DETECTION OF IL-4 AND IFN-γ γ γ γ γ mRNA CYTOKINE SECRETION FROM SENSITIZED CAPRINE ANTIGEN PRESENTING CELLS INDUCED CULTURE BY SEMI-QUANTITATIVE RT-PCR
dc.contributor.author | Ananda Chitra, M | |
dc.contributor.author | Ram, GC | |
dc.contributor.author | TANUVAS | |
dc.date.accessioned | 2020-06-15T07:43:52Z | |
dc.date.available | 2020-06-15T07:43:52Z | |
dc.date.issued | 2008 | |
dc.description | TNV_IJCMIID_2008_29(1-2)59-62 | en_US |
dc.description.abstract | RT-PCR technique had been chosen to study the caprine antigen presenting cells induced Th cells cytokine secretion profile. Macrophages induced more IFN- γ γ γ γ γ than IL-4 cytokine mRNA and in contrast, B cells induced higher IL-4 production than IFN-γ γ γ γ γ. In general, IL-4 mRNAs were more in two antigen pulsed cultures as assessed by integrated densitometry analysis. M. paratuberculosis induced higher IFN-γ γ γ γ γ mRNAs than S. aureus. Dexamethasone (50μg/ml) increased the IL-4 production than indomethacin. Thus, RT-PCR based assay system for the detection of cytokine is a fast, economical method to assess simultaneously several cytokines during an immune response. But further studies are needed to develop a competitive RT-PCR assay system to quantify the cytokines secreted by caprine effector cells at various stages of infection. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810147619 | |
dc.keywords | IL-4, mRNA cytokine secretion, caprine antigen, cultrue, semi-quantitative RT-PCR. | en_US |
dc.language.iso | en | en_US |
dc.pages | 59-62 | en_US |
dc.relation.ispartofseries | ;1 | |
dc.subject | Veterinary Science | en_US |
dc.title | DETECTION OF IL-4 AND IFN-γ γ γ γ γ mRNA CYTOKINE SECRETION FROM SENSITIZED CAPRINE ANTIGEN PRESENTING CELLS INDUCED CULTURE BY SEMI-QUANTITATIVE RT-PCR | en_US |
dc.title.alternative | Indian J. Comp. Microbiol. Immunol. Infect. Dis. | en_US |
dc.type | Article | en_US |
dc.volume | 29 | en_US |
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