Browsing by Author "Ganesan, P.I."
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OtherItem Open Access Anaplasmosis in postparturient phase in cattle and goats(TANUVAS, 1995-08) Ganesan, P.I.; Appaji Rao, V.N.; TANUVASDetailed Clinical and laboratory investigations on cases of post parturient anorexia and fever in 90 cattle and 11 goats that attended the Madras Veteirnary College Hospital from Octer '92 to Jan '93 realed infection with Anaplasma sp., in 41 fows, 34 baffaloes and 6 goats.ThesisItem Open Access Assessment of Cell Mediated and Mucosal Immune Responses in Chickens Vaccinated with Live Newcastle Disease Vaccines(TANUVAS, 2005) Varalakshmi, S.; TANUVAS; Kirubaharan, J. John; Chandran, N. Daniel Joy; Ganesan, P.I.OtherItem Open Access Biotechnological Perspectives on Tuberculosis : A Re-emerging Threat on Conservation of Asian Elephants(TANUVAS, Chennai, 2012-09) Subramanian, K.S.; Kumanan, K.; Chandran, N.D.J.; Ganesan, P.I.; Raja, A.; Ronald, B.S.M.; TANUVASArticleItem Open Access Comparative Efficacy of Single Intradermal Test and Gamma Interferon Assay in the Diagnosis of Bovine Tuberculosis(Indian Veterinary Journal, 2012-06) Ganesan, P.I.; TANUVASCell Mediated immune (CMI) responses are the principal immunological reactions in cattle to M. Bovis infection. The delayed type hypersensitivities, (DTH) as elicited by intradermal tuberculin reaction is not very specific and has several limitations in detecting tuberculosis in cattle. Serological techniques to detect circulating antibodies to M. Bovis are limited due to poor sensitivities and suboptimal specification (Costello et al., 1997). The present study compared the efficacy of tuberculin test and gamma interferon assay in detecting bovine tuberculosis.ThesisItem Open Access Comparative Study of Gamma Interferon Assay and Single Intradermal Test in the Diagnosis of Bovine Tuberculosis(TANUVAS, 2002) Latheef, Nishath; TANUVAS; Ganesan, P.I.; Nedunchelliyan, S; Ramadass, POtherItem Open Access Comparison of Single Intradermal Test and Gamma Interferon Assay for the Detection of Mycobacterium Avium Subsp. Paratuberculosis in Animals(TANUVAS, Chennai, 2007-08) Nagalakshmi, K. Shrine; Senthilkumar, T.M.A.; Ganesan, P.I.; Ramadass, P.ThesisItem Open Access Comparison of Single Intradermal Test and Polymerase Chain Reaction for the Diagnosis of Bovine Paratuberculosis in Organized Farms(TANUVAS, 2003) Kumar, O.R. Vinodh; TANUVAS; Ganesan, P.I.; Nedunchelliyan, S.; Raj, G. DhinakarThesisItem Open Access Comparison of Standard Tube Agglutination Test, Rose Bengal Test, Dot-Elisa and Elisa in the Diagnosis of Bovine Brucellosis(TANUVAS, 2004) Anuradha, P.; TANUVAS; Ganesan, P.I.; Kandavel, E.; Thangavelu, A.ArticleItem Open Access Comparison of Virus Isolation and Polymerase Chain Reaction for Diagnosis of Peste Des Petits Ruminants(Acta Virologica, 2001) Brindha, K.; Raj, G. Dhinakar; Ganesan, P.I.; Thiagarajan, V.; Nainar, A.M.; Nachimuthu, K.; TANUVASOculonasal swabs and tissue samples collected from peste des petits ruminants (PPR) suspected sheep and goats were tested for presence of the virus of peste des petits, ruminants (PPRV) or its RNA by reverse transcription-PCR (RT-PCR) and virus isolation (VI). Of 44 samples 31.8% and 40.9% were positive by VI and RT-PCR, respectively. The RT-PCR-positive samples were subjected to the nested PCR. Three of six samples positive by RT-PCR but negative by VI were negative by the nested PCR. The specificity and accuracy of the nested PCR were higher than those of the RT-PCR although the sensitivity of both tests were similar. Nucleotide sequencing of one nested PCR product revealed a 92% homology with the sequence available in the GenBank (Acc. No. Z37017).ArticleItem Open Access Cost of Feed in Production of Emu Eggs(Indian Veterinary Journal, 2012-06) Ganesan, P.I.; TANUVASThe emus (Dromains