DEVELOPMENT OF NEW CLONES OF SALIX (WILLOW) THROUGH CONTROL BREEDING
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Date
2015
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Abstract
ABSTRACT
The present investigation entitled “Development of new clones of Salix (willow) through control
breeding” was carried out in the Department of Tree Improvement and Genetic Resources, Dr Y. S. Parmar
University of Horticulture & Forestry Nauni, Solan (HP) during 2013-2015. There is a collection of Salix
clones/ strains/ species procured from different countries covering five continents namely Europe, North
America, South America, Asia and Africa and maintained in the germplasm block of Naganji nursery from
which eight clones were included in the study and the plant material of 4 females (PN 227, J 799, S. babylonica
and S. tetrasperma ) and 4 male (NZ 1140, Austree, J 795 and S. tetrasperma ) clones were crossed using Line x
Tester (4 x 4) mating design. Control crossing was done and seedlings were raised in the nursery in RBD at
stage-1 and were evaluated for morphological characters. In stage-2 clonal cuttings of selected individuals and
parents were raised in RBD and were evaluated for morphological, physiological and wood characters. Line x
tester analysis for combining ability revealed that that line PN 227 and tester Austree were found to be good
general combiners. On the basis of heterosis over better parent, mean performance and significant desirable
SCA effects for morphological, physiological and wood characters the combinations PN 227 x Austree, PN 227
x NZ 1140 and PN 227 x J 795 were found to be the most promising families.The estimates of GCA variance
( 2 GCA) were more than the SCA variance ( 2 SCA) for all the characters studied. The gene action study
revealed that additive variance was observed more than the dominance variance for all the parameters studied.
The proportional contribution of lines was higher than individual contribution of testers or line × tester
interaction except for fresh root weight, dry shoot weight, dry root weight, total dry weight, wood density and
fibre length. The 10 SSR markers data disclosed that 12 hybrids were highly similar to their female parents and
whereas 4 hybrids were more similar to the male parent. On the basis of population structure analysis it is
concluded that this hybrid population structure is diverse and in future such information will be useful for
conducting association mapping for the traits of economic importance.
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Keywords
hybrids, crossing over, genetics, biological phenomena, planting, cloning, heterosis, wood, biological development, vegetative propagation, Salix (Willow), Controlled Breeding