Studies on variability in Rhizoctonia solani (Kuhn) causing root rot on cotton

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dc.contributor.advisorMan Mohan
dc.contributor.authorMehta, Akshit
dc.date.accessioned2022-01-19T05:11:13Z
dc.date.available2022-01-19T05:11:13Z
dc.date.issued2021-09
dc.description.abstractThe present investigations entitled “ Studies on variability in Rhizoctonia solani (Kuhn) causing root rot on Cotton.” was conducted for morphological, chemo-sensitivity and molecular variability was carried out with twelve isolates of R. solani collected from northern region of India. The isolates were identified on the basis of their morpho-cultural characteristics. All the isolates of R. solani exhibited variation in their colony colour, colony growth and pattern of sclerotia. In cultural and morphological variability maximum radial growth after 74 hours was observed in FTB Rs10 (84.3mm) and was fastest among all the isolates. Mycelial width varied significantly from 4.92 μm (BWN Rs5) to 7.33 μm (PWL Rs 2). Significant variation was observed in mean size of sclerotia, where the size varied between 1.16mm to 1.97mm and sclerotia colour varied significantly viz. olive brown, light and dark brown. A significant positive Karl Pearson‘s Correlation coefficient of 0.604 was found between sclerotia initiation and full growth of petri plate. No significant correlation was found between other cultural and morphological characters. In molecular variability, isolates FTB Rs10 and PWL Rs2 varied significantly. The Internal transcribed spacer ITS primers were used for the identification of R. solani and SSR primers exhibited the genetic variability among the isolates and generated a wide range of amplicons from 0.25 kb to 3 kb bp. Carbendazim 50% was found significantly effective even at lower concentrations (50 ppm), Thiophanate methyl 70% was not found much effective up to 100 ppm whereas, captan 50% was least effective at all the concentrations. The inhibition of mycelial growth noticed in T. harzianum (85.55%) that was significantly superior to T. viride (79.25%). In future, it is likely these two isolates of Rhizoctonia solani can be used in the breeding programme of cottonen_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810180978
dc.keywordsRhizoctonia solani, Tricoderma harzianum, T. virideen_US
dc.language.isoEnglishen_US
dc.pages45 + viiien_US
dc.publisherCCSHAU, Hisaren_US
dc.subPlant Pathologyen_US
dc.themeStudies on variability in Rhizoctonia solani (Kuhn) causing root rot on cotton.en_US
dc.these.typeM.Scen_US
dc.titleStudies on variability in Rhizoctonia solani (Kuhn) causing root rot on cottonen_US
dc.typeThesisen_US
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