Development of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogs

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dc.contributor.authorSubathra, M
dc.contributor.authorSenthilkumar, TMA
dc.contributor.authorRamadass, P
dc.contributor.authorDhinakar Raj, G
dc.contributor.authorTANUVAS
dc.date.accessioned2020-05-29T08:57:05Z
dc.date.available2020-05-29T08:57:05Z
dc.date.issued2011
dc.descriptionTNV_CIMID_2011_34(1)17en_US
dc.description.abstractAn IgG-ELISA used recombinant antigen and a rapid flow-through enzyme immunoassay were developed for rapid screening of leptospiral antibodies in dogs using recombinant LipL41, which is one of the conserved outer membrane proteins in pathogenic leptospires as the coating antigen. Results from this study were compared with the standard microscopic agglutination test and found that the sensitivity and specificity of the enzyme-linked immunosorbent assay were 75.46% and 93.29% and whereas that of flow- through-based dot-immunobinding assay were 87.73% and 89.63%, respectively. Relative merits of these tests were also assessed. The flow-through-based dot-immunobinding assay was thus proved to be a valid screening test for canine leptospirosis.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810146675
dc.keywordsCanine leptospirosis Enzyme-linked immunosorbent assay Flow-through-based dot-immunobinding assay Microscopic agglutination test Recombinant LipL41en_US
dc.language.isoenen_US
dc.pages17en_US
dc.relation.ispartofseries;1
dc.titleDevelopment of rapid flow-through-based dot-immunoassay for serodiagnosis of leptospirosis in dogsen_US
dc.title.alternativeComparative Immunology, Microbiology and Infectious Diseasesen_US
dc.typeArticleen_US
dc.volume34en_US
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