Concentration of Progesterone Monoclonal Antibodies Using Different Non-Chromatographic Methods
Loading...
Files
Date
2001-12
Journal Title
Journal ISSN
Volume Title
Publisher
Indian Journal of Animal Sciences
Abstract
Hybridoma supernatants containing MAbs against progesterone (P) were concentrated using sephadex G-10, dralysorb, polyethylene glycol (PEG) 20000, Centriprep. PEG 6000 and saturated ammonium sulphate. Total protein concentration was estimated by Lowry’s method. Specific protein yield was estimated using capture ELISA by diluting the concentrates to pre-concentration level. The mean specific protein yiels were 85,65,70,71 and 59% with sephadex G-10, centriprep, dialysorb, saturated ammonium sulphate and PEG 6000 respectively. No recovery of specific protein was obtained with PEG 20,000. Based on the results, concentration of P. Mabs using sephadex G-10 appears to be an ideal, simple and rapid procedure for maximum recovery of P specific Mabs.
Description
Keywords
Monoclonal Antibodies Progesterone Concentration Methods