Browsing by Author "Nandini, R"
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ThesisItem Open Access CORRELATION AND PATH COEFFICIENT ANALYSIS BETWEEN SEED YIELD AND ITS COMPONENT CHARACTERS IN M4 AND M5 GENERATIONS OF BAMBARA GROUNDNUT (Vigna subterranea (L.) Verdc.)(UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2015-07-10) UMESHA, NAIK; Nandini, RThesisItem Embargo GENETIC IMPROVEMENT IN GREEN GRAM (VIGNA RADIATA L. WILCZEK) FOR MUNG BEAN YELLOW MOSAIC VIRUS RESISTANCE USING RESISTANT GENE ANALOGUE (RGA) PRIMERS(University of Agricultural Sciences, Bangalore, 2022-10-08) MARIYAPPAN, S B; Nandini, RGreen gram belongs to the family Fabaceae which is a nutritionally rich crop with easily digestible protein. Yield losses in mungbean due to Mungbean Yellow Mosaic Virus (MYMV) is recorded upto 100% which are the major constraints in production. Hence, present investigation was undertaken to study the genetic variability for yield and yield related traits and to identify sources of resistance to MYMV using RGA primers inAdvanced Breeding Lines and germplasm during 2019-2021. The analysis of variance revealed statistically significant differences among the genotypes for eight quantitative characters and high heritability coupled with high genetic advance was recorded for seed yield per plant, pods per plant and 100 seed weight indicating the involvement of additive gene action. A strong positive correlation and significant direct effect was observed between seed yield, pod length, pods per plant, seeds per pod and plant height. On screening mungbean genotypes under natural field conditions for MYMV resistance, 25 of them were found to be resistant, 222 moderately resistant and 53 were moderately susceptible, while the 2 checks were susceptible based on DI, PDI and AUDPC. However, when the genotypes were screened using 4 RGA primers, VMR1 amplified in 24 genotypes at 445bp and CYR1 primer amplified in 13 genotypes at 1236bp. 24 genotypes were further screened under hot spot condition for MYMV resistance and found to be stable resistant source. Thus,13 genotypes which amplified in both primers taken as testers and 10 susceptible genotypes taken as lines. Combining ability analysis of crosses revealed significant variability for all the characters in parents and among crosses. Further, GGABL- 78 X GG-ABL-200 and GG-ABL-101 X GG-ABL-189 were identified as promising crosses based on per se performance, sca effects and standard heterosis and it can be utilized for isolation of transgressive segregants in further breeding programmeThesisItem Open Access GENETIC IMPROVEMENT OF BAMBARA GROUNDNUT (Vigna subterranea (L.) Verdc) THROUGH MUTATION BREEDING(UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2015-12-12) CHITTI, BHARATKUMAR; Nandini, RBambara groundnut (Vigna subterrnea L. (Verdc.) termed as a potential crop of future is said to have its origin in Africa. It is considered to be a completely balanced food rich in protein (25-28%) and quantitatively superior to other pulses due to essential amino acids methionine and lysine. The present investigation was undertaken to utilize mutation breeding to genetically improve the self pollinated underexploited protein rich crop. The objectives of the study include determination of LD50 value, genotypic sensitivity, effectiveness and efficiency of EMS and isolation of economically important lines in two varieties of Bambara groundnut SB-42 and S-165A.The seeds were treated with EMS concentrations ranging from 0.1 to 0.5 per cent. EMS concentration of 0.3 per cent was determined to be LD50 value for both the varieties. Comparing the mutagenic effect on various biological parameters S-165A was more sensitive than SB-42. At 0.3 per cent EMS concentration a higher number of chlorophyll mutations were recorded compared to other treatments. Morphological mutations in SB-42 were identified to be more than in the variety S-165A.In M1 generation a negative shift in plant height was observed per cent mean over control in all concentrations of EMS. In M2 generation, days to flowering, number of pods per plant, pod yield per plant, seed yield per plant and shelling percentage exhibited a higher per cent increase over control. Based on Chisquare test for yellow mosaic virus disease