Browsing by Author "Agarwal, S.K."
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ArticleItem Open Access Effect Of Non-Surgical Removal of Conceptus on Estrous Cycle Length and Interferon TAU (IFNT) Gene Expression Profile in Water Buffalo (Bubalus bubalis)(TANUVAS, Chennai, 2021-05) Sarath, T.; Singh, S.K.; Agarwal, S.K.; Arunmozhi, N.; Reddy, G.B. Manjunatha; Saxena, A.; TANUVASThe physiology of maternal recognition of pregnancy (MRP) in cattle is well explored and a better understanding of phenomenon of MRP is needed in order to improve reproductive efficiency in buffaloes. The present work was conducted to study the effect of non-surgical removal of conceptus and expression profile of interferon tau (IFNT) in the conceptus after post insemination in buffaloes. In the experiment I, 15 female buffaloes in estrus were inseminated using frozen semen of Murrah buffalo and the uterine flushing by non-surgical method was carried out on days 12, 14, 16, 18 and 20 of post insemination for collection of conceptus. Under experiment II, conceptus tissue collected on different days in the experiment I and conceptus tissue collected from slaughter house pregnant reproductive tracts were used for RNA isolation to study real time expression pattern of IFNT on day 12, 14, 16, 18, 20, 30, 40 and 50 of pregnancy by using specific primers (F5’-GCCCTGGTGCTGGTCAGCTA -3’ and R5’-CATCTTAGTCAGCGAGAGTC -3’).ThesisItem Open Access Modulation of progesterone production by luteal cells and histomorphological changes of corpus luteum during pregnancy in baffalo(IVRI, Izatnagar, 2010) Baithalu, R. Kumari.; Agarwal, S.K.ArticleItem Open Access Monitoring of Embryonic and Fetal Development Using Real Time ‘B’ Mode Ultrasound in Buffalo (Bubalus bubalis)(TANUVAS, Chennai, 2021-05) Sarath, T.; Singh, S.K.; Arunmozhi, N.; Saxena, A.; Shankar, Uma; Agarwal, S.K.; TANUVASThe present study was conducted in buffaloes which were maintained at experimental animal sheds of Animal Reproduction Division, IVRI, Izatnagar. All the experimental buffaloes (n=6) were observed for onset of estrus using a teaser bull and visual signs of estrus followed by rectal examination twice daily. Animals detected in estrus were inseminated using frozen semen of Murrah buffalo. The inseminated buffaloes were allowed for real time B-mode ultrasonographic examination at 10 days interval starting from day 20 to 120 of pregnancy. Monitoring of embryonic development was carried out by viewing embryonic vesicle diameter (EVD), Crown Rump length (CRL), embryonic heartbeat, skull and placentome through ultrasonography. The mean diameter of EV was 0.52±0.12, 1.56±0.16, 2.94±0.07, 3.80±0.14 and 6.37±0.15 cm on day 20, 30, 40, 50 and 60 of pregnancy, respectively. EVD increased with advancement of pregnancy and reached around 6.37±0.15 cm on day 60 of pregnancy.ArticleItem Open Access Morphological and Functional Characterization of Buffalo Endometrial Epithelial Cells Cultured In vitro(2015-09) Rajkumar, R.; Agarwal, S.K.; Singh, S.K.; Kumar, Anuj; TANUVASThe present work was designed to establish buffalo endometrial epithelial cell culture and to observe their morphological and functional properties. Endometrial epithelial cells were isolated using 0.3% trypsin and cultured in vitro. The epithelial cells appeared in clumps and attached to matrigel coated surface as small colonies within 48 hrs and were spherical or polygonal in shape. Epithelial cells reached confluence by 7 to 8 days and exhibited cuboidal morphology. The basal PGF2α production by the endometrial cells was significantly higher (2.8 fold) than PGE2. Addition of oxytocin significantly increased PGF2α (5 fold) production as compared to basal level.