BIODEGRADATION OF PINE NEEDLES (Pinus roxburghii) WITH MIXED CULTURE UNDER SOLID STATE FERMENTATION
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Date
2010
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ABSTRACT
The growing populations, rapid increase in industrialization, moderinization of
agricultural, house hold practices as well as transportation have tremendous impact on
our environment and this also increased the demand for energy especially for liquid fuel.
Various pretreatments of acid and alkali were given to pine needles in order to enhance
the rate of biodegradation, among all the pretreatments, NaOH+H2O2 were found to be
the best pretreatment. Bioconversion of pine needles into fermentable sugars by
employing potential cellulolytic and xylanolytic fungal cultures may be proven as a major
step in this direction.Three fungal culture were used for biodegradation and identified as
F. oxysporum, P. notatum and A. niger. Sets of three designs were used for
biodegradation i.e. single culture, culture consortium and sequential culture for
biodegradation of pine needles. Among the three sets, single culture (A.niger) showed
maximum biodegradation in terms of enzyme activity, biodegradation index and percent
hydrolysis. Biodegradation was apparently increased when tap water was replaced with
modified BSM. The enhanced biodegradation of pine needles can be utilized further for
bioconversion studies, which is prerequisite for any lignocellulosic waste utilizing biotech
industry.