Studies on heterosis, combining ability and seed quality parameters in cucumber (Cucumis sativus L.)
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Date
2014-01
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present investigation was carried out at Vegetable Research Centre during 2011-12. Assessment of seed vigour parameters and biochemical characterization was performed in the NAIPLaboratory, Department of Vegetable Science, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India. The major objectives of this study were to know variability parameters, heritability, genetic advance; extent of heterosis, combining ability in crosses between genotypes of cucumber along with study of seed quality parameters and biochemical characterization. Analysis of variance revealed highly significant among the genotypes for fourteen characters, which clearly indicated that there was enough variability for these traits among the genotypes utilized for present investigation. The best parents identified as promisingly general combiner were PCUC-126, PCPGR-4273 and Pant Khira-1. The good specific combiners were PCUC-117 x PCUC-208, PCUC-117 x PCUC-133, PCPGR-4273 x PCUC-126 and PCUC-59 x Pant Khira-1 for different character. These
hybrids are also noted for having significantly higher SCA effects for yield contributing character which culminated in higher total yield. The cross PCUC-117 x Pant Khira-1 exhibits higher heterobeltiosis and standard heterosis for earliness. For yield contributing characters the crosses PCUC-117 x PCUC-133, PCUC-117 x PCUC-208, PCPGR-4273 x PCUC-104 recorded highest heterobeltiosis and standard heterosis. For seed yield the crosses PCUC-133 x PCUC-208, PCPGR-4273 x PCUC-133, PCUC-117 x Pant Khira-1 recorded highest heterobeltiosis and standard heterosis. Further study of seed quality parameters and establishment of its genetic purity is one of the pertinent issues resurgent in present times.
In addition to this a series of laboratory tests were also conducted involving eight pure line genotypes and twenty-eight hybrids to determine the effect of abiotic stresses (moisture and salt) on seed quality parameters. Under moisture stress and salt stress test, it was found that germination per cent, seedling length and vigour index decreased significantly as level of stress increased. The vigour parameters revealed PCPGR-4273, Pant Khira-1, PCUC-126 and PCUC-59 as superior pureline genotypes and PCUC-126 x PCUC-117, PCUC-126 x Pant Khira-1 PCUC-126 x PCUC-133 PCUC-117 x PCUC-59 as superior hybrids. The seed protein profile of parental genotypes revealed twenty-one protein bands which were divided into different zones and subzones according to their light and dark bands.
Description
Thesis-PhD
Keywords
heterosis, combining ability, seed quality, cucumbers, genetic parameters, crosses, biochemistry, characterization, genotypes