DIVERSITY, MULTIPLICATION AND SHELF LIFE ASSESSMENTOF TRICHODERMASPP. ISOLATED FROM RHIZOSPHERIC SOIL OF CARNATION
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Date
2014
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UHF,NAUNI
Abstract
ABSTRACT
Carnation is one of the most important cut flowers known in the world. But due to some biotic and
abiotic stresses its cultivation has declined gradually. Biotic factors which include some soil borne pathogens
such as Fusarium oxysporum Schl. f.sp. dianthi (Prill. and Del.) Snyd. and Hans. and Rhizoctonia solani Kuhn
are playing key role in decreasing the plant healthas well as the production of quality flowers. Keeping in mind
the ill effects by continuous use of chemicals, alternative disease management strategies have been evolved. The
biological management of soil borne diseases in recent years is increasingly gaining status as a possible practical
and safe approach. In order to use the biological agents in field effectively, their mass production on suitable
substrates is essential. Species of Trichoderma are most commonly exploited fungus as a biological control
agent and the present study involves the diversity study on morphologically and molecular basis. The
multiplication and shelf life assessment of procured and native Trichoderma species were studied on various
liquid PDB (Potato Dextrose Broth), MYE (Malt Yeast Extract) and solid substrates such as vermicompost,
FYM, cocopeat, wheat straw and rice straw. Three species from procured isolates namely Trichoderma viride
(85.9), Trichoderma harzianum (80.3%) and Trichodrema virens(80.04%) showed highest antagonistic activity
upto 85.9% against Fusarium and Rhizoctonia pathogens by giving maximum inhibition percentage in mycelia
growth under in vitroconditions. However, amongst native isolates T. viride(S1), T. harzianum(SR1) and T.
piluliferum (SR4) performed better over the rest of isolates. These potential Trichoderma spp. were further
grown on best solid medium (wheat straw alone and solid media supplemented with 3% jaggery and 10% wheat
flour at pH 6.5 and 25±1
o
C temperature). The highest population count of Trichoderma species was observed in
wheat straw with 10% wheat flour at pH 6.5 which was again applied with 5g/kg antioxidant crops after six
month of storage and was found that after addition of antioxidant crops shelf life of propagules of Trichoderma
species increased to greater extent in soybean, maize and brown rice. Of all the isolates tested, three species ( T.
harzianum, T. viride and T.hamatum) and one native isolate T. viride(S1) performed better and can be used in
the management of devastating diseases of carnation.
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