novaehollandiae) are reard for their eggs, meat and oil. The emus lay eggs during winter season and sometimes in summer (Minnar & Minnar, 1993). The cost of production of emu eggs depends upon raw material cost and the number of egg produced by individual hens. Though, the cost of production of emu hatching eggs, was reported by Nagabhushanrao et. al., (2005), the present study was carried out to know the cost of emu hatching eggs in Tamil Nadu.ArticleItem Open Access CROSS-SECTIONAL SEROSURVEY ON BOVINE BRUCELLOSIS IN CATTLE AND ITS EPIDEMIOLOGICAL SIGNIFICANCE IN INTEGRATED ORGANISED FARMS(Indian Veterinary Association Kerala, 2015-11) Preena, P.; Balakrishnan, S.; Ganesan, P.I.; Vijayabharathi, M.; Shereef, Shefeena; Kumar, V. Naveen; TANUVASBrucellosis in bovines is mainly caused by Brucella abortus inflicting late term abortion and in- fertility. Infected cattle act as a main source for transmission to other animals, animal handlers and veterinarians as an occupational hazard under organized farming system. Eventhough most Brucella species has host specificity, crossing of animal host boundary is likely to occur any time in swine, sheep and goat. The serosurveillance of animal health status has to be strictly regulated for effective control of brucellosis in integrated farms. The aim of this study was to determine the apparent prevalence of brucellosis in bovine from various organized farms in Tamil Nadu using ELISA. It was found that 21.35% of the cattle screened for brucellosis were positive for anti-Brucella abortus antibodies which may definitely have an impact on cross species transmission of brucellosis. Brucellosis being endemic in India, the effective control of brucellosis in all farm animals through vaccination programme and strict seroconversion studies is essential.ThesisItem Open Access Current Status of Bovine Brucella Abortus Infection in Selected Districts of Tamil Nadu(TANUVAS, 2005) Anandh, M. Jai; TANUVAS; Ganesan, P.I.; Jayakumar, R.; Thangavelu, A.OtherItem Open Access Detection and Identification of Mycobacteria From Trunk Wash of Captive Asian Elephants (Elephas Maximus)(TANUVAS, Chennai, 2012-09) Subramanian, K.S.; Ronald, B.S.M.; Kumanan, K.; Chandran, N.D.J.; Ganesan, P.I.; Raja, A.; Kavitharani, P.; Purushothaman, V.; TANUVASArticleItem Open Access Detection of Infectious Bovine Rhinotracheitis Antibodies in Cattle by Avidin-Biotin ELISA(Indian Veterinary Journal, 2013-03) Sathiyabama, K.; Ganesan, P.I.; TANUVASInfectious Bovine Rhinotracheitis (IBR), caused by Bovine Herpes Virus-1 (BHV-1) is the most economically important disease of cattle. In India, it was first reported by Mehrotra et al. (1976). The disease causes abortion, infertility, encephalitis, conjunctivitis, infectious pustular vulvo-vaginitis / balanoposthitis, respiratory problems and drop in milk production. Renukaradhya et al. (1996) detected 51.6% seropositivity to IBR from cattle in three Southern states of India. The present study describes the prevalence of IBR in cattle.ArticleItem Open Access Detection of Mycoplasma galliseptium infection in chickens from Tamil Nadu State of India(2019-01) Manimaran, K; Mishra, Adarsh; Hemalatha, S; Karthik, K; Ganesan, P.I.; TANUVASMycoplasma gallisepticum (MG) is one of the major respiratory tract pathogens affecting chickens. It causes Chronic Respiratory Disease (CRD) among chickens of various age groups. The present study describes the isolation, molecular detection and histopathological changes associated with CRD in chickens. A total of 790 samples viz., trachea, lungs and air sacs were collected from chickens showing the symptoms of CRD from different parts of Tamil Nadu state. All the samples were processed for isolation and molecular detection of MG. A total of 91 samples were found positive by isolation and 105 samples were found positive through