GENE CLONING AND SEQUENCE ANALYSIS OF CHICKEN INFECTIOUS ANAEMIA (CIA) VIRUS

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dc.contributor.advisorDr. M. K. Jhala
dc.contributor.authorCHANNABASAYYA HIREMATH
dc.date.accessioned2017-07-14T05:47:06Z
dc.date.available2017-07-14T05:47:06Z
dc.date.issued2008
dc.description.abstractChicken infectious anemia virus (CIAV) is an economically important and contagious avian pathogen with a worldwide distribution, causing chicken infectious anemia (CIA) in chickens. CIAV is a non-enveloped, icosahedral virus having a single stranded circular DNA genome and belongs to genus Gyrovirus, within the family Circoviridae. In the present study, the suspected CIA incidences were confirmed by PCR applied to different affected tissues collected from clinically affected birds from poultry flocks in and around Anand district of Gujarat. To study the extent of genetic variability of the field CIAV, the nucleotide sequences of VP1, VP2 and VP3 genes of CIAV were determined directly from the cloned PCR products, without isolating the virus in cell culture to obviate the potential genetic nucleotide changes that can possibly occur during cell culture passage. The gene sequences and the deduced aminoacid sequences of VP1, VP2 and VP3 of CIAV were compared with the other Indian and foreign sequences available in GeneBank and phylogenetic analysis based on nucleotide and deduced aminoacid sequences of VP1 was carried out to access the genetic distance of CIAV circulating in the field.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810025199
dc.keywords"GENE CLONING AND SEQUENCE ANALYSIS OF CHICKEN INFECTIOUS, CHICKEN INFECTIOUS, ANAEMIA (CIA) VIRUSen_US
dc.language.isoenen_US
dc.publisherAnand Agricultural University, Ananden_US
dc.research.problemGENE CLONING AND SEQUENCE ANALYSIS OF CHICKEN INFECTIOUS ANAEMIA (CIA) VIRUSen_US
dc.subVeterinary Microbiologyen_US
dc.subjectVeterinary Microbiologyen_US
dc.subjectAnalysisen_US
dc.themeGENE CLONING AND SEQUENCE ANALYSIS OF CHICKEN INFECTIOUen_US
dc.these.typeM.V.Sc.en_US
dc.titleGENE CLONING AND SEQUENCE ANALYSIS OF CHICKEN INFECTIOUS ANAEMIA (CIA) VIRUSen_US
dc.typeThesisen_US
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