Verification of Soybean Varieties by SSR Markers
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Date
2013
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Publisher
JNKVV
Abstract
ABSTRACT
Soybean (Glycine max) is an important oilseed crop all over the world. The narrow genetic base of soybean makes variety characterization for registration and plant variety protection based on morphological descriptors difficult. Correct characterization of varieties could be achieved through distinguishable, uniform and stable expression of molecular markers.
Information on genetic diversity and relationship among breeding materials is essential to a plant breeder for an efficient crop improvement. Information on genetic marker for parental selection could have a favorable impact on breeding efficiency and it may contribute to achieve the goal genetic improvement in soybean in short period of time.
The accurate, fast, reliable, and cost-effective methods for identification of plant varieties and genetic purity of seed are essential in plant research, seed production, seed certification and in implementation of Protection of Plant Variety and Farmers Right Act. Every variety has a unique DNA fingerprint because of its genetic makeup and variations in DNA sequences. It has been extensively exploited as genetic marker for genome mapping in last four decades.
A total of 38 genotypes of soybean under active seed multiplication chain including all the varieties developed and released by JNKVV, Jabalpur were characterized with the help of 50 SSR markers.
The modified CTAB buffer method(without liquid nitrogen) of genom¬ic DNA extraction from soybean leaf tissues yielded quality DNA as yield by CTAB method involving liquid nitrogen. Out of 50 SSR primers 23 were found amplified and scorable. The SSR loci produced 51 alleles with an average of 2.2174 alleles per locus and a mean genetic diversity of 0.2339. The polymorphic information content (PIC) among genotypes varied from 0.5263 (Satt431) to 0.0499 (Satt243 and Satt337) with an average of 0.1998.
Pairwise coefficients of genetic similarity between all genotypes ranged from 0.56 (JS2 and NRC2, JS72-280 and PS1241) to 0.97 (Pratap Soya 2 and PS1042) with an average of 0.761. Out of all the 23 amplified markers, 16 markers were polymorphic while remaining 7 monomorphic (Satt183, Satt229, Satt269, Satt294, Satt326, Satt399 and Sct_187). Eight of the 23 SSR markers distributed on four linkage groups (I, J, K and L) were able to amplify ten unique alleles of 12 varieties viz., Bragg, Harasoya, JS20-34, JS72-280, JS75-46, JS76-205, JS90-41, JS93-05, JS97-52, MACS450, NRC2 and PS 1241.