TRANSFAUNATION USING LYOPHILISED RUMEN INOCULUM TO COUNTERACT RUMINAL FERMENTATION DISORDERS SEQUEL TO HIGH GRAIN RATION IN SMALL RUMINANTS
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Date
2021
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Abstract
A study with four experiments was carried out to design a protocol to lyophilise the rumen inoculums of goats and to study the effect of transfaunation of the lyophilised goat rumen inoculum into the rumen of goats having fermentation disorders sequel to high grain diet. Experiment 1 was executed using 5 × 3 × 3 factorial design with six replications in three runs to lyophilize the rumen fluid of goats. Four different cryoprotectants (10 % skim milk powder, 10 % skim milk powder + 5 % sodium glutamate solution, 5 % glycerol solution, 5 % DMSO solution and no cryoprotectant) at three pre freezing time intervals (2, 24 and 48 hours), at three freeze drying time intervals (8, 24 and 32 hours) were assessed. Pre freezing (-80℃ deep freezer) duration of 48 hours with 32 hours of time duration in lyophilizer (-45℃) was found to be ideal for lyophilizing goat rumen fluid with or without the addition of various cryoprotectants. The viability of rumen microbes in the “lyophilised goat rumen inoculum” produced with different cryoprotectents were assessed in experiment II through in vitro gas production studies and significantly (P < 0.05) highest gas production (11.00 ml / 0.2 g of substrate), in vitro apparent dry matter degradability (70.60 %), true dry matter degradability (76.81 %), microbial biomass (6.21 %) and partition factor