ULTRASTRUCTURAL ANALYSIS OF RAT SPERMATOZOA FOLLOWING TREATMENT WITH CO-POLYMER STYRENE MALEIC ANHYDRIDE (SMA) IN VITRO

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dc.contributor.authorMonica, G
dc.contributor.authorUmamageswari, J
dc.contributor.authorSridevi, P; et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-03T12:01:12Z
dc.date.available2020-03-03T12:01:12Z
dc.date.issued2019-12
dc.descriptionTNV_35thVCRI_CA_Dec-2019_EPWO-27en_US
dc.description.abstractAdministration of co-polymer SMA dissolved in the vehicle Dimethyl Sulfoxide (DMSO), into the lumen of the vas deferens is a recent and very effective vas deferens-based fertility control procedure that blocks the lumen of the vas deferens and also impairs the fertilizing ability of the spermatozoa that pass in the vicinity of the lumen because of its electrical charge and pH-lowering action (Lohiya et al., 1998). In the present study, the micro structural alterations caused by treatment of rat spermatozoa with Styrene Maleic Anhydride (SMA) in vitro were analysed by Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). Spermatozoa recovered by mincing method from sacrificed adult male Wistar rats were utilized for the study. The recovered sperms were microscopically examined for their motility, diluted with TRIS buffer and incubated for 60 minutes with co-polymer SMA prepared by Reversible Addition-Fragmentation chain-Transfer (RAFT) polymerization technique@ 2 mg in 100 μl of DMSO. Washed epididymal sperms were fixed with 2.5% Glutaraldehyde in PBS (pH 7.4) for 30 min at 37°C and immobilized on 0.01% Poly- L-Lysine coated slides prior to analysis. Tapping mode AFM of untreated rat spermatozoa showed regularacrosome, mid-piece, post-acrosomal segment and flagellum that were clearly visible due to local height variations whereas SMA treated rat epididymal sperms revealed disintegrated surface topology with complete loss of smoothness. For SEM, a thin film of spermatozoa was smeared on a SEM stub with silver paint, airdried, sputter-coated with gold and observed under scanning electron microscope. Untreated spermatozoa had regular smooth sperm head with distinct acrosome, principle piece and flagellum whereas SMA treated spermatozoa revealed distorted acrosome. Hence, it is concluded that SMA can be a great breakthrough in canine contraception when injected through intravasal route which is already in phase III clinical trials in humans.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144154
dc.keywords35th Annual Convention of ISSAR, VCRI, Namakkalen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleULTRASTRUCTURAL ANALYSIS OF RAT SPERMATOZOA FOLLOWING TREATMENT WITH CO-POLYMER STYRENE MALEIC ANHYDRIDE (SMA) IN VITROen_US
dc.title.alternativeInternational Symposium on Global perspectives to enhance livestock fertility through modern reproductive techniques for doubling farmer's Incomeen_US
dc.typeOtheren_US
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