STUDIES ON POKKAH BOENG DISEASE OF SUGARCANE INCITED BY FUSARIUM SPP.

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dc.contributor.advisorANIL KUMAR, P.
dc.contributor.authorMANJULA
dc.date.accessioned2022-08-11T09:16:04Z
dc.date.available2022-08-11T09:16:04Z
dc.date.issued2022-08
dc.descriptionSTUDIES ON POKKAH BOENG DISEASE OF SUGARCANE INCITED BY FUSARIUM SPP.en_US
dc.description.abstractThe present investigation on “Studies on Pokkah boeng disease of sugarcane incited by Fusarium spp.” was conducted at Department of Plant Pathology, Bapatla and at RARS, Anakapalle during 2020-21, where 44 fields running across 15 villages in seven mandals of Visakhapatnam district, A.P were surveyed, Pokkah boeng incidence was recorded upto a maximum of 40% indicating impact of disease. All the twenty isolates obtained from the surveyed villages showed pathogenicity by producing Pokkah boeng symptoms such as malformed spindle leaves, red specks or stripes on chlorotic area and shot holes. Cultural characterization for these isolates showed variation in colony colour, reverse pigmentation, growth habit, nature and texture of mycelium. Variability in morphological characters were noticed such as macro and micro conidial length and width along with their shape, presence or absence of chlamydospore, mono or polyphialidec conidiophore. ITS marker with amplicon size ~500 bp generally used for fungi and TEF-α primer with amplicon size ~300 bp confirmed the isolates as Fusarium sacchari for 19 out of 20 isolates, where isolate F 14 which was found to be F. andiyazi through BLAST analysis. Phylogenetic tree using both ITS and TEF-α primer showed remarkable evolutionary relationship between these two species by forming two separate clades with 40% and 62 % similarity, respectively. F. andiyazi, a new species on sugarcane causing Pokkah boeng disease was first time noticed in India. Based on in-vitro poisoned food technique against F. sacchari, fungicides such as carbendazim, difenoconazole, tebuconazole, propiconazole, azoxystrobin + difenoconazole and trifloxystrobin + tebuconazole were found to be highly effective with 100% inhibition of radial growth of mycelium, both at 500 and 1000 ppm concentrations followed by azoxystrobin + tebuconazole. Azoxystrobin, copper oxychloride and mancozeb alone showed poor inhibition. xiv In vitro dual culture studies with bacterial bioagents (isolated from sugarcane phylloplane) against F. sacchari revealed significant efficacy of ScANKP-9 and ScANKP-13 isolates with 50.33 and 46.67 per cent inhibition of radial growth of pathogen respectively. These effective bacterial isolates were confirmed as Bacillus amyloliquefaciens (ScANKP-9) and Pseudomonas fluorescence (ScANKP-13) respectively, through molecular characterization. Studies on screening of sixty two sugarcane germplasm entries for resistance against Pokkah boeng disease with three highly susceptible check varieties revealed that six from IVT entries (CoV 18357, CoOr 18346, CoA 92081(c), CoC 01061 (c), CoOr 03151 (c) and CoA 11321 (c)), one from AVT І early entries (CoA 17321), one from AVT ІІ early entries (CoC 16337), three from AVT ІІ mid late entries (CoV 92102, Co 86249, Co 06030), five from PYT entries (2017A 268, 2017A 405, 2017A 408, 2017A 416, 2017A 269) and eleven from MYT entries (2016A 128, 2016A 275, 2016A 291, 2016A 332, 2016A 387, 2016A 737, 2016A 165, 2016A 254, 2016A 276, 2016A 286, 2016A 743) were found to be resistant with less than 5% disease incidence. Varieties such as CoV 18356 (IVT entries), CoC 17336 (AVT I Early entries), CoV 16356 (AVT II Early entries), CoC 16339 (AVT II Mid late entries) were highly susceptible with more than 20% disease incidence.en_US
dc.identifier.otherD6244
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810186228
dc.keywordsSTUDIES, POKKAH BOENG DISEASE, SUGARCANE, FUSARIUM SPP.en_US
dc.language.isoEnglishen_US
dc.pages160en_US
dc.publishergunturen_US
dc.subPlant Pathologyen_US
dc.themeSTUDIES ON POKKAH BOENG DISEASE OF SUGARCANE INCITED BY FUSARIUM SPP.en_US
dc.these.typeM.Scen_US
dc.titleSTUDIES ON POKKAH BOENG DISEASE OF SUGARCANE INCITED BY FUSARIUM SPP.en_US
dc.typeThesisen_US
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