + 15 g/l table sugar. The study has resulted in
the reduction cost of the medium by substituting agar-agar and sucrose with starch and table sugar.
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