Crossability studies and genetic diversity in Blackgram using molecular markers
| dc.contributor.advisor | Khanna, V. K. | |
| dc.contributor.author | Singh, Puyam Tondonba | |
| dc.date.accessioned | 2023-10-13T11:45:58Z | |
| dc.date.available | 2023-10-13T11:45:58Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Blackgram productivity has remained low in subtropical and tropical Asia, it is important to estimate the genetic diversity in the existing cultivars in order to see if the lack of genetic variability might be the constraining factor. Crossability studies among cultivated blackgram (Vigna mungo) varieties and genetic diversity analysis using SSR markers. In the present study, a total of 35 blackgram accessions were used to evaluate the genetic diversity using 30 SSR markers. Out of these accessions, 5 random accessions were selected for the crossability studies. A total of 15 crosses were made and pollen fertility, pollen germinability and pollen tube growth rate was recorded after every 2 hours, 4 hours and 6 hours of pollination. These three parameters were used to generate a correlation matrix with pod set, to see if any abnormalities in these parameters is the cause of reduced pod set. The maximum pollen fertility between the five selected parents was observed in KU-16-33 (94.06 %) and minimum in GP-IPU-99-238 (90.35%). However, highest pod set was observed in PANT-U-6 (91.66 %). Upon selfing, maximum pollen germinability was found in PANT-U-31 (70.00%) and minimum was in PANT-U-6(62.66 %). Longest pollen tube growth was observed in PANT-U-6 (295.51 μm) and shortest in GP-IPU-99-238 (243.30μm). In case of crosses, maximum pollen germination was observed in GP-IPU-99-238 X PANT-U-6 (58.57%) and minimum in KU-16-33 X GPIPU-99-238 (51.33%). The highest pod set was observed in KU-16-33 X KU-16-26 (70.58%) and least was in KU-16-26 X GP-IPU-99-238 (54.55 %). Longest pollen tube was observed in crosses of KU-16-33 X KU-16-26 (244.11 μm) and minimum in GP-IPU-99-238 X PANT-U-31 (217.10 μm). A steady increase in pollen germination rate and pollen tube growth rate was recorded. The final reading in pollen tube growth revealed that pollen tube was almost 3-4 times than that of the initial stages. These data were subjected to Pearson’s correlation coefficient matrix which showed that there was non significant significant correlation between pollen fertility and pod set and high significant correlation was seen between pollen germinability, pollen tube growth rate with the podset. A total of 30 SSR primers were screened for genetic diversity analysis in 35 accesions of Blackgram out of which a total of 11 primers were found to be polymorphic. A total of 357 bands were generated and 119 of these bands were polymorphic with32.97% polymorphism and the rest 238 bands are monomorphic with 66.6% monomorphism. Based on the Polymorphism Information Content (PIC) value, two primers, CEDG 118 and CDEG 279 were found to be informative.The PIC value showed a range from 0.109 to 0.582. The observation recorded on all the molecular data were subjected to simple matching coefficient. The genetic similarity between the 35 accessions of blackgram was assessed by simple matching coefficient and it ranged from 0.14 to 0.78. The least genetic similarity was observed between the genotypes KU-16-30 and KU-16-33 with a coefficient of 0.82.These two accessions could be used for breeding purpose for crop improvement. The greatest similarity was observed between the accessions GP-PGRU-9514 and GP-134.16.50 748 having a coefficient of 0.14. These data were used to generate a Neighbor Joining tree. In the factorial analysis plot, the first axis in the plot explained 18.66 % variation, second, third, fourth and fifth components explained 12.36 %, 9.95 %, 7.66 % and 6.44 %, respectively among the 35 accessions of Blackgram. The five components explained a total of 55.17 % of total variation. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810198803 | |
| dc.language.iso | English | |
| dc.pages | 91p. | |
| dc.publisher | College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University , Imphal | |
| dc.sub | Genetics and Plant Breeding | |
| dc.theme | Academic Research | |
| dc.these.type | M.Sc | |
| dc.title | Crossability studies and genetic diversity in Blackgram using molecular markers | |
| dc.type | Thesis |