VARIABILITY STUDIES IN M3 GENERATION AND SCREENING FOR YELLOW MOSAIC VIRUS DISEASE RESISTANCE IN ISOLATED MUTANTS OF BAMBARA GROUNDNUT [Vigna subterranea (L.) Verdc.]
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Date
2015-07-10
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Bambara groundnut (Vigna subterranea (L.) Verdc.) is an autogamous legume
crop cultivated by the resource poor farmers in the African continent. The present
investigations were undertaken to study the Genetic variability, to determine the
correlation and path coefficient in M3 generation of Bambara groundnut variety ‘SB-42’
treated with Ethyl Methane Sulphonate and screening for yellow mosaic virus (YMV)
disease resistance using heterologous probes in M6 mutant lines irradiated with gamma
rays was also undertaken during kharif 2014 in Department of Genetics and Plant
Breeding, UAS, GKVK, Bengaluru.
In M3 generation a positive shift in mean was observed for all traits except days to
flowering and plant height. Number of branches plant-1 showed high phenotypic
coefficient of variance (24.53) and genotypic coefficient of variance (24.22). Higher
heritability coupled with high genetic advance percent of mean was observed for the
number of branches plant-1, plant spread, number of pods plant-1, pod yield plant-1, seed
yield plant-1 reveals the higher genetic gain under selection. The pod yield plant-1
(0.94**), number of pods plant-1 and plant height had significant correlation with seed
yield plant-1. Pod yield plant-1 (1.05), shelling percentage, number of pods plant-1, plant
spread, 100 seed weight and plant dry weight had higher positive direct effect on seed
yield plant-1. The designed SSR primers for the RGA 1F-CG/RGA 1R sequence were
unable to amplify M6 mutant lines indicating that all the lines lack resistant source and to
use more markers to identify the resistance to YMV disease in Bambara groundnut.
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