MOLECULAR SURVEY AND CHARACTERIZATION OF TOBACCO LEAF CURL VIRUS INFECTING TOBACCO
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Date
2016-07-22
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Tobacco leaf curl is one of the most common and serious viral disease in
Karnataka. Survey conducted during 2014-15 to assess the leaf curl disease in tobacco
growing areas viz., Mysuru, Shivamogga and Chamarajanagar revealed the occurrence of
disease in the range of 12.75 to 70.69 per cent. Among the districts surveyed, highest
average disease incidence was recorded in Mysuru (65.37%) and least incidence was
recorded in Chamarajanagar (42.95%). Tobacco leaf curl isolates collected from surveyed
areas were tested by PCR using Deng Primers. The virus was detected by coat protein
(CP) specific primers, which amplified ~780 bp. The sequence of CP of TbLCV-My-
[Akk] shared nucleotide identity of 88% with Jatropha Leaf curl virus and 87% with
Indian Cassava Mosaic virus isolate whereas TbLCV-Sh-[Hal] shared maximum identity
of 95% with Tomato leaf curl Karnataka virus isolate. Phylogenetic analysis of CP
sequences of both isolates revealed that TbLCV-My from Akkihebbal isolate from
Mysuru district(TbLCV-Sh-[Akk]) clustered with Indian cassava mosaic virus isolate
and TbLCV-Sh from Haladahalli isolate from Shivamogga district (TbLCV-Sh-[Hal])
clustered with Tomato leaf curl Karnataka virus isolate. TbLCV beta DNA satellite
molecule of ~1.3 kb was amplified only from TbLCV-Sh-[Hal] and shared 94%
nucleotide sequence identity with Papaya Leaf Curl beta satellite molecule. Thus
indicates that there is an involvement of two distinct begomoviruses in causing tobacco
leaf curl disease in southern Karnataka. Tobacco leaf curl virus isolate from Shivamogga
(TbLCV-Sh-[Hal] was successfully transmitted to tobacco, tomato, chilly, sunflower,
Euphorbia, Mirablis jalapa and Ageratum conizoides through whiteflies (Bemisia
tabaci).
July, 2016
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