GENE CLONING AND SEQUENCE ANALYSIS OF CHICKEN INFECTIOUS ANAEMIA (CIA) VIRUS
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Date
2008
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Anand Agricultural University, Anand
Abstract
Chicken infectious anemia virus (CIAV) is an economically important and
contagious avian pathogen with a worldwide distribution, causing chicken infectious
anemia (CIA) in chickens. CIAV is a non-enveloped, icosahedral virus having a single
stranded circular DNA genome and belongs to genus Gyrovirus, within the family
Circoviridae. In the present study, the suspected CIA incidences were confirmed by
PCR applied to different affected tissues collected from clinically affected birds from
poultry flocks in and around Anand district of Gujarat. To study the extent of genetic
variability of the field CIAV, the nucleotide sequences of VP1, VP2 and VP3 genes of
CIAV were determined directly from the cloned PCR products, without isolating the
virus in cell culture to obviate the potential genetic nucleotide changes that can possibly
occur during cell culture passage. The gene sequences and the deduced aminoacid
sequences of VP1, VP2 and VP3 of CIAV were compared with the other Indian and
foreign sequences available in GeneBank and phylogenetic analysis based on
nucleotide and deduced aminoacid sequences of VP1 was carried out to access the
genetic distance of CIAV circulating in the field.
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Keywords
Veterinary Microbiology, Analysis