Browsing by Author "Nagarajan, T."
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ArticleItem Open Access Concentration of Progesterone Monoclonal Antibodies Using Different Non-Chromatographic Methods(Indian Journal of Animal Sciences, 2001-12) Nagarajan, T.; Raj, G. Dhinakar; Thiagarajan, V.; TANUVASHybridoma supernatants containing MAbs against progesterone (P) were concentrated using sephadex G-10, dralysorb, polyethylene glycol (PEG) 20000, Centriprep. PEG 6000 and saturated ammonium sulphate. Total protein concentration was estimated by Lowry’s method. Specific protein yield was estimated using capture ELISA by diluting the concentrates to pre-concentration level. The mean specific protein yiels were 85,65,70,71 and 59% with sephadex G-10, centriprep, dialysorb, saturated ammonium sulphate and PEG 6000 respectively. No recovery of specific protein was obtained with PEG 20,000. Based on the results, concentration of P. Mabs using sephadex G-10 appears to be an ideal, simple and rapid procedure for maximum recovery of P specific Mabs.ArticleItem Open Access Production and Characterisation Ofmonoclonal Antibodies to an Indian Isolate of Peste Des Petits Ruminants Virus(Small Ruminant Research, 2001) Raj, G. Dhinakar; Thiagarajan, V.; Chandrasekhar, M.; Nagarajan, T.; Nachimuthu, K.; TANUVASNine monoclonal antibodies (Mabs) were produced against an Indian isolate of peste des petis ruminants (PPR) virus. These Mabs were directed against the nucleo (N) protein and were of IgG1 isotype. The Mabs produced intranuclear or coarse granular cytoplasmic fluorescence in PPR virus infected Vero cells and did not exhibit any neutralising activity. The Mabs cross-reacted with five other local isolates of PPR virus in slot blot hybridisation, radio immunoprecipitation assay (RIPA) and fixed-cell enzyme linked immunosorbent assay (ELISA). Two of the nine Mabs cross-reacted mildly with the vaccine strain of rinderpest (RP) virus in slot blot hybridisation and fixed-cell ELISA but did not precipitate the N protein of RP virus in RIPA. The N protein specific Mabs will be highly useful in differential diagnosis of PPR from RP. © 2001 Elsevier Science B.V. All rights reserved.