resistance, the resistance was governed by single recessive gene and mutant families F-91, F-94, F-35, F-92, F-105 in SB-42 and F- 9,F-86,F-1, F-7 and F-19 in S-165A families exhibited tolerance to yellow mosaic virus disease under natural conditions. Variability for characters like number of plants per plant, pod yield per plant and seed yield per plant in M3 generation was higher and mutant families F-62, F-82, F-105, F-35 in SB-42 and F-7, F-14, F-9, F-1 in S-165A recorded high yield, high protein and high methionine content.ThesisItem Open Access MORPHOLOGICAL AND MOLECULAR DIVERSITY IN ISOLATED MUTANT LINES AND DIFFERENT VARIETIES OF BAMBARA GROUNDNUT [Vigna subterranea (L.) Verdc.](UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2016-07-21) MANU, M. D; Nandini, RBambara groundnut (Vigna subterranea (L.) Verdc.) is one of the most neglected and underutilized pulse crop grown mostly in the semi-arid tropics of the African continent. The present investigation was undertaken to detect possible genetic diversity among 46 stabilized mutant lines of Bambara groundnut genotypes developed using gamma rays based on morphological diversity and molecular diversity using RAPD markers was studied during kharif 2015 in the Department of Genetics and Plant Breeding, UAS, GKVK and MRS, Hebbala, Bengaluru. Forty six genotypes of Bambara groundnut were subjected to genetic divergence using D2 statistics. All the 46 genotypes were grouped into six clusters. Cluster I was largest with twenty three genotypes. Intra-cluster and inter-cluster D2 values ranged from 0 to 26.56 and 30.24 to 103.16, respectively. The Maximum inter cluster distance was observed between cluster III and VI. The maximum contribution towards genetic divergence was due to number of leaves per plant (64.53 %). Genotypes from cluster II with VI, cluster I with VI and cluster I with V may serve as potential parents for hybridization programme. Genetic diversity was also evaluated using Random Amplified Polymorphic DNA (RAPD) markers. RAPDs revealed considerable levels of polymorphism among genotypes. The per cent polymorphism ranged from 0 % to 100 % with an average of 71.42 %. The construction of genetic relationships using cluster analysis grouped the 46 genotypes into two major clusters. RAPDs are useful for the genetic diversity studies in V. subterranea and can identify variation within genotypes.ThesisItem Open Access VARIABILITY STUDIES IN M3 GENERATION AND SCREENING FOR YELLOW MOSAIC VIRUS DISEASE RESISTANCE IN ISOLATED MUTANTS OF BAMBARA GROUNDNUT [Vigna subterranea (L.) Verdc.](UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2015-07-10) PRANESH; Nandini, RBambara groundnut (Vigna subterranea (L.) Verdc.) is an autogamous legume crop cultivated by the resource poor farmers in the African continent. The present investigations were undertaken to study the Genetic variability, to determine the correlation and path coefficient in M3 generation of Bambara groundnut variety ‘SB-42’ treated with Ethyl Methane Sulphonate and screening for yellow mosaic virus (YMV) disease resistance using heterologous probes in M6 mutant lines irradiated with gamma rays was also undertaken during kharif 2014 in Department of Genetics and Plant Breeding, UAS, GKVK, Bengaluru. In M3 generation a positive shift in mean was observed for all traits except days to flowering and plant height. Number of branches plant-1 showed high phenotypic coefficient of variance (24.53) and genotypic coefficient of variance (24.22). Higher heritability coupled with high genetic advance percent of mean was observed for the number of branches plant-1, plant spread, number of pods plant-1, pod yield plant-1, seed yield plant-1 reveals the higher genetic gain under selection. The pod yield plant-1 (0.94**), number of pods plant-1 and plant height had significant correlation with seed yield plant-1. Pod yield plant-1 (1.05), shelling percentage, number of pods plant-1, plant spread, 100 seed weight and plant dry weight had higher positive direct effect on seed yield plant-1. The designed SSR primers for the RGA 1F-CG/RGA 1R sequence were unable to amplify M6 mutant lines indicating that all the lines lack resistant source and to use more markers to identify the resistance to YMV disease in Bambara groundnut.