MG specific PCR targeting 16S rRNA gene. The histopathological changes in tissue samples of trachea, sinuses, air sacs and lungs collected from naturally infected M. gallisepticum infection were suggestive of subacute to chronic nature of infection. Though isolation is considered to be a gold standard, still PCR is a rapid, sensitive and cheap method for early diagnosis of MG which can help poultry farmers to avoid severe economic loss.OtherItem Open Access Detection of Toxoplasma gondii in small ruminants in Chennai using PCR(GADVASU, 2016-02) Satbige, S. Ajay; Vijaya Bharathi, M.; Ganesan, P.I.; Sreekumar, C.; TANUVASA total of 193 sera samples, along with tissues (lung, heart, and brain] collected from 136 sheep and 57 goats from the Corporation slaughter house, Madras Veterinary College teaching hospital, and private mutton shops from Chennai were tested for Toxoplasma gondii. All the sera samples were tested using modified direct agglutination test (MAT). Of the 193 sera samples, 57 (29.5 per cent) had a minimum titre of 1:20. Of 57 samples, 30.14 per cent (41/136) ofsheep and 28.07 per cent (16/57) of goats being seropositive. Tissue samples from all 193 animals were subjected to B1 based PCR to detect T gondii DNA showed 3.67 per cent and 3.50 per cent to be positive in sheep and goats, respectively. in the present investigation B1 based PCR detected T.‘ gondii in low numbers, possibly due to limitation of the sample size. The presence of Tgondii in tissues of sheep and goats slaughtered for human consumption in Chennai indicates the role of these food animals as potential sources of infection to human.OtherItem Open Access Detection of Toxoplasma Gondii in Small Ruminants in Chennai Using PCR and modified Direct Agglutination Test(TANUVAS, 2016-02) Satbige, Ajay. S.; Bharathi, M. Vijaya; Ganesan, P.I.; Sreekumar, C.ThesisItem Open Access Development Of Recombinant Antigens For Diagnosis Of Avian Mycoplasmosis(Tamil Nadu Veterinary and Animal Sciences University, 2006) Venkatesh, G; TANUVAS; Ramadass, P; Raj, G. Dinakar; Prabhakar, T.G.; Ganesan, P.I.Avian Mycoplasmosis is widespread and is one of the most common respiratory diseases of poultry causing significant economic losses in poultry industry. The disease occurs when birds infected with Mycoplasma gallisepticum (MG) or Mycoplasma synoviae (MS) are stressed. An attempt has been made in this study to express the antigenic proteins of MG and MS in E.coli and use them in diagnostic assay. Primers were designed with built in restriction enzyme sites for amplification of p75 and p29 (TM-1) genes of MG PG31, PCR amplification of the genes was done, cloned and sequenced. The genes were cloned into expression vector pPROEx HTb and expressed in E. coli DH5α. The full length vlhA gene of local field isolate MS427 was amplified using published primers and the 5’ end corresponding to MSPB protein sequences were sequenced. Based on the sequence, primers were synthesized, amplified , a smaller part of MSPB (MSPB-I) and a larger part of MSPB (MSPB-II) gene was cloned in the expression vector pPROEx HTb and expressed in E. coli DH5α.The recombinant proteins were purified using Ni-NTA affinity chromatography.The four recombinant proteins obtained in this study were characterized and evaluated as diagnostic antigen. The recombinant P29 of MG and recombinant MSPB-II protein of MS showed a potential to be used as diagnostic antigens. A highly sensitive indirect ELISA, a rapid simple easy to perform latex agglutination test and flow-through enzyme immunoassay were developed to diagnose MG and MS separately using their respective recombinant proteins. The MG and MS indirect ELISA had perfect agreement with commercial ELISA kit used in this study. The latex agglutination tests and flow-through enzyme immunoassays had substantial agreement with commercial kit.