Detection of Toxoplasma gondii in small ruminants in Chennai using PCR
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Date
2016-02
Journal Title
Journal ISSN
Volume Title
Publisher
GADVASU
Abstract
A total of 193 sera samples, along with tissues (lung, heart, and brain] collected from 136 sheep and 57
goats from the Corporation slaughter house, Madras Veterinary College teaching hospital, and private
mutton shops from Chennai were tested for Toxoplasma gondii. All the sera samples were tested using
modified direct agglutination test (MAT). Of the 193 sera samples, 57 (29.5 per cent) had a minimum
titre of 1:20. Of 57 samples, 30.14 per cent (41/136) ofsheep and 28.07 per cent (16/57) of goats being
seropositive. Tissue samples from all 193 animals were subjected to B1 based PCR to detect T gondii DNA
showed 3.67 per cent and 3.50 per cent to be positive in sheep and goats, respectively. in the present
investigation B1 based PCR detected T.‘ gondii in low numbers, possibly due to limitation of the sample
size. The presence of Tgondii in tissues of sheep and goats slaughtered for human consumption in Chennai
indicates the role of these food animals as potential sources of infection to human.
Description
TNV_Com_Abs_Feb2016_17-19_Pg53
Keywords
Veterinary Medicine