STUDIES ON YELLOW MOSAIC VIRUS DISEASE OF SOYBEAN [Glycine max (L.) Merrill.]
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Date
2016-08-08
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Soybean yellow mosaic virus (SYMV) disease caused by a begomovirus is a
major constraint in soybean cultivation in Karnataka. SYMV was successfully
transmitted through whitefly (Bemisia tabaci). Hundred per cent virus transmission
was obtained by using ten adult whiteflies per plant with 12 hr of acquisition access
period (AAP), inoculation access period (IAP) and incubation period. One week old
soybean plants were susceptible for SYMV infection. SYMV was successfully
transmitted to soybean, horse gram, french bean, pole bean, lima bean, green gram
and rice bean. The virus was detected by polymerase chain reaction (PCR) technique
using viral coat protein (CP) and movement protein (MP) gene specific primers. PCR
product of 1000 and 900 bp sized corresponding to CP and MP genes of the virus
were amplified from total DNA obtained from SYMV infected leaf tissue. The
nucleotide sequences of the CP and MP genes showed maximum nucleotide identity
of 98 per cent and 99 per cent respectively with horse gram yellow mosaic virus
(HgYMV) isolates infecting french bean and lima bean. Phylogenetic analysis of the
sequences of CP and MP genes of SYMV revealed that virus isolate (GKVK)
clustered with HgYMV infecting french bean and lima bean isolates of Bengaluru,
which indicate that the virus causing SYMV disease is a strain of HgYMV. Out of
forty three genotypes of soybean screened for SYMV under glass house condition,
eleven genotypes viz., KDS-378, RKS-118, Bragg, DS-2706, DSb-25, Dsb-23,
MAUS-2, DSb-21, DS-2708, DS-2006 and DS-2707 were found moderately
resistance to SYMV.
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