Variability studies in bipolaris sorokiniana (Sacc.) shoem causing leaf blight of wheat

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dc.contributor.advisorKarwasra, S.S.
dc.contributor.authorChauhan, Prashant Kumar
dc.date.accessioned2016-12-01T13:55:35Z
dc.date.available2016-12-01T13:55:35Z
dc.date.issued2005
dc.description.abstractBipolaris sorokiniana (Sacc.) Shoem (Teleomorph: Cochliobolus sativus (Ito and Kuribayashi, Drechs ex. Dastur) causes leaf blight of wheat. A total of 13 isolates of B. sorokiniana obtained from blighted leaf samples collected from different agroclimatic zones of India (NWPZ-Pant Nagar, Karnal, Hisar and Ludhiana; NEPZ-Faizabad, Dharwad, Coochbehar and Samastipur; CZ-Pune and Vijapur and NHZ-Almora). Morphological, pathological and genetic variability were studied. Nine characters were studied at morphological level of study of pathogen viz. spore and sporophore size, sporulation, per cent germination, colony growth, septation, color of cytoplasm, shape of spore, spore and sporophere color and nuclear staining. Two type of colors of spores viz. light brown and dark brown were observed whereas, sporophores showed different types of colour viz. light brown, dark brown, dark olivaceous, light to darkbrown and grayish brown. Maximum septa in spores were found in isolate BS-6, while the maximum sporophore septa were in BS-2 and BS-8. Similarly four types of cytoplasm colors i.e. light blue, blue, greenish blue and brown were observed whereas three types of shape of spores were observed namely elliptical, oblong and slightly curved. Fastest colony growth was observed in isolate BS-11 whereas, BS-5 was the slowest growing. Maximum sporulation was observed in BS-13 while minimum in BS-5. Among all spores and sporopores, the longest were in isoalate BS-8 whereas, shortest in BS-2. Isolate BS-2 showed maximum spore germination whereas, minimum in BS-1. All the spores represented high degree of variability when nuclear staining was done with ethidium bromide (2, 7-diamino-9 phenyl phenanthridium bromide). The pathological variability was studied at five characters viz. incubation period, infection response (IR), number of lesions on flag-2 leaf, necrotic area development and terminal disease severity. Differential infection response (susceptible, moderately susceptible, moderately resistant and resistant) were observed at seedling stage on differential set of five genotypes viz. Sonalika, GW322, HD2733, PBW-34 (d) and HPW184. Maximum virulence was observed in isolate BS-2 in respect to four pathological characters namely incubation period, number of lesions on flag-2 leaf, necrotic area development and terminal disease severity. Out of 20 primers, only two i.e. OPAA-15 and OPAA-16 produced the band of all 13 isolates of B. sorokiniana isolates and a high degree of variability was detected. The isolate BS-5 made a separate group when cluster analysis was done. Isolate BS-9 and BS-11 made another group and rest were in third group.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/88639
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subPlant Pathologyen_US
dc.these.typeM.Sc
dc.titleVariability studies in bipolaris sorokiniana (Sacc.) shoem causing leaf blight of wheaten_US
dc.typeThesisen_US
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