STUDIES ON THE INCIDENCE OF SHIGELLA IN LIVESTOCK PRODUCTS AND ENVIRONMENTAL SAMPLES

dc.contributor.advisorKRISHNAIAH, N(MAJOR)
dc.contributor.advisorMADHAVA RAO, T
dc.contributor.advisorANJANEYULU, Y
dc.contributor.authorDURGA RAMA DEVI, N
dc.date.accessioned2018-11-12T09:39:11Z
dc.date.available2018-11-12T09:39:11Z
dc.date.issued2010-07
dc.descriptionTHESESen_US
dc.description.abstractABSTRACT: Out of 60 samples each of stools, drinking water, farm water, beef, chicken, egg and fish, Shigella spp. were found in 12 ,4, 15, 4, 3, 4, 3 and 5 respectively. Out of 12 positive stool samples for Shigella, 8 were S. flexneri, 3 were S. sonnei and 1 was S. dysenteriae. Out of 7 and 15 positive samples of drinking and farm water, S. flexneri was found in 4 and 10, S. sonnei 2 and 3 respectively and S. dysenteriae was one in each. Out of 4 positive samples each of beef and milk. S. flexneri was found in 3 and 2 S. sonnei one each and S. dysenteriae was 1 in milk and not found in beef. Out of 3, 3 and 5 positive samples of chicken, eggs and fish, S. flexneri was found in 2, 2 and 4 respectively. S. sonnei was found in one each and S. dysenteriae was not found. Out of 480 total different samples 53 were positive for Shigella spp., out of which 35 were S. flexeneri, 13 were S. sonnei and 4 were S. dysenteriae. For isolation and identification of Shigella spp., BHI broth enrichment was good for S. flexneri and SB broth was good for S. sonnei and S. dysenteriae. The isolation with or without enrichment and plating has shown that XLD and HEA media are superior over DCA, MAC and SS agar for S. flexneri and S. sonnei, where as for S. dysenteriae XLD, DCA, HEA and MAC has given moderate growth, with no growth on SS medium. In general, all the three enrichment broths have given better growth compared to direct plating. S. flexneri was highly sensitive to Kanamycin (94.28%) and Nalidixic acid (84.7%) and highly resistant to Gentamycin (97%) and Ampicillin (91.4%), where as S.sonnei was highly sensitive to Kanamycin (92.3%), Ampicillin (84.6%), Chloramphenical (69.23%) and highly resistant to Gentamycin (92.3%), Nalidixic acid (76.9%) and Cotrimaxozole (69.23%). S.dysenteriae was sensitive only to Kanamycin (75%) and Ciprofloxacin (25%) and resistant to Ampicillin, Gentamycin, Tetracycline , Nalidixic acid , Erythromycin, Chloramphenical and Cotrimoxazole (75%).en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810083259
dc.keywordsLIVESTOCK PRODUCTS;SHIGELLA;ENVIRONMENTAL SAMPLESen_US
dc.language.isoenen_US
dc.pages120en_US
dc.publisherSRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIAen_US
dc.subVeterinary Public Healthen_US
dc.subjectnullen_US
dc.themeSTUDIES ON THE INCIDENCE OF SHIGELLA IN LIVESTOCK PRODUCTS AND ENVIRONMENTAL SAMPLESen_US
dc.these.typeM.V.Sc.en_US
dc.titleSTUDIES ON THE INCIDENCE OF SHIGELLA IN LIVESTOCK PRODUCTS AND ENVIRONMENTAL SAMPLESen_US
dc.typeThesisen_US
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