Production and Characterisation Ofmonoclonal Antibodies to an Indian Isolate of Peste Des Petits Ruminants Virus
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Date
2001
Journal Title
Journal ISSN
Volume Title
Publisher
Small Ruminant Research
Abstract
Nine monoclonal antibodies (Mabs) were produced against an Indian isolate of peste des petis ruminants (PPR) virus. These Mabs were directed against the nucleo (N) protein and were of IgG1 isotype. The Mabs produced intranuclear or coarse granular cytoplasmic fluorescence in PPR virus infected Vero cells and did not exhibit any neutralising activity. The Mabs cross-reacted with five other local isolates of PPR virus in slot blot hybridisation, radio immunoprecipitation assay (RIPA) and fixed-cell enzyme linked immunosorbent assay (ELISA). Two of the nine Mabs cross-reacted mildly with the vaccine strain of rinderpest (RP) virus in slot blot hybridisation and fixed-cell ELISA but did not precipitate the N protein of RP virus in RIPA. The N protein specific Mabs will be highly useful in differential diagnosis of PPR from RP. © 2001 Elsevier Science B.V. All rights reserved.
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Keywords
Peste des petits ruminants, Monoclonal antibodies, Slot blot hybridisation, Radio immunoprecipitation assay, Fixed-cell enzyme linked immuosorbent assay