CHARACTERIZATION OF VIRUSES ASSOCIATED WITH LEAF CURL AND STEM NECROSIS DISEASES OF BLACKGRAM IN ANDHRA PRADESH

Simple item page
dc.contributor.advisorSARADA JAYALAKSHMI DEVI, R
dc.contributor.authorPRASAD, KURAKU
dc.date.accessioned2020-02-24T05:07:56Z
dc.date.available2020-02-24T05:07:56Z
dc.date.issued2019
dc.descriptionD5871en_US
dc.description.abstractSurvey was carried out for disease incidence of leaf curl and stem necrosis during rabi 2018-19 in three blackgram growing districts of A.P. The mean disease incidence ranged from 4.10-6.26 %. The disease was successfully transmitted onto cowpea and back to blackgram from cowpea by mechanical inoculations. It produce typical local lesions and veinal necrosis on cowpea and leaf curling, necrosis of petiole, buds, leaves and other parts on blackgram. Total RNA was isolated and amplified by RT-PCR with GBNV/ TSV primers. An amplicon of 800bp was obtained with GBNV but not with TSV primers. About 800bp product was purified, sequenced and determined its length as 831 nucleotides and codes for 276 amino acids. The sequence homology studies with other GBNV isolates from data base shared 93-100 % and 94.5% to 100% sequence identity with GBNV at the nucleotide and amino acid levels, respectively. Phylogenetic tree was generated by comparing with 20 other reported Tospoviruses at nucleotide level by neighbour joining method and present isolate formed unique cluster with GBNV isolates. Among 21 genotypes screened by artificial inoculations, 5 genotypes (NDUK-15-222, TU-40, OBG-32, GKB-3, KPU-12-213) were found disease free, 2 genotypes (TBG-130, VBG-12-111-1) were found resistant, 4 genotypes (GBG-1, TBG-129, LBG-20, IPU-2-43) were found moderate resistance, 6 genotypes (LBG-645, LBG-623, TBG-104, SRI, LBG-787, TUTIMINUMU) were found susceptible, 4 genotypes (LBG-752, LBG-685, PU-31, BUTTAMINUMU) were found highly susceptible.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810143464
dc.keywordsLEAF CURL, STEM NECROSIS DISEASES, BLACKGRAM, ANDHRA PRADESHen_US
dc.language.isoen_USen_US
dc.pages112en_US
dc.publisherACHARYA N G RANGA AGRICULTURAL UNIVERSITY, GUNTURen_US
dc.research.problemCHARACTERIZATION OF VIRUSES ASSOCIATED WITH LEAF CURL AND STEM NECROSIS DISEASES OF BLACKGRAM IN ANDHRA PRADESHen_US
dc.subPlant Pathologyen_US
dc.subjectnullen_US
dc.themeCHARACTERIZATION OF VIRUSES ASSOCIATED WITH LEAF CURL AND STEM NECROSIS DISEASES OF BLACKGRAM IN ANDHRA PRADESHen_US
dc.these.typeM.Scen_US
dc.titleCHARACTERIZATION OF VIRUSES ASSOCIATED WITH LEAF CURL AND STEM NECROSIS DISEASES OF BLACKGRAM IN ANDHRA PRADESHen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
FINAL DOCUMENT.pdf
Size:
3.45 MB
Format:
Adobe Portable Document Format
Description:
CHARACTERIZATION OF VIRUSES ASSOCIATED WITH LEAF CURL AND STEM NECROSIS DISEASES OF BLACKGRAM IN ANDHRA PRADESH
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses