IDENTIFICATION OF FERTILITY RESTORERS AND MOLECULAR CHARACTERIZATION OF RESTORER LINES OF PIGEONPEA (Cajanus cajan L. Millsp.)
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Date
2014-06-10
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University of Agricultural Sciences GKVK, Bangalore
Abstract
An investigation on stability of male sterility, identification of
fertility restorers, heterosis, combining ability, stability of hybrids and
molecular characterization of restorer lines of pigeonpea was carried out
at experimental plots of the AICRP on Pigeonpea, UAS, GKVK, Bangalore.
Study on stability of male sterility of nine CMS lines over three seasons
at Bangalore and Hyderabad locations revealed that the CMS lines BRG
3A, Hy 3cA, TTB 7A and ICPA 2039 found to be stable at all the three
dates of pollen sterility assay. In fertility restoration analysis with three
male sterile lines, 14 out of 16 tested male lines restored more than 80%
pollen fertility of ICPA 2047 while, 12 and eight male lines restored pollen
fertility of ICPA 2092 and ICPA 2089 respectively. In combining ability
and heterosis studies over four environments, the hybrids, gca and sca;
and their various interactions with environment were significant
suggesting that the hybrids did not have the same relative performances
among environments. The CMS line ICPA 2047 and testers ICPL 20128,
ICPL 20107 and ICPL 20177 were found to be desirable combiners. For
seed yield per plant, the hybrids ICPA 2092 × ICPL 11376, ICPA 2047 ×
ICPL 20128 and ICPA 2089 × ICPL 20166 were the best specific
combinations. None of the hybrids were found stable across
environments for most of traits under consideration. The hybrids ICPA
2047 × ICPL 20128, ICPA 2092 × ICPL11376 and ICPA 2047 × ICPL
20177 were found most heterotic over standard check (ICPH 2671) for
seed yield. Out of eight SSR markers tested, two markers namely CcM
2542 and CcM 1506 differentiated 15 restorers and nine male sterile
lines as they produced amplicons sizes specific to rf locus and male
sterility locus.
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