Effect Of Non-Surgical Removal of Conceptus on Estrous Cycle Length and Interferon TAU (IFNT) Gene Expression Profile in Water Buffalo (Bubalus bubalis)
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Date
2021-05
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS, Chennai
Abstract
The physiology of maternal recognition of pregnancy (MRP) in cattle is well
explored and a better understanding of phenomenon of MRP is needed in order to improve
reproductive efficiency in buffaloes. The present work was conducted to study the effect of
non-surgical removal of conceptus and expression profile of interferon tau (IFNT) in the
conceptus after post insemination in buffaloes. In the experiment I, 15 female buffaloes in
estrus were inseminated using frozen semen of Murrah buffalo and the uterine flushing by
non-surgical method was carried out on days 12, 14, 16, 18 and 20 of post insemination
for collection of conceptus. Under experiment II, conceptus tissue collected on different
days in the experiment I and conceptus tissue collected from slaughter house pregnant
reproductive tracts were used for RNA isolation to study real time expression pattern of
IFNT on day 12, 14, 16, 18, 20, 30, 40 and 50 of pregnancy by using specific primers
(F5’-GCCCTGGTGCTGGTCAGCTA -3’ and R5’-CATCTTAGTCAGCGAGAGTC -3’).
Description
TNV_IJVASR_50(3)_May-June.2021_51-60
Keywords
Veterinary Science, Animal Reproduction