DETECTION OF IL-4 AND IFN-γ γ γ γ γ mRNA CYTOKINE SECRETION FROM SENSITIZED CAPRINE ANTIGEN PRESENTING CELLS INDUCED CULTURE BY SEMI-QUANTITATIVE RT-PCR

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dc.contributor.authorAnanda Chitra, M
dc.contributor.authorRam, GC
dc.contributor.authorTANUVAS
dc.date.accessioned2020-06-15T07:43:52Z
dc.date.available2020-06-15T07:43:52Z
dc.date.issued2008
dc.descriptionTNV_IJCMIID_2008_29(1-2)59-62en_US
dc.description.abstractRT-PCR technique had been chosen to study the caprine antigen presenting cells induced Th cells cytokine secretion profile. Macrophages induced more IFN- γ γ γ γ γ than IL-4 cytokine mRNA and in contrast, B cells induced higher IL-4 production than IFN-γ γ γ γ γ. In general, IL-4 mRNAs were more in two antigen pulsed cultures as assessed by integrated densitometry analysis. M. paratuberculosis induced higher IFN-γ γ γ γ γ mRNAs than S. aureus. Dexamethasone (50μg/ml) increased the IL-4 production than indomethacin. Thus, RT-PCR based assay system for the detection of cytokine is a fast, economical method to assess simultaneously several cytokines during an immune response. But further studies are needed to develop a competitive RT-PCR assay system to quantify the cytokines secreted by caprine effector cells at various stages of infection.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810147619
dc.keywordsIL-4, mRNA cytokine secretion, caprine antigen, cultrue, semi-quantitative RT-PCR.en_US
dc.language.isoenen_US
dc.pages59-62en_US
dc.relation.ispartofseries;1
dc.subjectVeterinary Scienceen_US
dc.titleDETECTION OF IL-4 AND IFN-γ γ γ γ γ mRNA CYTOKINE SECRETION FROM SENSITIZED CAPRINE ANTIGEN PRESENTING CELLS INDUCED CULTURE BY SEMI-QUANTITATIVE RT-PCRen_US
dc.title.alternativeIndian J. Comp. Microbiol. Immunol. Infect. Dis.en_US
dc.typeArticleen_US
dc.volume29en_US
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