Browsing by Author "SHILPA, M."
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ThesisItem Open Access BIOCHEMICAL AND MOLECULAR CONFIRMATION OF RESISTANCE TO Mungbean Yellow Mosaic Virus (MYMV) IN MUNGBEAN GENOTYPES(UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU, 2020-09-30) SHILPA, M.; Manjunath, B.Among the biotic factors, Mungbean yellow mosaic virus (MYMV) is the major bottleneck for pulse producers. Fourteen mungbean genotypes procured from AVRDC, Taiwan were screened for MYMV resistance in field and laboratory conditions during Kharif 2019 and summer 2020 MYB-6, 7, 8, 9 and 12 were found to be resistant under field conditions by less PDI and AUDPC values. The confirmation of resistance was also done through estimation of biochemical enzymes like peroxidase, polyphenol oxidase, catalase, superoxide dismutase, phenols and sugars, and found comparatively higher levels in MYB-6, 7, 8, and 9 genotypes, whereas MYB-11(susceptible check) showed less activity of defense molecules. The molecular confirmation of MYMV resistance was tested using Resistant Gene Analog (RGA) in all 14 genotypes. The RGA was amplified at 90bp in resistant genotypes viz MYB-6, 7, 8 and 9. The host preference of whitefly Bemasia tabaci on mungbean genotypes was determined, no fecundity was observed on any of the mungbean lines in contrast to cotton plants. Since MYMV resistant lines MYB-6, 7, 8 and 9 showed resistance in both field and laboratory studies, hence these lines can be used as a YMV donor in breeding process and also can be release as a variety after conducting a multi location trails in MYMV hotspot conditions.ThesisItem Open Access STATISTICAL EVALUATION OF CLIMATE ON SILK COCOON PRODUCTION IN KARNATAKA(University of Agricultural Sciences, Bangalore, 2018-08-01) SHILPA, M.; Gowda, D.M.Sericulture is one of the important allied agricultural activities which provides subsidiary income to the farmers and plays an important role in poverty alleviation. Climate is one of the main determinants of silk cocoon production. Even slight variation in climatic factors will adversely affects silk worm growth and interm affects cocoon production. In the present study the monthly data of silk cocoon production and climatic parameters from 2012-13 to 2016-17 were obtained from the six selected locations viz., Kolar, Chikkaballapur, Ramanagara, Chamarajanagara, Mandya and Mysuru districts of Karnataka, in order to study the impact of climatic changes on silk cocoon production. To study the variation in climate change linear trend analysis was done and results indicated that temperature showed a declining trend where as rainfall and relative humidity had Increasing trends which were statistically significant in all six locations under study. Polynomial models were used to ascertain trends in silk cocoon production. The quadratic model was found to be the best fit with moderate R2 values for both multivoltine and bivoltine race cocoon production. Multiple linear regression analysis was carried out to assess impact of climate on silk cocoon production separately for multivoltine and bivoltine race. The results revealed that climatic parameters had a significant influence on silk cocoon production. Further, in the multiple regression model the variables rainfall, temperature and relative humidity had a negative impact on cocoon production, which indicates that any changes in values of these variables will have adverse effect on cocoon production.