MOLECULAR CHARACTERIZATION OF NL-1N STRAIN OF BCMV INFECTING COMMON BEAN IN HIMACHAL PRADESH AND DEVELOPMENT OF DIAGNOSTIC KITS
| dc.contributor.advisor | Sharma, P.N. | |
| dc.contributor.author | ANURADHA | |
| dc.date.accessioned | 2016-08-05T15:18:22Z | |
| dc.date.available | 2016-08-05T15:18:22Z | |
| dc.date.issued | 2014 | |
| dc.description | Doctoral Dissertation | en_US |
| dc.description.abstract | Common bean (Phaseolus vulgaris L.), locally known as “Rajmash” one of the most widely grown grain legume crop grown around the world is vulnerable to the attack of wide range of plant pathogens. Among these bean mosaic disease caused by Bean common mosaic virus (BCMV), a member of family Potyviridae is most important to low yield contributing factor because of its regular occurrence and ubiquitous seed borne nature. In India, five strains of BCMV viz., NL-1, NL-1n, NL-4, NL-7 and NL-7n have been reported from north-western Himalayas, of which NL-1n is widely prevalent in the entire region. The present investigations on BCMV- NL-1n strain were undertaken to obtain complete genome sequence, relationship with different strains and to elucidate the presence of R-genes to find out durable sources of resistance. Typical symptoms produced were mosaic, blistering, downward leaf rolling, green vein banding, thickening of leaves, reduction of leaf lamina and leaf deformation which were more severe on seed borne infected plants as compared to sap inoculated. DAS-ELISA test and coat protein gene amplification confirmed the identity of virus as BCMV and reaction pattern on International differential set of bean varieties established the strain identity. This strain was separated from other BCMV strains on the basis of temperature sensitivity assay as it induced temperature insensitive necrosis on cv. Jubila that contains resistance gene (I, bc-1), thus differs from all other strains of BCMV and BCMNV. The genome size was 10.081 kb and contains an open reading frame that encoded a single polyprotein of 3222 amino acids which cleaved into ten proteins typical of Potyvirus. Sequence homology, multiple alignment and phylogenetic analysis on nucleotide and amino acid sequences showed that BCMV-NL-1n is more closely related to BCMV-NL-1 though clustered along with other strains and isolates of BCMV. HC-Pro region sequences based comparison among BCMV strains showed its potential for the discrimination of different strains, however, it still needs further validation involving more number of isolates. Under artificial inoculation conditions, 130 common bean accessions were found resistant to strain BCMV-NL-1n in a panel of 304 accessions comprising of diverse germplasm. BCMV resistance gene prediction based on marker assisted selection (MAS) approach, using tightly linked SCAR markers showed the presence of three resistance genes viz., I, bc-12 and bc-3 genes in 105, 53 and 22 common bean accessions found resistant in the present study. Five genotypes viz., IC-43567, EC-109731, EC-116178, EC-120636 and Improved Tender Green possessed all the three genes. The resistant sources identified against the prevalent strains can be exploited in resistance breeding programme. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/71110 | |
| dc.language.iso | en | en_US |
| dc.publisher | CSK Himachal Pradesh Krishi Vishavavidyalaya, Palampur | en_US |
| dc.sub | Plant Pathology | |
| dc.subject | ##Unable to generate tags d8135.pdf | en_US |
| dc.subject | Bean | en_US |
| dc.subject | ##Unable to generate tags d8135.pdf | en_US |
| dc.subject | Plant Pathology | en_US |
| dc.subject | ##Unable to generate tags d8135.pdf | en_US |
| dc.subject | Rajmash | en_US |
| dc.these.type | Ph.D | |
| dc.title | MOLECULAR CHARACTERIZATION OF NL-1N STRAIN OF BCMV INFECTING COMMON BEAN IN HIMACHAL PRADESH AND DEVELOPMENT OF DIAGNOSTIC KITS | en_US |
| dc.type | Thesis | en_US |