Morphological and Functional Characterization of Buffalo Endometrial Epithelial Cells Cultured In vitro
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Date
2015-09
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Abstract
The present work was designed to establish buffalo endometrial epithelial cell culture and to observe their morphological and functional properties. Endometrial epithelial cells were isolated using 0.3% trypsin and cultured in vitro. The epithelial cells appeared in clumps and attached to matrigel coated surface as small colonies within 48 hrs and were spherical or polygonal in shape. Epithelial cells reached confluence by 7 to 8 days and exhibited cuboidal morphology. The basal PGF2α production by the endometrial cells was significantly higher (2.8 fold) than PGE2. Addition of oxytocin significantly increased PGF2α (5 fold) production as compared to basal level.
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Veterinary Science, Veterinary Gynaecology and Obstetrics