Detection of Yersinia spp. in ready-to-eat foods of animal origin.

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dc.contributor.advisorPanda, A.K.
dc.contributor.authorKoundal, Shubham
dc.date.accessioned2020-01-24T10:01:23Z
dc.date.available2020-01-24T10:01:23Z
dc.date.issued2019
dc.description.abstractThe present study was designed with the aim to determine the incidence of Yersinia spp. in ready-to-eat foods of animal origin. A total of 220 samples; RTE milk products (n=161), RTE meat products (n=21), beverages (n=18) and raw meat samples (n=20) were collected from 13 different places of Himachal Pradesh. In addition, 25 stool samples from hospitalized patients were also screened. Yersinia spp. was not detected in any of the samples tested by culture method using CIN agar. However, 48 other bacterial isolates i.e. Serratia spp. (6.5%, 16/48), Pseudomonas spp. (6.5%, 16/48), Enterobacter spp. (5.7%, 14/48) and Klebsiella spp. (0.8%, 2/48) were recovered from the tested samples. Highest number of isolates were detected in beverages (33%) followed by dahi based products (30%), milk based sweets (21.5%) and paneer based products (13.6%). None of the RTE meat products showed bacterial contamination. All isolates were susceptible to amikacin, tobramycin and doripenem. High level of susceptibility was observed for levofloxacin (95.8%), gentamicin (95.8%), imipenem (93.7%), ciprofloxacin (93.7%), cefepime (89.5%) and ampicillin/sulbactum (81.2%). 5 (10.4%) isolates were found to be multidrug resistant. Three isolates were resistant to imipenem while two isolates were in intermediate susceptibility range for doripenem. 47.9% isolates tested were positive for ESBL and 52.0% isolates as ESBL negative. Only one isolate from raw pork was positive for blaCTX-M-1 gene by molecular testing. In the present study no Yersinia spp. could be detected. However, other bacterial spp. belonging to Enterobacteriaceae were detected and some of them were multidrug resistant strains. Isolates resistant to carbapenem class of antibiotics were also detected which possess serious public health concern.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810142049
dc.keywordsYersinia sppen_US
dc.language.isoenen_US
dc.pages65en_US
dc.publisherCSK HPKV, Palampuren_US
dc.subVeterinary Public Healthen_US
dc.subjectnullen_US
dc.themeTo study the public health significance of this food borne pathogen.en_US
dc.these.typeM.V.Sc.en_US
dc.titleDetection of Yersinia spp. in ready-to-eat foods of animal origin.en_US
dc.typeThesisen_US
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